FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9907662
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5077465.124781204687847No Hit
CCCCCCACTGCTAAATTTGACTG4924004.96989097932489No Hit
AAGCTGCCAGTTGAAGAACTGT4598954.641811559578839No Hit
TTCAAGTAATCCAGGATAGGCT4158164.196913459502353No Hit
TAGCAGCACGTAAATATTGGCG4104304.14255149196652No Hit
TAGCTTATCAGACTGATGTTGAC3501453.53408301575084No Hit
AACATTCAACGCTGTCGGTGAGT3264833.2952577510213814No Hit
TAGCTTATCAGACTGATGTTGA3004083.032077598125572No Hit
TAGCTTATCAGACTGATGTTGACT2835522.8619466429113145No Hit
CATAAAGTAGAAAGCACTACT2304672.326149196450182No Hit
CCCCCACTGCTAAATTTGACTG2104882.1244971820798892No Hit
AACATTCAACGCTGTCGGTGAG1656041.6714740571489015No Hit
TGTAAACATCCTTGACTGGAAGCT1306371.318545182506226No Hit
TGAGGTAGTAGGTTGTATAGTT1190961.2020595777288323No Hit
TATTGCACTTGTCCCGGCCTGTAA840350.8481819424199171No Hit
TATTGCACTTGTCCCGGCCTGTAT829510.8372409151624268No Hit
TATTGCACTTGTCCCGGCCTGT768750.7759146406084504No Hit
TGAGGTAGTAGATTGTATAGTT752380.7593920745378677No Hit
TTCAAGTAATTCAGGATAGGTT738590.7454735537001566No Hit
AGCAGCATTGTACAGGGCTATGA713580.7202304640590282No Hit
CGCCTGTCTGAGCGTCGCT565280.5705483291618143No Hit
GCCTGTCTGAGCGTCGCT511300.5160652432430577No Hit
TGTAAACATCCTACACTCTCAGCT496470.5010970297533364No Hit
TGTAAACATCCCCGACTGGAAGCT492360.49694872513818095No Hit
CCCATAAAGTAGAAAGCACTAC491900.49648443800363806No Hit
ACTGGACTTGGAGTCAGAAGGC488820.4933757328419157No Hit
TATTGCACTTGTCCCGGCCTGTA460460.4647514216774856No Hit
CATTGCACTTGTCTCGGTCTGA414960.41882736815204236No Hit
TGTCAGTTTGTCAAATACCCCAA413120.4169702196138706No Hit
CCCATAAAGTAGAAAGCACT399960.4036875702865116No Hit
TAGCTTATCAGACTGATGTTGACA398060.40176986255687763No Hit
TGAGGTAGTAGTTTGTACAGTT387190.39079855570365646No Hit
CAACACCAGTCGATGGGCTGT378910.3824413872818835No Hit
TCGTACGACTCTTAGCGG355250.35856087944865295No Hit
CAACGGAATCCCAAAAGCAGCTGT347210.35044594779272853No Hit
TTATAAAGCAATGAGACTGATT331530.3346198124239604No Hit
TTCTCACTACTGCACTTGACTAG315190.31812752594910887No Hit
TGTAAACATCCTACACTCAGCT313300.316219911418052No Hit
TTCACAGTGGCTAAGTTCCG299680.3024729749561501No Hit
TTCAAGTAATCCAGGATAGGCTT265610.2680854474042413No Hit
AACATTCAACGCTGTCGGTGAGTT257080.25947594901804283No Hit
TGTCAGTTTGTCAAATACCCCA256830.2592236190536173No Hit
TGATGTAGTAGGTTGTATAGTT251160.2535007754604467No Hit
AACCGGGCGGAAACACCA234540.23672587942543863No Hit
TTCACAGTGGCTAAGTTCCGC233140.23531283162465574No Hit
CTTCTCACTACTGCACTTGACTAG231030.2331831667249044No Hit
TGAGGTAGTAGTTTGTGCTGTT216690.218709519965457No Hit
TCCTGTCTGAGCGTCGCT214710.21671106664720696No Hit
TAGCAGCACGTAAATATTGGC207700.20963573444471562No Hit
TTCAAGTAATCCAGGATAGGC207050.20897967653720925No Hit
