FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872130
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8546129.632545961341865No Hit
CCCCCCACTGCTAAATTTGACTG6934797.816375549050791No Hit
CCCCCACTGCTAAATTTGACTG2906443.2759213401967733No Hit
AACATTCAACGCTGTCGGTGAGT2818913.1772640842728856No Hit
TTCAAGTAATCCAGGATAGGCT2667673.0067976911970407No Hit
TAGCAGCACGTAAATATTGGCG2411892.7185016450390154No Hit
AACATTCAACGCTGTCGGTGAG2349432.6481014142038046No Hit
AAGCTGCCAGTTGAAGAACTGT2029352.287331227112317No Hit
GCCTGTCTGAGCGTCGCT1826392.0585699262747506No Hit
TAGCTTATCAGACTGATGTTGA1791282.0189965656499624No Hit
TAGCTTATCAGACTGATGTTGAC1670411.8827609604458007No Hit
TATTGCACTTGTCCCGGCCTGTAT1623271.8296282854286399No Hit
TAGCTTATCAGACTGATGTTGACT1564181.7630264660233788No Hit
TATTGCACTTGTCCCGGCCTGTAA1378601.5538545986138617No Hit
TATTGCACTTGTCCCGGCCTGT1118811.2610387809917123No Hit
TGAGGTAGTAGGTTGTATAGTT959561.0815441162381525No Hit
TATTGCACTTGTCCCGGCCTGTA887501.000323484890325No Hit
CATAAAGTAGAAAGCACTACT847550.9552948390070931No Hit
TCCTGTCTGAGCGTCGCT723360.8153171786256513No Hit
TGAGGTAGTAGATTGTATAGTT632480.7128840537728821No Hit
TTCAAGTAATTCAGGATAGGTT605470.6824404060806142No Hit
AGCAGCATTGTACAGGGCTATGA526120.5930030330935186No Hit
TCGTACGACTCTTAGCGG523300.5898245404429376No Hit
TGTAAACATCCTTGACTGGAAGCT488500.550600588584703No Hit
CATTGCACTTGTCTCGGTCTGA479230.5401521393397075No Hit
CCCATAAAGTAGAAAGCACT441360.4974679135675424No Hit
ACTGGACTTGGAGTCAGAAGGC422670.4764019463195422No Hit
CAACACCAGTCGATGGGCTGT375380.4231002025443721No Hit
TGTAAACATCCCCGACTGGAAGCT353630.3985852326329754No Hit
CCCATAAAGTAGAAAGCACTAC339820.38301963564555525No Hit
CAACGGAATCCCAAAAGCAGCTGT335040.37763197789031494No Hit
CACCCGGCTGTGTGCACATGTGC330080.3720414376254631No Hit
TAGCTTATCAGACTGATGTTGACA275310.31030879845087933No Hit
ATCCCGGACGAGCCCCCA272110.30670199828000716No Hit
CACTAGATTGTGAGCTCCTGGA245020.2761681805834676No Hit
CAACGGAATCCCAAAAGCAGCT242050.2728206191748768No Hit
TATTGCACTTGTCCCGGCCTGTT235710.26567464633633636No Hit
TGAGGTAGTAGTTTGTACAGTT215880.24332375652746296No Hit
CACTAGATTGTGAGCTCCTGGAA205850.23201869224188554No Hit
AACGGAATCCCAAAAGCAGCTG193030.21756894905732896No Hit
TTCTCACTACTGCACTTGACTAG190340.21453698266368953No Hit
ACCGGGTGCTGTAGGCTT188770.2127673963298554No Hit
CCCCCACTGCTAAATTTGACTGGCTT187440.21126832000883666No Hit
TGAGGTAGTAGTTTGTGCTGTT184040.20743609482728498No Hit
AACATTCAACGCTGTCGGTGAGTT182420.20561015224078097No Hit
TTCACAGTGGCTAAGTTCCG180010.20289378086209287No Hit
