FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7644180
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG118380015.486291531596589No Hit
CAACGGAATCCCAAAAGCAGCTG7382929.658223642038779No Hit
CCCCCACTGCTAAATTTGACTG3966045.188313200369431No Hit
AACATTCAACGCTGTCGGTGAGT2284922.989097587968886No Hit
AACATTCAACGCTGTCGGTGAG1917822.50886295194514No Hit
TAGCTTATCAGACTGATGTTGACT1711642.239141412159316No Hit
TAGCTTATCAGACTGATGTTGAC1666212.1797105771972927No Hit
TAGCAGCACGTAAATATTGGCG1616332.1144583199244393No Hit
TTCAAGTAATCCAGGATAGGCT1531152.0030271396016315No Hit
TATTGCACTTGTCCCGGCCTGTAA1484981.9426282478957848No Hit
AAGCTGCCAGTTGAAGAACTGT1426131.8656415730660452No Hit
TATTGCACTTGTCCCGGCCTGTAT1343321.75731079069305No Hit
TAGCTTATCAGACTGATGTTGA1088331.42373675135855No Hit
GGAATACCGGGTGCTGTAGGCTTT780341.0208289182096706No Hit
TGAGGTAGTAGGTTGTATAGTT759680.9938018204699522No Hit
AGCAGCATTGTACAGGGCTATGA562450.7357885345452357No Hit
TATTGCACTTGTCCCGGCCTGTA558400.730490386149986No Hit
TGTAAACATCCTTGACTGGAAGCT516920.6762268811043173No Hit
TATTGCACTTGTCCCGGCCTGT513880.6722499993459076No Hit
TGAGGTAGTAGATTGTATAGTT506750.6629226418006902No Hit
ACTGGACTTGGAGTCAGAAGGC486930.6369944192836904No Hit
GGAATACCGGGTGCTGTAGGCTT465540.6090123466480382No Hit
TGTAAACATCCCCGACTGGAAGCT425290.5563579088927785No Hit
TAGCTTATCAGACTGATGTTGACA411590.5384357772841561No Hit
TTAATGCTAATCGTGATAGGGGTT390490.5108330782372995No Hit
CAACGGAATCCCAAAAGCAGCT356800.4667603326975555No Hit
CAACGGAATCCCAAAAGCAGCTGT349740.4575245480875647No Hit
GCCTGTCTGAGCGTCGCT303120.3965369732266901No Hit
CACCCGGCTGTGTGCACATGTGC299370.39163128026812555No Hit
TTCAAGTAATTCAGGATAGGTT290400.3798968627112391No Hit
CCCCCACTGCTAAATTTGACTGGCTT287610.37624702715006714No Hit
CATTGCACTTGTCTCGGTCTGA260900.341305411437198No Hit
CATAAAGTAGAAAGCACTACT260500.3407821375216178No Hit
TGAGAACTGAATTCCATAGGCTGT254880.3334301390077157No Hit
ACCCCCCACTGCTAAATTTGACTG223790.29275867391924315No Hit
CACTAGATTGTGAGCTCCTGGAA218000.2851842839912195No Hit
CACTAGATTGTGAGCTCCTGGA216010.2825809962612079No Hit
CCCATAAAGTAGAAAGCACTAC210860.27584384459811256No Hit
CCCATAAAGTAGAAAGCACT186750.24430350933651485No Hit
CAAAGAATTCTCCTTTTGGGCT184370.24119002953881252No Hit
TATTGCACTTGTCCCGGCCTGTAG175090.22905007469735142No Hit
ACTGGACTTGGAGTCAGAAGGCA174360.22809509980141757No Hit
CTTCTCACTACTGCACTTGACTAG171190.2239481540204443No Hit
TGAGGTAGTAGTTTGTACAGTT161590.21138958004651903No Hit
AACGGAATCCCAAAAGCAGCTG161060.21069624210837526No Hit
TGTAAACATCCTACACTCTCAGCT155370.20325267065924665No Hit
TAGCAGCACGTAAATATTGGC154950.2027032330478874No Hit
TATTGCACTTGTCCCGGCCTGTAAT153810.20121190238848377No Hit
CCCCCCACTGCTAAATTTGACTGG150210.19650243714826182No Hit
ACTGGACTTGGAGTCAGAAGGCT146220.19128277984034914No Hit
TTCTCACTACTGCACTTGACTAG146000.19099497918678002No Hit
TGAATACCGGGTGCTGTAGGCTTT145020.18971295809360847No Hit
CAACACCAGTCGATGGGCTGT144160.188587919175111No Hit
AACATTCAACGCTGTCGGTGAGTT139780.1828580697995076No Hit
CCCCCCACTGCTAAATTTGACTGGCT132900.17385775845152782No Hit
TGAGAACTGAATTCCATGGGTT132410.17321674790494204No Hit
TGAGGTAGTAGTTTGTGCTGTT131930.1725888192062458No Hit
TATTGCACTTGTCCCGGCCTGTAAA128920.16865118299150467No Hit
CACCCGTAGAACCGACCTTGCG127860.16726450711521706No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC120990.1582772776151268No Hit
CCCCCCACTGCTAAATTTGACTGT119000.15567398988511522No Hit
CAAAGAATTCTCCTTTTGGGCTT117340.15350240313545732No Hit
GGAATACCGGGTGCTGTAGGCTTA116250.1520764817155012No Hit
TATTGCACTTGTCCCGGCCTGTT115910.15163169888725803No Hit
AGCAGCATTGTACAGGGCTATGT115470.15105609758011979No Hit
CTCCCACTGCTTCACTTGACTAG111950.14645128712301383No Hit
TTCAAGTAATCCAGGATAGGCTT109480.14322007069430598No Hit
CCCCCCACTGCTAAATTTGACTGTA104690.13695386555523287No Hit
TGTAAACATCCCCGACTGGAAGC103590.13551486228738727No Hit
TATTGCACTTGTCCCGGCCTGTTT96680.12647530539573898No Hit
ATGCACCTGGGCAAGGATTCTGA96470.12620058659005937No Hit
CTCCCACTGCTTCACTTGACTAGCCT95580.12503630212789338No Hit
TCGTTTCCCGGCCAATGCACCA90100.11786744948444439No Hit
TAGCTTATCAGACTGATGTTGACC90080.11784128578866536No Hit
CAACGGAATCCCAAAAGCAGCTGA87650.11466239675151553No Hit
TGAATACCGGGTGCTGTAGGCTT86190.11275244695964773No Hit
AATGCACCCGGGCAAGGATTCT86110.11264779217653169No Hit
TTATAAAGCAATGAGACTGATT81490.10660397845158015No Hit
ACTGGACTTGGAGTCAGAAGG80980.10593680420921538No Hit
TCGTACGACTCTTAGCGG80180.10489025637805494No Hit
AGCTCGGTCTGAGGCCCCTCAGT79510.10401377256945805No Hit
CCCATAAAGTAGAAAGCACTA79470.10396144517790004No Hit
GCCCCCACTGCTAAATTTGACTG76660.10028544592094901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)