FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8908868
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6428777.216146877470853No Hit
CCCCCCACTGCTAAATTTGACTG4333834.864624776121949No Hit
TTCAAGTAATCCAGGATAGGCT3365233.777393491518788No Hit
TAGCAGCACGTAAATATTGGCG3304053.7087203447171966No Hit
AAGCTGCCAGTTGAAGAACTGT3162573.5499122896421853No Hit
GCCTGTCTGAGCGTCGCT3159243.5461744410176466No Hit
AACATTCAACGCTGTCGGTGAGT2261682.538683927071318No Hit
CCCCCACTGCTAAATTTGACTG1989472.233134445363878No Hit
AACATTCAACGCTGTCGGTGAG1954782.194195715998935No Hit
TAGCTTATCAGACTGATGTTGA1515631.7012599131561945No Hit
CATAAAGTAGAAAGCACTACT1507191.6917862067324378No Hit
TAGCTTATCAGACTGATGTTGAC1460601.639490000300824No Hit
TGAGGTAGTAGGTTGTATAGTT1156181.2977855323482175No Hit
TAGCTTATCAGACTGATGTTGACT1140531.2802187662899482No Hit
TTCAAGTAATTCAGGATAGGTT910871.0224306836738406No Hit
TGAGGTAGTAGATTGTATAGTT814680.9144596148466898No Hit
TATTGCACTTGTCCCGGCCTGT803710.902146041449935No Hit
TATTGCACTTGTCCCGGCCTGTAA797820.8955346515404651No Hit
TGAGAACTGAATTCCATGGGTT792460.889518174475141No Hit
TGTAAACATCCTTGACTGGAAGCT751410.8434404909804478No Hit
TATTGCACTTGTCCCGGCCTGTAT710880.7979464955592562No Hit
TTAATGCTAATCGTGATAGGGGTT589780.6620145230572504No Hit
TATTGCACTTGTCCCGGCCTGTA458870.515071050553224No Hit
CAACGGAATCCCAAAAGCAGCT455670.5114791239470604No Hit
CCCATAAAGTAGAAAGCACT447890.502746252385825No Hit
ACCGGGTGCTGTAGGCTT438650.4923745643105274No Hit
CCCATAAAGTAGAAAGCACTAC421790.47344960100430267No Hit
AGCAGCATTGTACAGGGCTATGA402080.4513255780644634No Hit
CAACGGAATCCCAAAAGCAGCTGT400210.4492265459539865No Hit
TGAGGTAGTAGTTTGTACAGTT369360.41459812851644No Hit
CATTGCACTTGTCTCGGTCTGA357190.40093758264237384No Hit
ACCGGGTGCTGTAGGCTTT348240.3908914129157599No Hit
TTCTCACTACTGCACTTGACTAG337180.37847681658320675No Hit
TGTAAACATCCCCGACTGGAAGCT332700.37344811933457767No Hit
ACCGGGTGCTGTAGGCTTA307760.34545354134778966No Hit
TCCTGTCTGAGCGTCGCT286820.3219488716187062No Hit
ACTGGACTTGGAGTCAGAAGGC274390.30799648170788924No Hit
TTCACAGTGGCTAAGTTCCG269300.3022830734499602No Hit
CAACACCAGTCGATGGGCTGT258840.29054196335606275No Hit
TGTAAACATCCTACACTCTCAGCT256720.28816231197947934No Hit
AACCGGGCGGAAACACCA255900.28724188078664986No Hit
TCGTACGACTCTTAGCGG245760.2758599633533688No Hit
TAGCAGCACGTAAATATTGGC229730.25786665601061776No Hit
CTCCCACTGCTTCACTTGACTAG220100.24705720188019398No Hit
TCGTTTCCCGGCCAATGCACCA203970.22895164683100028No Hit
TGTCAGTTTGTCAAATACCCCA199550.22399029820623673No Hit
