FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8260374
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7321938.86392068930535No Hit
GCCTGTCTGAGCGTCGCT5980997.240580148065935No Hit
CAACGGAATCCCAAAAGCAGCTG5112296.188932849771693No Hit
CCCCCACTGCTAAATTTGACTG2664433.225556130993584No Hit
AACATTCAACGCTGTCGGTGAGT2217042.68394627168213No Hit
TCCTGTCTGAGCGTCGCT2216542.6833409722126382No Hit
AACATTCAACGCTGTCGGTGAG2014352.438569972739733No Hit
TTCAAGTAATCCAGGATAGGCT1903362.304205596502047No Hit
AAGCTGCCAGTTGAAGAACTGT1733092.0980769151614687No Hit
TAGCAGCACGTAAATATTGGCG1438501.7414465737265648No Hit
TATTGCACTTGTCCCGGCCTGTAT1329201.6091281096957593No Hit
TATTGCACTTGTCCCGGCCTGTAA1105221.337978159342422No Hit
TAGCTTATCAGACTGATGTTGACT1027291.2436361840275028No Hit
TAGCTTATCAGACTGATGTTGAC978701.184813181582335No Hit
TATTGCACTTGTCCCGGCCTGT958411.1602501291103768No Hit
TAGCTTATCAGACTGATGTTGA937361.1347670214447916No Hit
TATTGCACTTGTCCCGGCCTGTA771210.9336260077328218No Hit
TGAGGTAGTAGGTTGTATAGTT762050.9225369214517406No Hit
TGAGGTAGTAGATTGTATAGTT560180.6781533136393098No Hit
TTAATGCTAATCGTGATAGGGGTT545200.6600185415333495No Hit
GGAATACCGGGTGCTGTAGGCTTT520530.630153065708647No Hit
CATAAAGTAGAAAGCACTACT448710.543207849910912No Hit
TCGTACGACTCTTAGCGG443920.5374090809931851No Hit
TGTAAACATCCTTGACTGGAAGCT420450.508996323895262No Hit
TTCAAGTAATTCAGGATAGGTT407140.49288325201740263No Hit
AGCAGCATTGTACAGGGCTATGA401710.4863096997787268No Hit
GGAATACCGGGTGCTGTAGGCTT400170.4844453774126934No Hit
ATCCCGGACGAGCCCCCA339250.4106956900498694No Hit
ACTGGACTTGGAGTCAGAAGGC319350.38660477116411435No Hit
TGTAAACATCCCCGACTGGAAGCT311800.3774647491747952No Hit
CCCATAAAGTAGAAAGCACT279050.3378176339231129No Hit
TGAGAACTGAATTCCATAGGCTGT274740.33259995249609764No Hit
ACCGGGTGCTGTAGGCTTA272440.3298155749364375No Hit
ACCGGGTGCTGTAGGCTT269190.32588112838474387No Hit
CCCATAAAGTAGAAAGCACTAC269060.3257237505226761No Hit
CATTGCACTTGTCTCGGTCTGA266470.322588299270711No Hit
CAACGGAATCCCAAAAGCAGCTGT249690.30227444907458184No Hit
CAACACCAGTCGATGGGCTGT243010.29418764816217763No Hit
CACCCGGCTGTGTGCACATGTGC236540.28635507302695984No Hit
TGAGGTAGTAGTTTGTACAGTT209510.2536325837062583No Hit
TGATGTAGTAGGTTGTATAGTT207030.2506302983375813No Hit
CGCCTGTCTGAGCGTCGCT199980.24209557581775354No Hit
TGAATACCGGGTGCTGTAGGCTTT197150.23866958082043258No Hit
TATTGCACTTGTCCCGGCCTGTT196590.2379916454146023No Hit
ACCGGGTGCTGTAGGCTTT191960.23238657232711254No Hit
AACCGGGCGGAAACACCA183990.22273809878342068No Hit
TGAGAACTGAATTCCATGGGTT180000.21790780901687987No Hit
TTCTCACTACTGCACTTGACTAG177790.21523238536172817No Hit
CACTAGATTGTGAGCTCCTGGA165980.20093521189234292No Hit
TAGCTTATCAGACTGATGTTGACA164350.19896193562180114No Hit
TATTGCACTTGTCCCGGCCTGTAG159780.19342949847065036No Hit
CTCCCACTGCTTCACTTGACTAG153970.18639591863516106No Hit
TGAATACCGGGTGCTGTAGGCTT153300.18558481734604268No Hit
TGATGTAGTAGATTGTATAGTT148750.18007659217367156No Hit
CACTAGATTGTGAGCTCCTGGAA147530.17859966146811268No Hit
TCCGGTCTGAGCGTCGCT146080.17684429300658783No Hit
CAACGGAATCCCAAAAGCAGCT144550.17499207662994437No Hit
AGCTCGGTCTGAGGCCCCTCAGT144370.1747741688209275No Hit
AACATTCAACGCTGTCGGTGAGTT142520.17253456078380955No Hit
ACCCCCCACTGCTAAATTTGACTG136200.16488357548943908No Hit
TGAGGTAGTAGTTTGTGCTGTT135560.1641087921684902No Hit
TTCACAGTGGCTAAGTTCCG128380.15541669178659465No Hit
CAAAGAATTCTCCTTTTGGGCT120800.14624035182910605No Hit
TGTAAACATCCTACACTCTCAGCT119070.14414601566466603No Hit
TATTGCACTTGTCCCGGCCTGTTT118980.1440370617601576No Hit
CTTCTCACTACTGCACTTGACTAG116690.14126479018988727No Hit
AACGGAATCCCAAAAGCAGCTG113690.1376329933729393No Hit
GCCGGTCTGAGCGTCGCT112310.13596236683714322No Hit
ACTGGACTTGGAGTCAGAAGGCA111910.13547812726155015No Hit
CCCCCACTGCTAAATTTGACTGGCTT110860.13420699837561834No Hit
AGCTACATCTGGCTACTGGGTCTC110380.13362591088490666No Hit
TTCAAGTAATCCAGGATAGGC108000.1307446854101279No Hit
TAGCAGCACGTAAATATTGGC106420.1288319390865353No Hit
CCCCCCACTGCTAAATTTGACTGT104460.1264591651661293No Hit
CCCCCCACTGCTAAATTTGACTGG104000.12590228965419725No Hit
CCCTGTCTGAGCGTCGCT101780.12321476000965574No Hit
ACTGGACTTGGAGTCAGAAGGCT99670.12066039624840232No Hit
TTCAAGTAATCCAGGATAGGCTT98730.11952243324575862No Hit
ACTTGACTTGGAGTCAGAAGGC96670.11702859943145431No Hit
GGAATACCGGGTGCTGTAGGCTTA92350.11179881201504918No Hit
TTCACAGTGGCTAAGTTCTG91660.11096349874715117No Hit
CCCGCCACTGCTAAATTTGACTG91390.11063663703362583No Hit
AGCAGCATTGTACAGGGCTATGT91320.11055189510789706No Hit
TTATAAAGCAATGAGACTGATT90730.10983764173389728No Hit
TATTGCACTTGTCCCGGCCTGA87870.10637532876840686No Hit
TGAGGTAGTAGGTTGTATAGT87120.10546737956416985No Hit
AGCTACATTGTCTGCTGGGTTT84460.10224718638647598No Hit
TGTAAACATCCCCGACTGGAAGC83760.1013997671291881No Hit
ACTGGACTTGGAGTCAGAAGG82810.10024969813715458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)