FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9432252
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG94712610.04135597734242No Hit
CCCCCCACTGCTAAATTTGACTG8480158.990588885877942No Hit
GCCTGTCTGAGCGTCGCT4001414.242263671496478No Hit
TTCAAGTAATCCAGGATAGGCT3063603.248004824298587No Hit
AAGCTGCCAGTTGAAGAACTGT3019093.200815669471087No Hit
TAGCAGCACGTAAATATTGGCG2810472.9796383726813067No Hit
CCCCCACTGCTAAATTTGACTG2739642.9045449591465538No Hit
AACATTCAACGCTGTCGGTGAGT2389642.533477689103302No Hit
AACATTCAACGCTGTCGGTGAG2243252.378276152927212No Hit
TCCTGTCTGAGCGTCGCT1301491.3798295465388328No Hit
TATTGCACTTGTCCCGGCCTGTAA1137571.2060428410945763No Hit
TATTGCACTTGTCCCGGCCTGTAT1031581.0936730698034784No Hit
TAGCTTATCAGACTGATGTTGAC1002461.06280027293588No Hit
TAGCTTATCAGACTGATGTTGACT943901.0007154176966433No Hit
TGAGGTAGTAGGTTGTATAGTT896610.9505789285527995No Hit
TAGCTTATCAGACTGATGTTGA881020.9340505321528729No Hit
CATAAAGTAGAAAGCACTACT841460.8921093287159843No Hit
TATTGCACTTGTCCCGGCCTGT809390.8581089648580212No Hit
TGTAAACATCCTTGACTGGAAGCT687490.7288715356629573No Hit
CAACGGAATCCCAAAAGCAGCTGT617240.654393033604276No Hit
TTAATGCTAATCGTGATAGGGGTT580690.6156430086897593No Hit
TCGTACGACTCTTAGCGG549170.5822257505418643No Hit
TATTGCACTTGTCCCGGCCTGTA548810.5818440813498198No Hit
CCCATAAAGTAGAAAGCACTAC536930.5692489980123516No Hit
TGAGGTAGTAGATTGTATAGTT492420.5220598431848512No Hit
TGAGAACTGAATTCCATGGGTT474860.5034428681506813No Hit
CCCCCACTGCTAAATTTGACTGGCTT446880.47377869039122367No Hit
CTCCCACTGCTTCACTTGACTAG417280.44239700126756576No Hit
AGCAGCATTGTACAGGGCTATGA414200.4391316092911852No Hit
TTCAAGTAATTCAGGATAGGTT411910.4367037691529022No Hit
TGTAAACATCCCCGACTGGAAGCT399460.4235043762613636No Hit
TTCTCACTACTGCACTTGACTAG396140.41998453815695336No Hit
CCCATAAAGTAGAAAGCACT368530.3907126315115415No Hit
AACCGGGCGGAAACACCA357330.3788384788701574No Hit
ACTGGACTTGGAGTCAGAAGGC336450.35670166573157713No Hit
ACCGGGTGCTGTAGGCTT281890.29885757929283485No Hit
CATTGCACTTGTCTCGGTCTGA278200.2949454700743788No Hit
TGTAAACATCCTACACTCTCAGCT252760.26797418050323507No Hit
ACCGGGTGCTGTAGGCTTA247710.26262020989261103No Hit
CAACGGAATCCCAAAAGCAGCT240100.2545521472496706No Hit
ACCGGGTGCTGTAGGCTTT234570.24868928438298724No Hit
AACGGAATCCCAAAAGCAGCTG232440.246431074996724No Hit
ACCCCCCACTGCTAAATTTGACTG227560.24125733706012098No Hit
CCCCCCACTGCTAAATTTGACTGG221740.23508701845540175No Hit
TGAGGTAGTAGTTTGTACAGTT207560.22005349305764943No Hit
CTTCTCACTACTGCACTTGACTAG204060.21634282035721694No Hit
CACCCGGCTGTGTGCACATGTGC202190.21436026094298583No Hit
CAAAGAATTCTCCTTTTGGGCT192740.20434144465181803No Hit
CCCTGTCTGAGCGTCGCT192700.2042990369638131No Hit
CCCCCCACTGCTAAATTTGACTGGCT192260.2038325523957587No Hit
AGCTCGGTCTGAGGCCCCTCAGT192160.20372653317574638No Hit
TAGCAGCACGTAAATATTGGC185970.19716394345698143No Hit
TTCACAGTGGCTAAGTTCCG171430.18174874886718462No Hit
CCCATAAAGTAGAAAGCACTA168350.17848335689080402No Hit
TTCAAGTAATCCAGGATAGGC168170.1782925222947818No Hit
CTCCCACTGCTTCACTTGACTAGCCT159930.16955653856576353No Hit
AACATTCAACGCTGTCGGTGAGTT155670.16504011979323707No Hit
CAACACCAGTCGATGGGCTGT153300.1625274642789442No Hit
TATTGCACTTGTCCCGGCCTGTT149900.15892281079852405No Hit
GGAATACCGGGTGCTGTAGGCTTT148560.15750215325035843No Hit
TAGCTTATCAGACTGATGTTGACA142560.15114100004961697No Hit
TCGTTTCCCGGCCAATGCACCA138370.1466987947310992No Hit
CGCCTGTCTGAGCGTCGCT137580.1458612428930016No Hit
TATTGCACTTGTCCCGGCCTGTAG137100.14535235063694227No Hit
TGAGGTAGTAGTTTGTGCTGTT133270.141291814510469No Hit
TGTCAGTTTGTCAAATACCCCA130170.1380052186900859No Hit
TGATGTAGTAGGTTGTATAGTT121970.12931164264907258No Hit
TCCCACTGCTTCACTTGACTAG121770.12909960420904784No Hit
GGAATACCGGGTGCTGTAGGCTT121330.12863311964099347No Hit
ATCCCGGACGAGCCCCCA120390.1276365389728773No Hit
CACTAGATTGTGAGCTCCTGGA118230.1253465238206104No Hit
TGTAAACATCCCCGACTGGAAGC116220.123215537498362No Hit
TTCACAGTGGCTAAGTTCTG116010.12299289713633606No Hit
TCGTTTCCCGGGCGGCGCACCA115380.12232497605025819No Hit
TGAGGTAGTAGGTTGTGTGGTT113630.12046963970004194No Hit
CACTAGATTGTGAGCTCCTGGAA112980.11978051476996161No Hit
TTCAAGTAATCCAGGATAGGCTT110710.1173738784756811No Hit
ATCACATTGCCAGGGATTTCC106380.11278324624914601No Hit
CGCGACCTCAGATCAGACGTGG105950.11232736360309288No Hit
TGTAAACATCCTACACTCAGCT105290.11162763675101131No Hit
TTCACAGTGGCTAAGTTCTGC104710.11101272527493965No Hit
TTCACAGTGGCTAAGTTCCGC97050.10289165302199305No Hit
ACTGGACTTGGAGTCAGAAGGCT94560.10025177444368534No Hit
CTACGCCTGTCTGAGCGTCGCT94360.10003973600366063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)