FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6623922
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3308764.995167515559513No Hit
CCCCCCACTGCTAAATTTGACTG2799734.226695302269562No Hit
GCCTGTCTGAGCGTCGCT2781294.198856810210024No Hit
AAGCTGCCAGTTGAAGAACTGT2383863.5988648417055638No Hit
AACATTCAACGCTGTCGGTGAGT2121483.2027551049061262No Hit
TTCAAGTAATCCAGGATAGGCT1788822.700545084920988No Hit
TAGCAGCACGTAAATATTGGCG1642492.479633667183883No Hit
AACATTCAACGCTGTCGGTGAG1255101.8947988819916661No Hit
CCCCCACTGCTAAATTTGACTG1118051.6878972910610963No Hit
CGCCTGTCTGAGCGTCGCT986571.4894046155736738No Hit
TAGCTTATCAGACTGATGTTGAC840431.2687800369629956No Hit
TATTGCACTTGTCCCGGCCTGT778741.1756479016510157No Hit
TAGCTTATCAGACTGATGTTGA750001.1322597095799134No Hit
TCGTACGACTCTTAGCGG688921.0400484788317252No Hit
TGAGGTAGTAGGTTGTATAGTT645440.9744076092683459No Hit
ACCGGGTGCTGTAGGCTT608420.9185192700034813No Hit
TAGCTTATCAGACTGATGTTGACT603370.9108953879589765No Hit
AACCGGGCGGAAACACCA579870.875417917058806No Hit
TATTGCACTTGTCCCGGCCTGTAA568810.8587208605415342No Hit
CATAAAGTAGAAAGCACTACT545040.8228357761459147No Hit
TATTGCACTTGTCCCGGCCTGTAT525180.7928535390362387No Hit
TATTGCACTTGTCCCGGCCTGTA429600.6485583616473745No Hit
AGCAGCATTGTACAGGGCTATGA420670.6350769227053096No Hit
TGTAAACATCCTTGACTGGAAGCT419880.6338842758112188No Hit
ACCGGGTGCTGTAGGCTTT405130.6116165015228138No Hit
ACCGGGTGCTGTAGGCTTA392710.5928662807321705No Hit
TGAGGTAGTAGATTGTATAGTT371290.5605289434265681No Hit
TTCAAGTAATTCAGGATAGGTT321190.48489399482662987No Hit
ACTGGACTTGGAGTCAGAAGGC311240.4698726826795364No Hit
CCCATAAAGTAGAAAGCACT292450.4415058027555276No Hit
GCGTTCGCGCTTTCCCCTG285640.43122488459254205No Hit
ATCCCGGACGAGCCCCCA261520.394811412332452No Hit
TTCACAGTGGCTAAGTTCCG257190.38827449960914395No Hit
TGTAAACATCCCCGACTGGAAGCT243400.3674560177490013No Hit
TACGCCTGTCTGAGCGTCGCT240880.36365162512481275No Hit
CAACACCAGTCGATGGGCTGT228790.3453995986063846No Hit
CCCATAAAGTAGAAAGCACTAC223000.3366585536484276No Hit
ACGCCTGTCTGAGCGTCGCT217890.3289440908271565No Hit
CATTGCACTTGTCTCGGTCTGA216870.3274042176221278No Hit
CAACGGAATCCCAAAAGCAGCTGT203280.3068876716845398No Hit
AACATTCAACGCTGTCGGTGAGTT201340.3039588932357597No Hit
CTACGCCTGTCTGAGCGTCGCT196300.2963501079873827No Hit
CTCCCACTGCTTCACTTGACTAG183760.2774187256432065No Hit
TGTAAACATCCTACACTCTCAGCT179610.2711535552501977No Hit
TTCTCACTACTGCACTTGACTAG173800.2623823166999853No Hit
GGAATACCGGGTGCTGTAGGCTTT164640.2485536514469826No Hit
TGAGGTAGTAGTTTGTACAGTT163500.24683261668842116No Hit