CCCCCACTGCTAAATTTGACTGGCTT196490.22055551838909276No Hit
AACGGAATCCCAAAAGCAGCTG195870.21985958260914854No Hit
CGCCTGTCTGAGCGTCGCT190890.21426964682830638No Hit
TTCAAGTAATCCAGGATAGGC187730.21072261930471975No Hit
TGAGGTAGTAGTTTGTGCTGTT182970.2053796284780513No Hit
TAGCTTATCAGACTGATGTTGACA181620.20386428444107602No Hit
CACCCGGCTGTGTGCACATGTGC174910.1963324633387766No Hit
TGTCAGTTTGTCAAATACCCCAA167240.1877230642546281No Hit
CCCATAAAGTAGAAAGCACTA164730.1849056468229185No Hit
TTCACAGTGGCTAAGTTCTG164440.18458012847423488No Hit
CAAAGAATTCTCCTTTTGGGCT155740.17481457801372743No Hit
TGTCAGTTTGTCAAATACC154390.17329923397675215No Hit
TTATAAAGCAATGAGACTGATT146310.16422961929618893No Hit
TGAGAACTGAATTCCATAGGCTGT145830.16369083030526438No Hit
TTCACAGTGGCTAAGTTCCGC143450.16101933489193015No Hit
AACATTCAACGCTGTCGGTGAGTT142700.16017747709361055No Hit
CTTCTCACTACTGCACTTGACTAG142310.15973971103848436No Hit
TTCACAGTGGCTAAGTTCTGC139070.15610288534974365No Hit
TTCAAGTAATCCAGGATAGGCTT136010.15266810553259966No Hit
TATTGCACTTGTCCCGGCCTGTT132260.14845881654100163No Hit
TGTAAACATCCTACACTCAGCT131910.14806594956845248No Hit
ATCCCGGACGAGCCCCCA131320.14740368810044105No Hit
AGCTCGGTCTGAGGCCCCTCAGT126190.14164538075993494No Hit
TGAGGTAGTAGGTTGTATAGT118210.13268801378581432No Hit
TGAGGTAGTAGGTTGTGTGGTT117820.1322502477306881No Hit
CCCCCCACTGCTAAATTTGACTGG117630.13203697708844717No Hit
TGAGAACTGAATTCCATGGGTTG116600.13088082571208823No Hit
AATGCACCCGGGCAAGGATTCT115570.1297246743357293No Hit
CGCGACCTCAGATCAGACGTGG114130.12810830736295567No Hit
AATGACACGATCACTCCCGTTG113170.12703072938110654No Hit
CAACGGAATCCCAAAAGCAGCTGA112970.12680623396822133No Hit
GGAATACCGGGTGCTGTAGGCTTT112850.1266715367204902No Hit
CTACGCCTGTCTGAGCGTCGCT112450.12622254589471973No Hit
TGTAAACATCCCCGACTGGAAGC101460.11388652295667642No Hit
GGAATACCGGGTGCTGTAGGCTT100990.11335895873639615No Hit
ATCACATTGCCAGGGATTTCC99590.11178749084619953No Hit
TGATGTAGTAGGTTGTATAGTT98560.11063133946984062No Hit
ACCCCCCACTGCTAAATTTGACTG98450.11050786699275372No Hit
CACTAGATTGTGAGCTCCTGGA97320.1092394679099522No Hit
AGAGGTAGTAGGTTGCATAGTT96740.10858843121258505No Hit
AGCAGCATTGTACAGGGCTATGT96680.10852108258871947No Hit
CAAAGTGCTGTTCGTGCAGGT95190.10684859176272452No Hit
CACCCGTAGAACCGACCTTGCG91890.10314441745011825No Hit
AACATTCAACGCTGTCGGTGA91640.1028637981840117No Hit
TATTGCACTTGTCCCGGCCTGTAG90050.10107905965157414No Hit
TGAGGTAGTAGATTGTATAGT89280.10021475231196601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)