FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10770463
Sequences flagged as poor quality0
Sequence length18-26
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT365738833.957574525811935No Hit
CAACGGAATCCCAAAAGCAGCTG5811385.3956640489828525No Hit
CCCCCCACTGCTAAATTTGACTG4470454.150657218728666No Hit
AACATTCAACGCTGTCGGTGAGT2219332.0605706551333958No Hit
TTCAAGTAATCCAGGATAGGCT2022541.8778579899489931No Hit
CCCCCACTGCTAAATTTGACTG1967881.8271080825401842No Hit
TCGTACGACTCTTAGCGG1698171.576691735536346No Hit
AACATTCAACGCTGTCGGTGAG1626661.5102971896379942No Hit
AAGCTGCCAGTTGAAGAACTGT1199201.1134154585554956No Hit
TAGCAGCACGTAAATATTGGCG1054620.97917796105887No Hit
CGCCTGTCTGAGCGTCGCT1048900.9738671401591557No Hit
TGAGGTAGTAGGTTGTATAGTT747560.6940834391242048No Hit
AACCGGGCGGAAACACCA714490.6633790952162409No Hit
TATTGCACTTGTCCCGGCCTGTAA704520.6541222972494312No Hit
TATTGCACTTGTCCCGGCCTGTAT614590.5706254225096916No Hit
TATTGCACTTGTCCCGGCCTGT603550.5603751667871659No Hit
CACCCGGCTGTGTGCACATGTGC495750.46028661906178037No Hit
GCCGGTCTGAGCGTCGCT385170.35761693810191814No Hit
TATTGCACTTGTCCCGGCCTGTA375400.3485458331735599No Hit
CTACGCCTGTCTGAGCGTCGCT353470.32818459150734747No Hit
TAGCTTATCAGACTGATGTTGAC311960.2896440013767282No Hit
CTCCCACTGCTTCACTTGACTAG308450.2863850885519035No Hit
ACTGGACTTGGAGTCAGAAGGC304930.2831168910751562No Hit
TAGCTTATCAGACTGATGTTGA303220.28152921559639543No Hit
TTAATGCTAATCGTGATAGGGGTT297410.2761348328293779No Hit
TAGCTTATCAGACTGATGTTGACT292520.2715946380392375No Hit
CAACGGAATCCCAAAAGCAGCTGT287120.2665809260010456No Hit
GCCTGTCTGAGCGTCGCTT283900.2635912680819757No Hit
TGAGGTAGTAGATTGTATAGTT280200.260155946870622No Hit
ACCGGGTGCTGTAGGCTT276570.25678561822272633No Hit
AGCAGCATTGTACAGGGCTATGA252800.2347160006027596No Hit
TTCAAGTAATTCAGGATAGGTT240870.22363941085912464No Hit
TACGCCTGTCTGAGCGTCGCT235920.21904350815744877No Hit
ACGCCTGTCTGAGCGTCGCT218620.20298106033138966No Hit
TTCTCACTACTGCACTTGACTAG216530.20104056807957096No Hit
GCCTGTCTGAGCGTCGCG215460.20004711032385517No Hit
TCCTGTCTGAGCGTCGCT207830.19296292090692851No Hit
ACCGGGTGCTGTAGGCTTT202580.18808847864757533No Hit
CATTGCACTTGTCTCGGTCTGA199980.18567446914770516No Hit
GGAATACCGGGTGCTGTAGGCTTT192790.17899880441537191No Hit
AGCTCGGTCTGAGGCCCCTCAGT187230.17383653794641885No Hit
CCCCCACTGCTAAATTTGACTGGCTT183070.1699741227466266No Hit
TGTAAACATCCCCGACTGGAAGCT178500.16573103681800866No Hit
ACCGGGTGCTGTAGGCTTA170160.157987637114579No Hit
GGAATACCGGGTGCTGTAGGCTT166590.15467301637821884No Hit
TGTAAACATCCTTGACTGGAAGCT165390.15355885814750952No Hit
CATAAAGTAGAAAGCACTACT162350.15073632396304595No Hit
CCCCCCACTGCTAAATTTGACTGG160280.14881440101507243No Hit
TGATGTAGTAGGTTGTATAGTT157600.14632611429982165No Hit
AACATTCAACGCTGTCGGTGAGTT154380.14333645638075168No Hit
TGAGGTAGTAGGTTGTGTGGTT142620.13241770571980055No Hit
CCCCCCACTGCTAAATTTGACTGGCT141430.1313128321410138No Hit
AACGGAATCCCAAAAGCAGCTG140440.13039365160067862No Hit
CCCATAAAGTAGAAAGCACTAC137880.12801678070849878No Hit
CAACGGAATCCCAAAAGCAGCT130770.12141539319154618No Hit
NCCTGTCTGAGCGTCGCT129950.12065405173389482No Hit
CCCATAAAGTAGAAAGCACT128660.11945633163588233No Hit
GGGAATACCGGGTGCTGTAGGCTT126150.11712588400331536No Hit
CTTCTCACTACTGCACTTGACTAG126100.11707946074370247No Hit
GTCCGGCCGTGTCGGCGG116420.10809191768264743No Hit
ATCCCGGACGAGCCCCCA114260.10608643286737071No Hit
GGGAATACCGGGTGCTGTAGGCTTT113430.10531580675779675No Hit
TGAGGTAGTAGTTTGTACAGTT111220.10326389868290714No Hit
ACCCCCCACTGCTAAATTTGACTG110720.10279966608677826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)