FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9508667
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT117704512.378654126808732No Hit
CAACGGAATCCCAAAAGCAGCTG6746597.095200620654819No Hit
CCCCCCACTGCTAAATTTGACTG4747764.9930868333069185No Hit
TTCAAGTAATCCAGGATAGGCT2677152.815484021051531No Hit
AACATTCAACGCTGTCGGTGAGT2487762.616307837891473No Hit
AAGCTGCCAGTTGAAGAACTGT2384762.5079856093393533No Hit
TAGCAGCACGTAAATATTGGCG2121482.2311013730946723No Hit
CCCCCACTGCTAAATTTGACTG2035732.140920488644728No Hit
AACATTCAACGCTGTCGGTGAG1732951.8224952035863704No Hit
TATTGCACTTGTCCCGGCCTGT1179931.2408994867524543No Hit
TATTGCACTTGTCCCGGCCTGTAT1064671.1196837579862666No Hit
AACCGGGCGGAAACACCA1007981.060064465397726No Hit
TGAGGTAGTAGGTTGTATAGTT992971.0442788668485288No Hit
TATTGCACTTGTCCCGGCCTGTAA953451.0027167845924145No Hit
TCGTACGACTCTTAGCGG849220.8931009993304003No Hit
GGAATACCGGGTGCTGTAGGCTTT755920.7949799903603734No Hit
GGAATACCGGGTGCTGTAGGCTT672530.7072810521180308No Hit
TATTGCACTTGTCCCGGCCTGTA610450.6419932467926367No Hit
TTCAAGTAATTCAGGATAGGTT498870.524647671434913No Hit
CACCCGGCTGTGTGCACATGTGC488620.5138680321857942No Hit
CGCCTGTCTGAGCGTCGCT485600.5106919823777613No Hit
TGAGGTAGTAGATTGTATAGTT485110.5101766630380473No Hit
TAGCTTATCAGACTGATGTTGA481970.5068744125753905No Hit
CAACGGAATCCCAAAAGCAGCT481660.5065483942176122No Hit
TAGCTTATCAGACTGATGTTGAC473400.4978615824910053No Hit
TTAATGCTAATCGTGATAGGGGTT406940.4279674532718413No Hit
ACCGGGTGCTGTAGGCTT403020.4238448985541296No Hit
CAACGGAATCCCAAAAGCAGCTGT392310.41258149012895284No Hit
ACTGGACTTGGAGTCAGAAGGC388390.4084589354112412No Hit
AGCAGCATTGTACAGGGCTATGA370990.39015984049078595No Hit
CTACGCCTGTCTGAGCGTCGCT370100.38922385230232587No Hit
TAGCTTATCAGACTGATGTTGACT356040.3744373422689006No Hit
TTCTCACTACTGCACTTGACTAG343450.36119679025461715No Hit
CTCCCACTGCTTCACTTGACTAG331320.3484400074163918No Hit
CATTGCACTTGTCTCGGTCTGA294540.30975950677418823No Hit
TGTAAACATCCTTGACTGGAAGCT284630.2993374360465037No Hit
TCCTGTCTGAGCGTCGCT282840.2974549429483649No Hit
ACCGGGTGCTGTAGGCTTT282770.29738132589983435No Hit
TGTAAACATCCCCGACTGGAAGCT277820.29217554889660136No Hit
CATAAAGTAGAAAGCACTACT241060.2535160816968351No Hit
ATCCCGGACGAGCCCCCA237820.2501086640219917No Hit
TCGTTTCCCGGCCAATGCACCA232070.24406154932126659No Hit
AGCTCGGTCTGAGGCCCCTCAGT216520.22770804782626208No Hit
ACCGGGTGCTGTAGGCTTA215030.22614105636468285No Hit
TGAGAACTGAATTCCATGGGTT206060.21670755743155168No Hit
