FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416487
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT102451812.172750935158575No Hit
CAACGGAATCCCAAAAGCAGCTG5755796.838708358962593No Hit
CCCCCCACTGCTAAATTTGACTG3833924.555249714043401No Hit
TCCTGTCTGAGCGTCGCT3341443.970112470915716No Hit
TTCAAGTAATCCAGGATAGGCT2238792.6600052967467307No Hit
AACATTCAACGCTGTCGGTGAGT2178942.5888948678944077No Hit
AAGCTGCCAGTTGAAGAACTGT1912932.2728366359978933No Hit
AACATTCAACGCTGTCGGTGAG1778992.1136966052463455No Hit
TCGTACGACTCTTAGCGG1617991.922405393128986No Hit
CCCCCACTGCTAAATTTGACTG1491861.7725447683813924No Hit
TAGCAGCACGTAAATATTGGCG1446571.7187337187118568No Hit
TATTGCACTTGTCCCGGCCTGT969791.152250339126051No Hit
TATTGCACTTGTCCCGGCCTGTAT884691.051139269864018No Hit
TATTGCACTTGTCCCGGCCTGTAA857771.0191544286826557No Hit
TGAGGTAGTAGGTTGTATAGTT741370.8808544467543287No Hit
AACCGGGCGGAAACACCA634010.7532952881647651No Hit
CGCCTGTCTGAGCGTCGCT582970.6926524094910382No Hit
TATTGCACTTGTCCCGGCCTGTA529180.6287421343370458No Hit
CACCCGGCTGTGTGCACATGTGC485890.5773073730167942No Hit
TTCAAGTAATTCAGGATAGGTT356910.424060537371471No Hit
CTCCCACTGCTTCACTTGACTAG349870.41569600238199145No Hit
TGAGGTAGTAGATTGTATAGTT342170.40654729223724817No Hit
TAGCTTATCAGACTGATGTTGAC328910.39079250048149544No Hit
CAACGGAATCCCAAAAGCAGCTGT312820.3716752607115059No Hit
TAGCTTATCAGACTGATGTTGA306490.3641543080860221No Hit
ACTGGACTTGGAGTCAGAAGGC298440.35458974748015415No Hit
TTAATGCTAATCGTGATAGGGGTT289370.34381327981615134No Hit
CCCTGTCTGAGCGTCGCT289010.3433855479132802No Hit
TAGCTTATCAGACTGATGTTGACT284400.33790820326818066No Hit
AGCAGCATTGTACAGGGCTATGA272420.3236742360559697No Hit
ACCGGGTGCTGTAGGCTT272360.32360294740549117No Hit
TGTAAACATCCTTGACTGGAAGCT239970.2851189575888372No Hit
CATTGCACTTGTCTCGGTCTGA233030.27687323701682187No Hit
TTCTCACTACTGCACTTGACTAG230260.2735820776530636No Hit
TGATGTAGTAGGTTGTATAGTT222700.26459970769277014No Hit
CATAAAGTAGAAAGCACTACT220340.26179568744061504No Hit
TGTAAACATCCCCGACTGGAAGCT218260.2593243475573597No Hit
CTACGCCTGTCTGAGCGTCGCT195880.23273368092887212No Hit
ACCGGGTGCTGTAGGCTTA192620.22886033091953925No Hit
ACCGGGTGCTGTAGGCTTT187760.22308595023077918No Hit
GGAATACCGGGTGCTGTAGGCTTT182630.21699077061486577No Hit
ATCCCGGACGAGCCCCCA178200.2117272919212018No Hit
CCCATAAAGTAGAAAGCACTAC177520.21091935388244526No Hit
CCCCCACTGCTAAATTTGACTGGCTT169300.20115280876688815No Hit
AGCTCGGTCTGAGGCCCCTCAGT167950.19954881413112147No Hit
GGAATACCGGGTGCTGTAGGCTT160560.1907684286805172No Hit
ACGCCTGTCTGAGCGTCGCT147910.17573840487129605No Hit
CCCCCCACTGCTAAATTTGACTGG146190.17369479689091186No Hit
TATTGCACTTGTCCCGGCCTGTT145910.17336211652201208No Hit
TGAGGTAGTAGTTTGTACAGTT144070.17117593124067082No Hit
CCCCCCACTGCTAAATTTGACTGGCT139490.16573423092081055No Hit
TCCCACTGCTTCACTTGACTAG135250.16069649962032853No Hit
AACGGAATCCCAAAAGCAGCTG133720.1588786390331263No Hit
CCCATAAAGTAGAAAGCACT131940.15676374240226357No Hit
TACGCCTGTCTGAGCGTCGCT130210.15470825298013294No Hit
CAACGGAATCCCAAAAGCAGCT129730.15413794377630477No Hit
TGAGGTAGTAGGTTGTGTGGTT128020.15210621723766696No Hit
AACATTCAACGCTGTCGGTGAGTT125340.14892199084962646No Hit
GCCGGTCTGAGCGTCGCT124220.14759126937402742No Hit
CTTCTCACTACTGCACTTGACTAG118400.14067627027761107No Hit
TCCGGTCTGAGCGTCGCT118350.14061686306887897No Hit
ACCCCCCACTGCTAAATTTGACTG116190.1380504716516523No Hit
TGAGAACTGAATTCCATGGGTT110870.13172954464255693No Hit
TTCAAGTAATCCAGGATAGGC109910.13058892623490062No Hit
TAATGTGAATTGCAGGACA109060.12957900368645495No Hit
ATCCCACCGCTGCCACCA107660.12791560184195616No Hit
TGAGGTAGTAGTTTGTGCTGTT107120.1272740039876495No Hit
TATTGCACTTGTCCCGGCCTGTAG107030.1271670710119317No Hit
TGATGTAGTAGATTGTATAGTT104530.12419671057532673No Hit
TAGCAGCACGTAAATATTGGC103910.12346006118704869No Hit
TACCACCCTGAACGCGCCCGA103280.12271153035702424No Hit
TCGTTTCCCGGGCGGCGCACCA103130.12253330873082796No Hit
CACTAGATTGTGAGCTCCTGGA100300.11917086071659114No Hit
CAACACCAGTCGATGGGCTGT100050.11887382467293064No Hit
ACTTGACTTGGAGTCAGAAGGC98820.11741240733812101No Hit
TTCAAGTAATCCAGGATAGGCTT96680.11486977880438715No Hit
TTCACCACCTTCTCCACCCAGC95120.11301627389194566No Hit
TCCTTCTGATCGAGGCCC94310.11205387711048566No Hit
TCGTTTCCCGGCCAATGCACCA92890.11036671238249403No Hit
TGTAAACATCCTACACTCTCAGCT86920.10327349165988138No Hit
TTCACAGTGGCTAAGTTCTGC84700.10063581159217617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)