FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6230780
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT65239510.470518939843808No Hit
CCCCCCACTGCTAAATTTGACTG4037656.480167812055633No Hit
CAACGGAATCCCAAAAGCAGCTG3266255.242120569174325No Hit
AACATTCAACGCTGTCGGTGAGT1983393.18321301666886No Hit
AAGCTGCCAGTTGAAGAACTGT1465392.3518564288901227No Hit
CCCCCACTGCTAAATTTGACTG1378432.2122912380151445No Hit
TTCAAGTAATCCAGGATAGGCT1270562.0391668458844636No Hit
AACATTCAACGCTGTCGGTGAG1091681.7520759840662001No Hit
TAGCAGCACGTAAATATTGGCG1077901.7299599729086887No Hit
TCGTACGACTCTTAGCGG687571.1035055001139504No Hit
CGCCTGTCTGAGCGTCGCT655021.051264849665692No Hit
TATTGCACTTGTCCCGGCCTGTAA630321.0116229428739258No Hit
TATTGCACTTGTCCCGGCCTGTAT602370.9667649957148222No Hit
TATTGCACTTGTCCCGGCCTGT537110.8620269051386825No Hit
TAGCTTATCAGACTGATGTTGAC516270.8285800493678158No Hit
TGAGGTAGTAGGTTGTATAGTT511500.820924507044062No Hit
TCCTGTCTGAGCGTCGCT427320.6858210368525289No Hit
AACCGGGCGGAAACACCA386070.619617447574782No Hit
ACTGGACTTGGAGTCAGAAGGC384760.6175149820728705No Hit
AGCAGCATTGTACAGGGCTATGA374240.600631060637673No Hit
TATTGCACTTGTCCCGGCCTGTA355870.571148395545983No Hit
TAGCTTATCAGACTGATGTTGACT346760.5565274331624612No Hit
TAGCTTATCAGACTGATGTTGA292910.47010165661442066No Hit
TGAGGTAGTAGATTGTATAGTT281000.4509868748375003No Hit
TGTAAACATCCTTGACTGGAAGCT277380.44517700833603496No Hit
CTCCCACTGCTTCACTTGACTAG269950.4332523375885523No Hit
CTACGCCTGTCTGAGCGTCGCT261140.41911285585432323No Hit
TGTAAACATCCCCGACTGGAAGCT260990.4188721155296769No Hit
TACGCCTGTCTGAGCGTCGCT256000.4108634873964416No Hit
TTCAAGTAATTCAGGATAGGTT248400.3986659776143596No Hit
CACCCGGCTGTGTGCACATGTGC219210.3518179104381795No Hit
CCCCCACTGCTAAATTTGACTGGCTT211940.3401500293703196No Hit
CCCCCCACTGCTAAATTTGACTGGCT207450.33294386898590544No Hit
GCGTTCGCGCTTTCCCCTG195240.3133476065596924No Hit
TTCTCACTACTGCACTTGACTAG194800.31264143494072977No Hit
AACATTCAACGCTGTCGGTGAGTT177080.2842019779225073No Hit
GGGCTACGCCTGTCTGAGCGTCGCT176970.2840254350177666No Hit
ACGCCTGTCTGAGCGTCGCT174040.27932297400967454No Hit
CAACGGAATCCCAAAAGCAGCTGT172140.27627359656415407No Hit
CCCCCCACTGCTAAATTTGACTGG163920.263081026773534No Hit
GGAATACCGGGTGCTGTAGGCTTT157090.2521193173246367No Hit
ACCGGGTGCTGTAGGCTT151900.24378970209187295No Hit
GTCAGGATGGCCGAGCGGTCTAAGG144130.23131935327519187No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT142950.2294255293879739No Hit
CTTCTCACTACTGCACTTGACTAG140930.22618355968273635No Hit
CATTGCACTTGTCTCGGTCTGA138130.22168974028933777No Hit
GGAATACCGGGTGCTGTAGGCTT135300.21714777283100992No Hit
AGCTCGGTCTGAGGCCCCTCAGT130210.20897865114801037No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC127940.20533544756836222No Hit
GCCTGTCTGAGCGTCGCTT110200.17686389184018694No Hit
TGAGGTAGTAGTTTGTACAGTT106980.17169599953777856No Hit
GGGAATACCGGGTGCTGTAGGCTTT103970.1668651436898751No Hit
CCTCGACACAAGGGTTTGT103290.1657737875514783No Hit
TGTAAACATCCTACACTCTCAGCT101460.1628367555907928No Hit
GGGAATACCGGGTGCTGTAGGCTT100400.1611355239632919No Hit
TGAGGTAGTAGTTTGTGCTGTT100240.16087873428366914No Hit
ACCGGGTGCTGTAGGCTTT97320.1561923226305535No Hit
TATTGCACTTGTCCCGGCCTGTT97000.15567874327130793No Hit
CTCGACACAAGGGTTTGT93930.1507515912935459No Hit
ACCGGGTGCTGTAGGCTTA93060.14935529741059708No Hit
TTCAAGTAATCCAGGATAGGCTT92880.14906640902102145No Hit
CCCATAAAGTAGAAAGCACTAC92420.14832813869210595No Hit
CAACGGAATCCCAAAAGCAGCT90980.14601703157550097No Hit
TCGTTTCCCGGCCAATGCACCA90100.14460468833757573No Hit
TGAGGTAGTAGGTTGTGTGGTT90040.1445083922077172No Hit
CGCGACCTCAGATCAGACGTGGCG87950.14115407701764468No Hit
AACGGAATCCCAAAAGCAGCTG84330.13534421051617937No Hit
TCCCACTGCTTCACTTGACTAG83610.13418865695787688No Hit
ACCCCCCACTGCTAAATTTGACTG80710.12953434401471406No Hit
TATTGCACTTGTCCCGGCCTGTAG80030.12844298787631725No Hit
GAATACCGGGTGCTGTAGGCTTT76600.12293805911940399No Hit
CATAAAGTAGAAAGCACTACT76150.12221583814546494No Hit
TAGCTTATCAGACTGATGTTGACA75170.12064300135777542No Hit
AGCAGCATTGTACAGGGCTATGT74640.11979238554402499No Hit
TCGTTTCCCGGGCGGCGCACCA73570.11807510456154767No Hit
GCTTGACTAGCTTGCTGTTT71740.11513807260086217No Hit
TGTAAACATCCCCGACTGGAAGC68830.11046771030272294No Hit
CTCCCACTGCTTCACTTGACTAGCCT68720.11029116739798227No Hit
CCCCCCACTGCTAAATTTGACTGTA67650.10857388641550496No Hit
CCCCCACTGCTAAATTTGACTGGCT67290.10799610963635371No Hit
CACTAGATTGTGAGCTCCTGGA66180.1062146312339707No Hit
CTGCGTTCGCGCTTTCCCCTG65130.10452944896144625No Hit
TAGCAGCACGTAAATATTGGC64770.10395167218229501No Hit
ACTGGACTTGGAGTCAGAAGGCT64230.10308500701356811No Hit
CAAAGAATTCTCCTTTTGGGCT64210.10305290830361527No Hit
CACTAGATTGTGAGCTCCTGGAA64090.10286031604389818No Hit
TACCTGGTTGATCCTGCC62520.1003405673125997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)