TGAGGTAGTAGTTTGTACAGTT203460.213973209914702No Hit
CCCCCACTGCTAAATTTGACTGGCTT201550.21196451616193943No Hit
CTTCTCACTACTGCACTTGACTAG200590.2109549109249488No Hit
TACGCCTGTCTGAGCGTCGCT196060.20619083621289924No Hit
CCCATAAAGTAGAAAGCACTAC191240.20112177658550878No Hit
AACGGAATCCCAAAAGCAGCTG187620.19731472350435658No Hit
TATTGCACTTGTCCCGGCCTGTT184910.1944646920541018No Hit
ACGCCTGTCTGAGCGTCGCT181470.1908469399548854No Hit
CCCCCCACTGCTAAATTTGACTGG179910.18920633144477558No Hit
CCCATAAAGTAGAAAGCACT175080.18412675509616647No Hit
TGAGGTAGTAGTTTGTGCTGTT160320.16860407457743554No Hit
CTCGACACAAGGGTTTGT159590.16783635392847387No Hit
CACTAGATTGTGAGCTCCTGGA152490.1603694818632307No Hit
GGGAATACCGGGTGCTGTAGGCTT151420.15924419269283485No Hit
CCCCCCACTGCTAAATTTGACTGGCT151270.1590864418745551No Hit
TGAGGTAGTAGGTTGTGTGGTT150390.15816097040731367No Hit
GGGAATACCGGGTGCTGTAGGCTTT150090.1578454687707541No Hit
AACATTCAACGCTGTCGGTGAGTT148250.15591039206652205No Hit
TGATGTAGTAGGTTGTATAGTT140720.14799130098887678No Hit
GTCAGGATGGCCGAGCGGTCTAAGG135170.14215452071252468No Hit
TAGCAGCACGTAAATATTGGC132210.13904157123180358No Hit
GTCCGGCCGTGTCGGCGG129530.1362230899452047No Hit
GAATACCGGGTGCTGTAGGCTTT128770.13542381913258714No Hit
TATTGCACTTGTCCCGGCCTGTAG127460.13404612865294369No Hit
TGTAAACATCCTACACTCTCAGCT126870.13342564210104318No Hit
TATTGCACTTGTCCCGGCCTGTAC122220.1285353667343698No Hit
TTCAAGTAATCCAGGATAGGCTT117840.12392904284060005No Hit
TTCAAGTAATCCAGGATAGGC115700.12167846449980843No Hit
AATGCACCCGGGCAAGGATTCT114880.12081609335987895No Hit
TATTGCACTTGTCCCGGCCTGA114380.12029025729894632No Hit
GAATACCGGGTGCTGTAGGCTT112190.11798709535206145No Hit
GGAATACCGGGTGCTGTAGGCT111190.1169354232301962No Hit
TTCACAGTGGCTAAGTTCTGC110880.11660940487241797No Hit
CAACGGAATCCCAAAAGCAGCTGA109450.11510551373815067No Hit
GGAATACCGGGTGCTGTAGGCTTA109000.11463226128331132No Hit
CACTAGATTGTGAGCTCCTGGAA107870.11344387178560358No Hit
GGGCTACGCCTGTCTGAGCGTCGCT104130.1095106180498276No Hit
GCCTGTCTGAGCGTCGCTT103340.10867979707355406No Hit
ACCCCCCACTGCTAAATTTGACTG102850.10816447773384008No Hit
TCGTTTCCCGGGCGGCGCACCA102360.10764915839412612No Hit
TCCCACTGCTTCACTTGACTAG101760.10701815512100697No Hit
CACCCGTAGAACCGACCTTGCG100930.10614526725985882No Hit
TGAGGTAGTAGGTTGTATAGT97960.10302180105791905No Hit
TGTAAACATCCCCGACTGGAAGC97920.10297973417304446No Hit
CAACACCAGTCGATGGGCTGT97500.10253803188186104No Hit
TTCACCACCTTCTCCACCCAGC95910.10086587320809531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)