FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7958264
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7877749.898817128961793No Hit
GCCTGTCTGAGCGTCGCT4982236.260448258564934No Hit
CCCCCCACTGCTAAATTTGACTG4006765.034716113966564No Hit
TCGTACGACTCTTAGCGG3038223.8176918986351795No Hit
TTCAAGTAATCCAGGATAGGCT2425653.04796372676252No Hit
TAGCAGCACGTAAATATTGGCG2351452.954727312388732No Hit
AAGCTGCCAGTTGAAGAACTGT2047262.572495710119694No Hit
AACATTCAACGCTGTCGGTGAGT2042152.5660747117713107No Hit
AACATTCAACGCTGTCGGTGAG1968532.473567099558396No Hit
CCCCCACTGCTAAATTTGACTG1713432.153019804319133No Hit
TAGCTTATCAGACTGATGTTGA1026541.2899044314187114No Hit
TAGCTTATCAGACTGATGTTGAC1012101.2717597707238664No Hit
CATAAAGTAGAAAGCACTACT885781.113031686307466No Hit
TGAGGTAGTAGGTTGTATAGTT873931.098141504227555No Hit
TAGCTTATCAGACTGATGTTGACT778810.9786179498443379No Hit
TTAATGCTAATCGTGATAGGGGTT662920.8329957387691588No Hit
TATTGCACTTGTCCCGGCCTGT615530.7734475760040129No Hit
TATTGCACTTGTCCCGGCCTGTAA613550.7709595962134455No Hit
TGTAAACATCCTTGACTGGAAGCT514180.6460956811686569No Hit
TATTGCACTTGTCCCGGCCTGTAT508520.6389835773228936No Hit
TATTGCACTTGTCCCGGCCTGTA441440.5546938377515498No Hit
CAACGGAATCCCAAAAGCAGCTGT427150.536737660374172No Hit
TTCAAGTAATTCAGGATAGGTT419680.5273511911643042No Hit
CCCATAAAGTAGAAAGCACTAC402080.5052358152481496No Hit
CCCATAAAGTAGAAAGCACT395480.49694254927959164No Hit
TGAGGTAGTAGATTGTATAGTT386600.48578433688553185No Hit
AGCAGCATTGTACAGGGCTATGA379010.47624708102169017No Hit
TGAGAACTGAATTCCATGGGTT356530.4479997145106018No Hit
ACCGGGTGCTGTAGGCTT342180.42996814380623716No Hit
CAACGGAATCCCAAAAGCAGCT331450.41648530383008153No Hit
TGTAAACATCCCCGACTGGAAGCT305050.3833122399558497No Hit
ACTGGACTTGGAGTCAGAAGGC278040.3493726772572511No Hit
CCCCCACTGCTAAATTTGACTGGCTT275760.34650773083174924No Hit
CATTGCACTTGTCTCGGTCTGA267010.33551287064616103No Hit
ACCGGGTGCTGTAGGCTTT257500.32356302831873884No Hit
TCCTGTCTGAGCGTCGCT245490.3084717973668629No Hit
CAACACCAGTCGATGGGCTGT241460.3034078789042434No Hit
AACGGAATCCCAAAAGCAGCTG212000.26638975535368015No Hit
TAGCAGCACGTAAATATTGGC211720.26603791982774133No Hit
CCCATAAAGTAGAAAGCACTA206480.25945356927088625No Hit
ACCGGGTGCTGTAGGCTTA189550.23818008550608524No Hit
CAAAGAATTCTCCTTTTGGGCT185890.2335810925598849No Hit
TTCACAGTGGCTAAGTTCCG185340.2328899870625051No Hit
TGTAAACATCCTACACTCTCAGCT176910.22229722462084694No Hit
TCGTTTCCCGGCCAATGCACCA174750.2195830648493189No Hit
TAGCTTATCAGACTGATGTTGACA172130.21629088957089135No Hit
AACCGGGCGGAAACACCA167950.21103848779080464No Hit
TTCTCACTACTGCACTTGACTAG165060.20740704254093603No Hit
AACATTCAACGCTGTCGGTGAGTT158990.1997797509607623No Hit
CTCCCACTGCTTCACTTGACTAG153020.19227811492556668No Hit
TGAGGTAGTAGTTTGTACAGTT147710.18560580548722685No Hit
CACCCGGCTGTGTGCACATGTGC134280.1687302657966612No Hit
TTCAAGTAATCCAGGATAGGC132810.16688312928548238No Hit
TGAGGTAGTAGTTTGTGCTGTT130620.16413127285046084No Hit
TTCACAGTGGCTAAGTTCTG128170.16105271199849613No Hit
TATTGCACTTGTCCCGGCCTGTT123100.1546819758681039No Hit
ACCCCCCACTGCTAAATTTGACTG111070.1395656138072323No Hit
TGAGGTAGTAGGTTGTGTGGTT107630.13524306305998393No Hit
CACTAGATTGTGAGCTCCTGGA107330.1348660964250495No Hit
CGCCTGTCTGAGCGTCGCT105260.13226502664400175No Hit
TGTCAGTTTGTCAAATACCCCA101370.12737702594435169No Hit
TTAATGCTAATCGTGATAGGGGT99820.12542936499719035No Hit
CCCCCCACTGCTAAATTTGACTGG97580.12261468078967977No Hit
GGAATACCGGGTGCTGTAGGCTTT96620.1214083875578895No Hit
CCCCCCACTGCTAAATTTGACTGGCT96110.12076754427850095No Hit
CTTCTCACTACTGCACTTGACTAG95780.12035288098007303No Hit
CGCGACCTCAGATCAGACGTGG95780.12035288098007303No Hit
ATCCCGGACGAGCCCCCA94590.11885757999483305No Hit
AGCAGCATTGTACAGGGCTATG93800.11786490118950566No Hit
CTCCCACTGCTTCACTTGACTAGCCT90470.11368057154173322No Hit
AGCTCGGTCTGAGGCCCCTCAGT89260.1121601394474976No Hit
CACTAGATTGTGAGCTCCTGGAA88690.11144390284112213No Hit
ATCACATTGCCAGGGATTTCC88640.11138107506863307No Hit
GGAATACCGGGTGCTGTAGGCTT88180.11080305956173357No Hit
TGTAAACATCCTACACTCAGCT84030.10558835444514028No Hit
TGAGGTAGTAGGTTGTATAGT81420.10230874472121054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)