FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8041027
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6749818.394213823681975No Hit
CCCCCCACTGCTAAATTTGACTG6457128.030218030607285No Hit
TTCAAGTAATCCAGGATAGGCT2955213.6751648763273645No Hit
AAGCTGCCAGTTGAAGAACTGT2272222.825783323448609No Hit
CCCCCACTGCTAAATTTGACTG2241772.787915026277116No Hit
AACATTCAACGCTGTCGGTGAGT2197852.7332951375489722No Hit
TAGCAGCACGTAAATATTGGCG2108582.622276980291199No Hit
GCCTGTCTGAGCGTCGCT1967602.4469511170650216No Hit
TAGCTTATCAGACTGATGTTGAC1627092.023485308530863No Hit
AACATTCAACGCTGTCGGTGAG1483581.845013081040519No Hit
TAGCTTATCAGACTGATGTTGACT1342871.6700229958188175No Hit
TATTGCACTTGTCCCGGCCTGTAA1058241.3160507979888638No Hit
TGAGGTAGTAGGTTGTATAGTT1000921.2447663712607855No Hit
TATTGCACTTGTCCCGGCCTGTAT975091.2126436088325534No Hit
TCCTGTCTGAGCGTCGCT891891.1091742385643029No Hit
TAGCTTATCAGACTGATGTTGA849071.0559223342988402No Hit
TGTAAACATCCTTGACTGGAAGCT814811.0133158363975148No Hit
TGAGGTAGTAGATTGTATAGTT620520.7716924716208514No Hit
TTCAAGTAATTCAGGATAGGTT581310.7229300436374607No Hit
AGCAGCATTGTACAGGGCTATGA545320.6781720792630096No Hit
TATTGCACTTGTCCCGGCCTGT509500.6336255306691546No Hit
TGTAAACATCCCCGACTGGAAGCT500000.6218111194005442No Hit
CCCCCACTGCTAAATTTGACTGGCTT482500.6000477302215251No Hit
ACTGGACTTGGAGTCAGAAGGC451560.5615700581530195No Hit
TATTGCACTTGTCCCGGCCTGTA435660.5417964645560822No Hit
CAACGGAATCCCAAAAGCAGCTGT420280.5226695545233214No Hit
AACCGGGCGGAAACACCA397340.4941408603652245No Hit
TTCTCACTACTGCACTTGACTAG362970.4513975640176311No Hit
CACCCGGCTGTGTGCACATGTGC327900.40778373210287694No Hit
CCCATAAAGTAGAAAGCACTAC311030.38680382493430254No Hit
CATAAAGTAGAAAGCACTACT307990.3830232133283472No Hit
CAACGGAATCCCAAAAGCAGCT301010.37434273010151564No Hit
CCCCCCACTGCTAAATTTGACTGGCT286950.3568574014239723No Hit
CTTCTCACTACTGCACTTGACTAG260300.32371486875992334No Hit
TAGCTTATCAGACTGATGTTGACA256010.31837972935546666No Hit
CTCCCACTGCTTCACTTGACTAG246950.3071125118719288No Hit
CAAAGAATTCTCCTTTTGGGCT232370.28898049963020894No Hit
CCCCCCACTGCTAAATTTGACTGG223530.2779868790392073No Hit
CTCCCACTGCTTCACTTGACTAGCCT211360.26285199639299806No Hit
TGAGGTAGTAGTTTGTACAGTT200710.24960741954976645No Hit
CATTGCACTTGTCTCGGTCTGA197900.2461128410587354No Hit
TCGTACGACTCTTAGCGG190110.23642502381847494No Hit
TGTAAACATCCTACACTCTCAGCT186290.23167438686625477No Hit
TTCAAGTAATCCAGGATAGGCTT173770.21610423643646515No Hit
ACCCCCCACTGCTAAATTTGACTG167040.20773465876933384No Hit
AACATTCAACGCTGTCGGTGAGTT166800.20743618943202152No Hit
CAAAGAATTCTCCTTTTGGGCTT166350.20687655942456107No Hit
TAGCAGCACGTAAATATTGGC155550.1934454392455093No Hit
AACGGAATCCCAAAAGCAGCTG149190.1855360018067344No Hit
TGAGGTAGTAGTTTGTGCTGTT143260.17816132193064393No Hit
GGAATACCGGGTGCTGTAGGCTTT142300.17696744458139488No Hit
AGCTCGGTCTGAGGCCCCTCAGT140990.17533829944856547No Hit
TTCACAGTGGCTAAGTTCTGC140030.17414442209931644No Hit
CCCCCCACTGCTAAATTTGACTGTA129930.16158383748742544No Hit
CCCATAAAGTAGAAAGCACT127470.15852452677997475No Hit
TGTAAACATCCCCGACTGGAAGC126700.15756693765609792No Hit
CACTAGATTGTGAGCTCCTGGAA125160.15565175940834422No Hit
CGCCTGTCTGAGCGTCGCT122830.1527541195919377No Hit
TATTGCACTTGTCCCGGCCTGTAG119210.14825220708747777No Hit
CTACGCCTGTCTGAGCGTCGCT119060.14806566375165758No Hit
AATGCACCCGGGCAAGGATTCT117350.14593906972330772No Hit
TGTCAGTTTGTCAAATACCCCAA114390.14225794789645652No Hit
CACTAGATTGTGAGCTCCTGGA111540.1387136245158734No Hit
TATTGCACTTGTCCCGGCCTGTT110990.1380296322845328No Hit
TTCAAGTAATCCAGGATAGGC110280.13714666049498406No Hit
CGCGACCTCAGATCAGACGTGGCG110190.13703473449349193No Hit
ATGCACCTGGGCAAGGATTCTGA110170.13700986204871593No Hit
TGTCAGTTTGTCAAATACCCCA110120.13694768093677587No Hit
CCCCCACTGCTAAATTTGACTGGCT105870.13166228642187125No Hit
AGCAGCATTGTACAGGGCTATGT103650.12890144505173282No Hit
TATTGCACTTGTCCCGGCCTGTAAT101910.12673754235621892No Hit
GGGAATACCGGGTGCTGTAGGCTTT98640.12267089763533937No Hit
TTATAAAGCAATGAGACTGATT98470.12245948185474317No Hit
CAACACCAGTCGATGGGCTGT97090.12074328316519767No Hit
TCGTTTCCCGGCCAATGCACCA94290.11726114089655464No Hit
GGAATACCGGGTGCTGTAGGCTT92380.11488582242044455No Hit
CAAAGTGCTGTTCGTGCAGGTAG91570.11387848840701568No Hit
CAACGGAATCCCAAAAGCAGCTGA90000.11192600149209796No Hit
ATCACATTGCCAGGGATTTCC89320.11108033836971323No Hit
CCCCCACTGCTAAATTTGACTGGCTA88250.10974966257419605No Hit
TTCACAGTGGCTAAGTTCCGC87470.1087796372279312No Hit
ATCACATTGCCAGGGATTTCCA85490.10631726519510505No Hit
ACTGGACTTGGAGTCAGAAGGCT84240.10476273739660369No Hit
TGAGGTAGTAGGTTGTGTGGTT83780.10419067116675519No Hit
GGGAATACCGGGTGCTGTAGGCTT82100.10210138580556936No Hit
TATTGCACTTGTCCCGGCCTGTTT80950.10067122023094811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)