CAAAGAATTCTCCTTTTGGGCT179100.2018680970635011No Hit
TATTGCACTTGTCCCGGCCTGTAG178890.2016314008022876No Hit
TGTAAACATCCTACACTCTCAGCT177830.20043664824568622No Hit
ACCGGGTGCTGTAGGCTTA177180.19970401696097778No Hit
TAGCAGCACGTAAATATTGGC176460.1988924869225316No Hit
TTATAAAGCAATGAGACTGATT165800.18687733385331368No Hit
TTCACAGTGGCTAAGTTCTG165560.1866068238404983No Hit
CTCCCACTGCTTCACTTGACTAG165500.18653919633729443No Hit
CTTCTCACTACTGCACTTGACTAG164480.18538952878282894No Hit
TTCAAGTAATCCAGGATAGGCTT159170.17940449474928793No Hit
TGATGTAGTAGGTTGTATAGTT158660.1788296609720552No Hit
TTCAAGTAATCCAGGATAGGC148000.1668145079028373No Hit
CCCATAAAGTAGAAAGCACTA147170.16587899410851734No Hit
TATTGCACTTGTCCCGGCCTGTTT146450.1650674640700711No Hit
GGAATACCGGGTGCTGTAGGCTTT144920.16334296273837284No Hit
TTCACAGTGGCTAAGTTCCGC143080.16126905264012137No Hit
AACCGGGCGGAAACACCA132180.14898338955808807No Hit
ACTGGACTTGGAGTCAGAAGGCT131350.14804787576376813No Hit
AGCAGCATTGTACAGGGCTATGT129610.14608667817085638No Hit
ACCCCCCACTGCTAAATTTGACTG129510.14597396566551663No Hit
ACTGGACTTGGAGTCAGAAGGCA129150.1455682006462935No Hit
TGTCAGTTTGTCAAATACCCCAA128000.14427200683488633No Hit
ACCGGGTGCTGTAGGCTTT124750.14060885041134427No Hit
TTAATGCTAATCGTGATAGGGGTT118010.1330120275514448No Hit
GGAATACCGGGTGCTGTAGGCTT117010.13188490249804724No Hit
ACTGGACTTGGAGTCAGAAGG115240.12988989115353358No Hit
TATTGCACTTGTCCCGGCCTGA112240.12650851599334095No Hit
AACATTCAACGCTGTCGGTGA112050.1262943622331954No Hit
ACTTGACTTGGAGTCAGAAGGC109260.12314968333421625No Hit
CCCCCCACTGCTAAATTTGACTGGCT105760.11920474564732482No Hit
TTCACAGTGGCTAAGTTCTGC104870.11820160434980101No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC104690.11799872184018945No Hit
CAAAGAATTCTCCTTTTGGGCTT104190.11743515931349068No Hit
ATCCCACCGCTGCCACCA102920.11600371049567577No Hit
TGATGTAGTAGATTGTATAGTT102670.11572192923232641No Hit
TGTCAGTTTGTCAAATACCCCA101460.11435810791771536No Hit
AGCAGCATTGTACAGGGCTATG100570.11335496662019154No Hit
TGTAAACATCCCCGACTGGAAGC98430.11094291900592078No Hit
TATTGCACTTGTCCCGGCCTGTAAA97940.110390627729756No Hit
TAGCTTATCAGACTGATGTTG97490.10988342145572709No Hit
ACCACAGGGTAGAACCACGGAC96930.10925223142582446No Hit
TATTGCACTTGTCCCGGCCTGTAAT93670.10557780375174847No Hit
TGAGGTAGTAGGTTGTATAGT92530.10429288119087525No Hit
CGCCCCACTGCTAAATTTGACTG92310.10404491367912778No Hit
CGCCTGTCTGAGCGTCGCT91610.1032559261417495No Hit
CCCCCCACTGCTAAATTTGACTGG90730.10226405609475965No Hit
AGCTCGGTCTGAGGCCCCTCAGT90180.101644137315391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)