GGAATACCGGGTGCTGTAGGCTT152290.22990910822923336No Hit
TATTGCACTTGTCCCGGCCTGTT123300.18614349625493778No Hit
ATCCCACCGCTGCCACCA123140.18590194751689407No Hit
TTCACAGTGGCTAAGTTCTG123080.1858113667401277No Hit
TGAGGTAGTAGTTTGTGCTGTT122290.18461871984603684No Hit
AGCTCGGTCTGAGGCCCCTCAGT121700.18372800887450064No Hit
CAACGGAATCCCAAAAGCAGCT119530.18045200411478277No Hit
TGTAAACATCCTACACTCAGCT114780.17328102595410996No Hit
CGCGACCTCAGATCAGACGTGG113820.17183173352584769No Hit
AACGGAATCCCAAAAGCAGCTG110090.16620062857020357No Hit
AGCAGCATTGTACAGGGCTATG108190.16333223730593446No Hit
TGAGGTAGTAGGTTGTGTGGTT104790.15819932662250552No Hit
TTCAAGTAATCCAGGATAGGC104340.15751997079675756No Hit
AGCAGCATTGTACAGGGCTATGT103680.15652358225232724No Hit
ACCACAGGGTAGAACCACGGAC98670.14896008739233343No Hit
TTATAAAGCAATGAGACTGATT96060.1450198236029953No Hit
GGGCTACGCCTGTCTGAGCGTCGCT95940.14483866204946252No Hit
TGTCAGTTTGTCAAATACCCCAA95530.14421969340822552No Hit
CTTCTCACTACTGCACTTGACTAG95500.14417440301984233No Hit
CCCCCACTGCTAAATTTGACTGGCTT92240.13925284748220165No Hit
TCGTTTCCCGGCCAATGCACCA90670.1368826504901477No Hit
CCCCCCACTGCTAAATTTGACTGG90550.1367014889366149No Hit
CACCCGGCTGTGTGCACATGTGC90300.1363240690334216No Hit
CGCGACCTCAGATCAGACGTGGCG89860.13565981000380137No Hit
TAGCTTATCAGACTGATGTTGACA89470.1350710349548198No Hit
AATGACACGATCACTCCCGTTG87140.13155348145705822No Hit
TTCAAGTAATCCAGGATAGGCTT86410.13045141533973376No Hit
TACCTGGTTGATCCTGCC86010.1298475434946245No Hit
TCCTGTCTGAGCGTCGCT85340.12883605815406643No Hit
TGTCAGTTTGTCAAATACCCCA84440.12747734650257053No Hit
ACTGGACTTGGAGTCAGAAGG82590.12468443921894007No Hit
CAAAGTGCTGTTCGTGCAGGT81650.12326534038293326No Hit
TGAGGTAGTAGGTTGTATAGT80780.12195191911982056No Hit
TAGCAGCACGTAAATATTGGC79300.11971759329291619No Hit
CCCATAAAGTAGAAAGCACTA77200.11654726610609244No Hit
AACATTCAACGCTGTCGGTGA76030.11478094095914777No Hit
TATTGCACTTGTCCCGGCCTG75770.11438842425982673No Hit
TTAATGCTAATCGTGATAGGGGTT74380.11228996959807196No Hit
TGTCAGTTTGTCAAATACC74350.11224467920968877No Hit
ACGCGACCTCAGATCAGA73770.11136906503428029No Hit
CCCCCCACTGCTAAATTTGACTGGCT73550.11103693551947019No Hit
CCTCGACACAAGGGTTTGT69820.10540583056382609No Hit
CAAAGTGCTGTTCGTGCAGGTAG69700.10522466901029329No Hit
CTCGACACAAGGGTTTGT69330.1046660875535672No Hit
TATTGCACTTGTCCCGGCCTGA68980.10413769968909657No Hit
CGCGACCTCAGATCAGACGT67770.10231098735764099No Hit
AGAGGTAGTAGGTTGCATAGTT67600.10205434182346954No Hit
CGCGACCTCAGATCAGAC67360.10169201871640396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)