FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12397844
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8192026.607616614630738No Hit
CCCCCCACTGCTAAATTTGACTG5456024.400781297135212No Hit
AACATTCAACGCTGTCGGTGAGT5200744.194874528184094No Hit
AAGCTGCCAGTTGAAGAACTGT4955513.9970740073838646No Hit
TAGCAGCACGTAAATATTGGCG4926953.9740377439819374No Hit
TTCAAGTAATCCAGGATAGGCT4684513.7784876144594173No Hit
TAGCTTATCAGACTGATGTTGAC2866572.312152016108607No Hit
TAGCTTATCAGACTGATGTTGACT2341891.8889494012023382No Hit
AACATTCAACGCTGTCGGTGAG2170061.7503527226185458No Hit
TAGCTTATCAGACTGATGTTGA2153621.737092352509033No Hit
CCCCCACTGCTAAATTTGACTG1785631.4402746154895962No Hit
TATTGCACTTGTCCCGGCCTGTAT1639281.3222298973918367No Hit
TATTGCACTTGTCCCGGCCTGTAA1602121.2922569440299458No Hit
TATTGCACTTGTCCCGGCCTGT1461991.179229227275323No Hit
TGTAAACATCCTTGACTGGAAGCT1188060.9582795202133532No Hit
CATAAAGTAGAAAGCACTACT1056800.8524062732197631No Hit
TGAGGTAGTAGGTTGTATAGTT952720.7684561928670823No Hit
AGCAGCATTGTACAGGGCTATGA929120.7494206250699719No Hit
TTCAAGTAATTCAGGATAGGTT847980.6839737618895673No Hit
TATTGCACTTGTCCCGGCCTGTA812460.6553236191712043No Hit
ACTGGACTTGGAGTCAGAAGGC785090.633247200077691No Hit
CGCCTGTCTGAGCGTCGCT761970.614598796371369No Hit
TGTAAACATCCCCGACTGGAAGCT741410.5980152678159202No Hit
TGAGGTAGTAGATTGTATAGTT683820.5515636428398357No Hit
CCCATAAAGTAGAAAGCACTAC596380.48103525096782956No Hit
GGAATACCGGGTGCTGTAGGCTTT576570.4650566663042381No Hit
CAACGGAATCCCAAAAGCAGCTGT575900.4645162497608455No Hit
CATTGCACTTGTCTCGGTCTGA490420.3955687779262265No Hit
TGTAAACATCCTACACTCTCAGCT482140.38889019736012165No Hit
TTCTCACTACTGCACTTGACTAG438170.35342435346016615No Hit
AACATTCAACGCTGTCGGTGAGTT419820.33862339290605686No Hit
TCGTACGACTCTTAGCGG398930.32177368903819087No Hit
GGAATACCGGGTGCTGTAGGCTT391820.31603882094338337No Hit
CTTCTCACTACTGCACTTGACTAG370730.29902779870435536No Hit
CCCATAAAGTAGAAAGCACT333630.269103240853813No Hit
TAGCTTATCAGACTGATGTTGACA324570.26179551864017647No Hit
CTACGCCTGTCTGAGCGTCGCT311940.25160826350129906No Hit
TTCAAGTAATCCAGGATAGGCTT306100.24689776706337008No Hit
TACGCCTGTCTGAGCGTCGCT276350.2229016593530294No Hit
CAACACCAGTCGATGGGCTGT276080.2226838795519608No Hit
CACCCGGCTGTGTGCACATGTGC273930.22094970706196979No Hit
CTCCCACTGCTTCACTTGACTAG270300.21802177862538033No Hit
TGAGGTAGTAGTTTGTACAGTT254850.20555993445311943No Hit
GCGTTCGCGCTTTCCCCTG253050.20410806911266183No Hit
TATTGCACTTGTCCCGGCCTGTT246800.19906687001385082No Hit
CACTAGATTGTGAGCTCCTGGA243500.19640511688967854No Hit
AGCTCGGTCTGAGGCCCCTCAGT236480.19074284206189399No Hit