GGAATACCGGGTGCTGTAGGCTTT198230.20007747539227722No Hit
TAGCAGCACGTAAATATTGGCGT189900.19166984097761913No Hit
CAAAGAATTCTCCTTTTGGGCT187870.18962092166648398No Hit
TAGCTTATCAGACTGATGTTG177460.17911390194780566No Hit
CAAAGAATTCTCCTTTTGGGCTT175750.17738796499113516No Hit
TGTAAACATCCCCGACTGGAAGC168940.1705144967601842No Hit
TACGCCTGTCTGAGCGTCGCT168800.17037319198010592No Hit
AGCAGCATTGTACAGGGCTATGT164130.16565966824463732No Hit
CTACGCCTGTCTGAGCGTCGCT163090.1646099755926272No Hit
TGATGTAGTAGATTGTATAGTT161620.1631262754018052No Hit
CAACGGAATCCCAAAAGCAGCT161230.1627326406573014No Hit
TGTCAGTTTGTCAAATACCCC160020.16151136362948193No Hit
CATAAAGTAGAAAGCACTAC158740.1602194342116233No Hit
TAGCAGCACATAATGGTTTGT156510.1579686509289477No Hit
TGCAAGTAATCCAGGATAGGCT142350.1436766817438867No Hit
TTCACAGTGGCTAAGTTCTGC142010.14333351299226801No Hit
ACGCCTGTCTGAGCGTCGCT140840.1421526087587566No Hit
CACCCGGCTGTGTGCACATGTGC140350.1416580420284826No Hit
CACTAGATTGTGAGCTCCTGGA138590.13988163907892698No Hit
GGAATACCGGGTGCTGTAGGCTT137400.13868054844826155No Hit
AACATTCAACGCTGTCGGTGA135020.13627836718693068No Hit
ACTGGACTTGGAGTCAGAAGG134630.13588473244242688No Hit
TATTGCACTTGTCCCGGCCTGTT134380.13563240247800137No Hit
ACCACAGGGTAGAACCACGGAC131910.1331393824294773No Hit
TTCACAGTGGCTAAGTTCTG131150.13237229933762373No Hit
CCCCCACTGCTAAATTTGACTGGCTT130490.1317061482315404No Hit
TGTAAACATCCTTGACTGGAAGC128260.12945536494886484No Hit
CAAAGTGCTGTTCGTGCAGGT125610.12678066732595442No Hit
AGCAGCATTGTACAGGGCTATG124960.12612460941844808No Hit
TGTCAGTTTGTCAAATACC122390.1235306573841538No Hit
ACTGGACTTGGAGTCAGAAGGCA122000.12313702263965No Hit
GCGTTCGCGCTTTCCCCTG121810.12294525186668662No Hit
CAGTGCAATAGTATTGTCAAAGC121660.1227938538880313No Hit
ATCACATTGCCAGGGATTTCC121020.12214788917910199No Hit
CCCATAAAGTAGAAAGCACTA119340.12045223181816256No Hit
CCCCCCACTGCTAAATTTGACTGG118570.11967505552773197No Hit
AACGGAATCCCAAAAGCAGCTG117590.11868592206718397No Hit
ACCGGGTGCTGTAGGCTT114810.11588001286277226No Hit
ACTTGACTTGGAGTCAGAAGGC111910.11295298527543632No Hit
TGTCAGTTTGTCAAATACCC110930.11196385181488833No Hit
CATAAAGTAGAAAGCACTACTA108990.11000577129094634No Hit
CCCCCCACTGCTAAATTTGACTGGCT108920.1099351189009072No Hit
TAGCTTATCAGACTGATGTTGACC108700.10971306853221274No Hit
ACTGGACTTGGAGTCAGAAGGCT107650.1086532826816256No Hit
CACTAGATTGTGAGCTCCTGGAA103510.10447469847073912No Hit
TGAGGTAGTAGGTTGTATAGT103390.10435358008781487No Hit
TTTAAACATCCTTGACTGGAAGCT103010.10397003854188808No Hit
AGCTCGGTCTGAGGCCCCTCAGT101820.10276894791122265No Hit
AACATTCAACGCTGTCGGTGAGA100160.10109347694743724No Hit
CAAAGTGCTGTTCGTGCAGGTAG100050.10098245176309002No Hit
CTCCCACTGCTTCACTTGACTAG99370.10029611425985262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)