FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10409975
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6990676.715357145430224No Hit
CAACGGAATCCCAAAAGCAGCTG4832724.642393473567419No Hit
TTCAAGTAATCCAGGATAGGCT3709853.563745350012848No Hit
TAGCTTATCAGACTGATGTTGA3281583.152341864413699No Hit
TAGCAGCACGTAAATATTGGCG3251153.123110286047757No Hit
AAGCTGCCAGTTGAAGAACTGT3009152.8906409477448314No Hit
CCCCCACTGCTAAATTTGACTG2961412.844781087370527No Hit
TAGCTTATCAGACTGATGTTGACT2940492.8246849776296292No Hit
TAGCTTATCAGACTGATGTTGAC2776242.6669036188847715No Hit
AACATTCAACGCTGTCGGTGAGT2432112.3363264561154087No Hit
AACATTCAACGCTGTCGGTGAG2178442.0926467162505196No Hit
TGAGGTAGTAGATTGTATAGTT1752281.6832701327332678No Hit
TATTGCACTTGTCCCGGCCTGTAT1715731.6481595777127225No Hit
CATAAAGTAGAAAGCACTACT1682251.6159981171904831No Hit
TGAGGTAGTAGGTTGTATAGTT1575081.5130487825379024No Hit
TTCAAGTAATTCAGGATAGGTT1553391.4922129976296774No Hit
GCCTGTCTGAGCGTCGCT1471041.4131061794096529No Hit
TATTGCACTTGTCCCGGCCTGTAA1349571.2964200202209901No Hit
TGTAAACATCCTTGACTGGAAGCT1110961.0672071738885058No Hit
TATTGCACTTGTCCCGGCCTGT978250.9397236784910625No Hit
TATTGCACTTGTCCCGGCCTGTA727290.698647210968326No Hit
TCCTGTCTGAGCGTCGCT659760.6337767381766046No Hit
TGAGAACTGAATTCCATAGGCTGT637990.6128641039003455No Hit
TTAATGCTAATCGTGATAGGGGTT601800.5780993710359535No Hit
TGATGTAGTAGATTGTATAGTT584900.5618649420387657No Hit
TGAGGTAGTAGTTTGTACAGTT580510.5576478329678985No Hit
AGCAGCATTGTACAGGGCTATGA554850.532998398170985No Hit
GGAATACCGGGTGCTGTAGGCTTT532880.5118936404746409No Hit
TGATGTAGTAGGTTGTATAGTT522940.5023451064964133No Hit
TGTAAACATCCCCGACTGGAAGCT472560.45394921697698604No Hit
TAGCTTATCAGACTGATGTTGACA452340.43452553920638615No Hit
TGAGAACTGAATTCCATGGGTT410980.3947944159327952No Hit
TTCTCACTACTGCACTTGACTAG410800.39462150485471864No Hit
CATTGCACTTGTCTCGGTCTGA389840.3744869704298041No Hit
CAACACCAGTCGATGGGCTGT355630.3416242594242541No Hit
TTATAAAGCAATGAGACTGATT351500.3376569107994976No Hit
GGAATACCGGGTGCTGTAGGCTT339080.32572604641221525No Hit
CCCATAAAGTAGAAAGCACTAC335150.32195082120754376No Hit
TGAATACCGGGTGCTGTAGGCTTT296470.2847941517630926No Hit
ACTGGACTTGGAGTCAGAAGGC290920.27946272685573215No Hit
CAAAGAATTCTCCTTTTGGGCT284990.27376626745021No Hit
TGAGGTAGTAGTTTGTGCTGTT279030.26804098953167516No Hit
CAACGGAATCCCAAAAGCAGCTGT274880.2640544285649101No Hit
TGTAAACATCCTACACTCTCAGCT261630.2513262519842747No Hit
AACCGGGCGGAAACACCA245610.2359371660354612No Hit
TTCAAGTAATCCAGGATAGGCTT239330.2299044906447902No Hit
CACCCGGCTGTGTGCACATGTGC230250.22118208737292835No Hit