CAAAGAATTCTCCTTTTGGGCT235840.19022662327417575No Hit
CAAAGAATTCTCCTTTTGGGCTT235100.1896297453008765No Hit
CAACGGAATCCCAAAAGCAGCT234760.18935550406990118No Hit
CCCCCCACTGCTAAATTTGACTGGCT233820.18859730772544No Hit
TGAATACCGGGTGCTGTAGGCTTT229470.18508863315266752No Hit
TGTCAGTTTGTCAAATACCCCAA227270.18331413106988603No Hit
CCCCCACTGCTAAATTTGACTGGCTT223910.1806039824343652No Hit
TGAGGTAGTAGTTTGTGCTGTT223520.1802894116105994No Hit
TTATAAAGCAATGAGACTGATT219720.17722436255852228No Hit
TGTAAACATCCCCGACTGGAAGC216080.1742883682033747No Hit
ACGCCTGTCTGAGCGTCGCT210800.17002956320469914No Hit
TTCAAGTAATCCAGGATAGGC206890.16687578904848294No Hit
TGTAAACATCCTACACTCAGCT199200.16067309767730584No Hit
CCCCCCACTGCTAAATTTGACTGG198550.16014881297102948No Hit
TAGCAGCACGTAAATATTGGC197990.159697121531776No Hit
GCCTGTCTGAGCGTCGCT196240.15828558578410892No Hit
TAGCAGCACGTAAATATTGGCGT193370.1559706671579349No Hit
AACATTCAACGCTGTCGGTGA192690.15542218469598423No Hit
ACCGGGTGCTGTAGGCTT191910.15479304304845262No Hit
TATTGCACTTGTCCCGGCCTGTAG190180.15339763913790172No Hit
AGCAGCATTGTACAGGGCTATGT187550.15127630255712204No Hit
TTCACAGTGGCTAAGTTCCG186510.15043744702707987No Hit
ACCACAGGGTAGAACCACGGAC186010.15003415109917498No Hit
TGATGTAGTAGGTTGTATAGTT184150.14853389024736882No Hit
ACTGGACTTGGAGTCAGAAGGCA182790.14743692532346753No Hit
ACTTGACTTGGAGTCAGAAGGC179800.1450252156745963No Hit
CACTAGATTGTGAGCTCCTGGAA179420.1447187107693886No Hit
AACGGAATCCCAAAAGCAGCTG171380.13823371224867806No Hit
CAAAGTGCTGTTCGTGCAGGTAG162850.13135348371862077No Hit
CAAAGTGCTGTTCGTGCAGGT162030.13069207839685673No Hit
AACATTCAACGCTGTCGGTGAGA161010.12986935470393077No Hit
TGTCAGTTTGTCAAATACCCCA157300.12687689891887655No Hit
AGCAGCATTGTACAGGGCTATG156820.12648973482808787No Hit
ACTGGACTTGGAGTCAGAAGG156380.12613483441153156No Hit
TGAATACCGGGTGCTGTAGGCTT154770.12483622152367783No Hit
TTCACAGTGGCTAAGTTCCGC154260.12442485967721485No Hit
TTCACAGTGGCTAAGTTCTGC150910.12172277696025212No Hit
TGCAAGTAATCCAGGATAGGCT149080.12024671386412025No Hit
ACCGGGTGCTGTAGGCTTT148460.11974662691351819No Hit
ACTGGACTTGGAGTCAGAAGGCT147430.11891583730203412No Hit
CGCGACCTCAGATCAGACGTGGCG143000.11534263538079685No Hit
TCGTTTCCCGGCCAATGCACCA140990.11372138575061921No Hit
AACCGGGCGGAAACACCA140060.11297125532471614No Hit
ATCCCGGACGAGCCCCCA138640.11182589488946627No Hit
TGATGTAGTAGATTGTATAGTT133050.10731704641548966No Hit
CTCCCACTGCTTCACTTGACTAGCCT130430.10520377575326807No Hit
TATTGCACTTGTCCCGGCCTGTTT126710.10220325404965572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)