TTCACAGTGGCTAAGTTCCG227030.21808890030955885No Hit
CTTCTCACTACTGCACTTGACTAG226840.21790638306047805No Hit
TAGCAGCACGTAAATATTGGC219780.21112442633147532No Hit
TTCACAGTGGCTAAGTTCCGC205790.19768539309652522No Hit
TATTGCACTTGTCCCGGCCTGTT200860.19294955079142842No Hit
TGATAACTGAATTCCATAGGCTGT197500.18972187733399937No Hit
TGTCAGTTTGTCAAATACCCCA196960.18920314409976968No Hit
TAGCTTATCAGACTGATGTTG196900.18914550707374417No Hit
TTCAAGTAATCCAGGATAGGC192820.1852261893040089No Hit
TGATGTAGTAGTTTGTACAGTT190810.18329534893215402No Hit
CACTAGATTGTGAGCTCCTGGA190320.1828246465529456No Hit
TATTGCACTTGTCCCGGCCTGTAG189140.18169111837444374No Hit
CACTAGATTGTGAGCTCCTGGAA186480.17913587688731242No Hit
TGAATACCGGGTGCTGTAGGCTT186030.17870359919212103No Hit
TGAGGTAGTAAGTTGTATTGTT179930.1728438348795266No Hit
CAAAGAATTCTCCTTTTGGGCTT177760.17075929577160368No Hit
CCCATAAAGTAGAAAGCACT175810.16888609242577432No Hit
CTCCCACTGCTTCACTTGACTAG171440.1646881956969157No Hit
TATTGCACTTGTCCCGGCCTGTTT169970.1632760885592905No Hit
TGAGGTAGTAGATTGTATAGT168670.16202728632873759No Hit
TTCACAGTGGCTAAGTTCTG165930.15939519547357223No Hit
TCGTACGACTCTTAGCGG161140.15479383956253498No Hit
AGCTCGGTCTGAGGCCCCTCAGT158940.15268048194159928No Hit
CAACGGAATCCCAAAAGCAGCT155730.14959690104923404No Hit
TGTAAACATCCCCGACTGGAAGC154690.14859785926479172No Hit
TGTCAGTTTGTCAAATACCCCAA153720.14766606067737914No Hit
TGTCAGTTTGTCAAATACC142790.13716651576973046No Hit
ATCCCGGACGAGCCCCCA141950.13635959740537323No Hit
TGAGGTAGTAGGTTGTATAGT140980.13542779881796066No Hit
CCCCCACTGCTAAATTTGACTGGCTT138940.13346813993309303No Hit
AACATTCAACGCTGTCGGTGAGTT137550.13213288216350183No Hit
AGCAGCATTGTACAGGGCTATGT135900.13054786394780005No Hit
TTCACAGTGGCTAAGTTCTGC135200.12987543197750237No Hit
ACCGGGTGCTGTAGGCTTA133600.12833844461682184No Hit
TTCAAGTAATTCAGGATAGGTTA132600.12737782751639654No Hit
TGATAACTGAATTCCATGGGTT129530.12442873301809082No Hit
TGTCAGTTTGTCAAATACCCC127920.12288213948640607No Hit
ACCCCCCACTGCTAAATTTGACTG125530.12058626461638956No Hit
TTCAAGTAATTCAGGATAGGT121550.11676300855669683No Hit
TGAGAACTGAATTCCATGGGTTG120220.11548538781313115No Hit
TGTCAGTTTGTCAAATACCC118610.1139387942814464No Hit
ACCGGGTGCTGTAGGCTT115700.11114339851920874No Hit
TTCAAGTAATTCAGGATAGGTTT113980.1094911371064772No Hit
TATTGCACTTGTCCCGGCCTGTAAT112320.10789651271977119No Hit
TGTAAACATCCTACACTCAGCT110750.10638834387210344No Hit
CCCCCCACTGCTAAATTTGACTGG109680.10536048357464836No Hit
AGCTACATCTGGCTACTGGGTCTC107690.10344885554480199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)