FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7982880
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6872678.609261319223139No Hit
CCCCCCACTGCTAAATTTGACTG4642145.8151193554206No Hit
GCCTGTCTGAGCGTCGCT3076303.853621750546169No Hit
TTCAAGTAATCCAGGATAGGCT2355712.950952538432245No Hit
TAGCAGCACGTAAATATTGGCG2165272.7123920189204895No Hit
AACATTCAACGCTGTCGGTGAGT2109742.6428306576072798No Hit
AAGCTGCCAGTTGAAGAACTGT2036112.5505957749584107No Hit
TATTGCACTTGTCCCGGCCTGTAT1874742.3484506844647544No Hit
AACATTCAACGCTGTCGGTGAG1771412.2190111839336177No Hit
CCCCCACTGCTAAATTTGACTG1649012.0656830617521496No Hit
TATTGCACTTGTCCCGGCCTGTAA1553921.946565650492053No Hit
TAGCTTATCAGACTGATGTTGACT1193211.4947111819293288No Hit
TATTGCACTTGTCCCGGCCTGT1172961.4693443970096005No Hit
TCCTGTCTGAGCGTCGCT1076621.3486611348285331No Hit
TAGCTTATCAGACTGATGTTGAC1060051.327904215020143No Hit
TAGCTTATCAGACTGATGTTGA944491.1831444290782274No Hit
TATTGCACTTGTCCCGGCCTGTA831661.041804461547712No Hit
GGAATACCGGGTGCTGTAGGCTTT758130.9496948469725212No Hit
TTCAAGTAATTCAGGATAGGTT695730.8715275689976549No Hit
TGAGGTAGTAGGTTGTATAGTT595720.7462469685126171No Hit
TGTAAACATCCTTGACTGGAAGCT568330.7119360431323031No Hit
TGAGGTAGTAGATTGTATAGTT534980.6701591405608001No Hit
GGAATACCGGGTGCTGTAGGCTT517640.6484376565850921No Hit
AGCAGCATTGTACAGGGCTATGA492010.6163314493015052No Hit
TTAATGCTAATCGTGATAGGGGTT489050.6126235143206462No Hit
TGTAAACATCCCCGACTGGAAGCT438370.5491376545808029No Hit
CATAAAGTAGAAAGCACTACT433210.5426738219790351No Hit
CAACGGAATCCCAAAAGCAGCTGT369260.46256488886216507No Hit
ATCCCGGACGAGCCCCCA316330.39626049746457415No Hit
CATTGCACTTGTCTCGGTCTGA313250.39240224079530195No Hit
ACTGGACTTGGAGTCAGAAGGC304500.38144128434850577No Hit
TGAATACCGGGTGCTGTAGGCTTT302400.37881065480127474No Hit
CACCCGGCTGTGTGCACATGTGC298080.3733990740183994No Hit
CCCATAAAGTAGAAAGCACTAC286880.3593690497665003No Hit
TGAGAACTGAATTCCATGGGTT270670.33906309502334997No Hit
TCGTACGACTCTTAGCGG232780.2915990219068807No Hit
CCCATAAAGTAGAAAGCACT232220.29089752069428576No Hit
TATTGCACTTGTCCCGGCCTGTT231960.29057182370272383No Hit
CAACGGAATCCCAAAAGCAGCT231130.28953209869119917No Hit
CACTAGATTGTGAGCTCCTGGA229560.2875653899344598No Hit
TTCTCACTACTGCACTTGACTAG227970.28557362756298477No Hit
TATTGCACTTGTCCCGGCCTGTAG221510.27748131000340726No Hit
TGAGAACTGAATTCCATAGGCTGT213820.26784819513759445No Hit
ACCGGGTGCTGTAGGCTTA209570.26252430200629345No Hit
CACTAGATTGTGAGCTCCTGGAA208240.2608582366263804No Hit
TGAATACCGGGTGCTGTAGGCTT207660.26013168179905No Hit
AACCGGGCGGAAACACCA203460.2548704227045878No Hit
CAACACCAGTCGATGGGCTGT199530.24994738740905537No Hit
AGCTCGGTCTGAGGCCCCTCAGT192550.24120367586635402No Hit
ACCGGGTGCTGTAGGCTT181910.22787515282704987No Hit
TATTGCACTTGTCCCGGCCTGTTT174840.2190187000180386No Hit
TGTAAACATCCTACACTCTCAGCT172800.21646323131501413No Hit
CTCCCACTGCTTCACTTGACTAG170300.21333152947307235No Hit
TGAGGTAGTAGTTTGTACAGTT170080.2130559397109815No Hit
TGATGTAGTAGGTTGTATAGTT166410.208458601407011No Hit
ACCGGGTGCTGTAGGCTTT159980.2004038642695368No Hit
CCCCCACTGCTAAATTTGACTGGCTT153930.19282514581203775No Hit
TAGCTTATCAGACTGATGTTGACA151840.19020704307217445No Hit
TGATGTAGTAGATTGTATAGTT149990.18788958370913755No Hit
CAAAGAATTCTCCTTTTGGGCT147180.18436955083879503No Hit
TAGCAGCACGTAAATATTGGC146580.183617942396729No Hit
AACGGAATCCCAAAAGCAGCTG144840.18143827791473754No Hit
CTTCTCACTACTGCACTTGACTAG144580.18111258092317561No Hit
CGCCTGTCTGAGCGTCGCT134970.1690743190427515No Hit
TGTAAACATCCCCGACTGGAAGC134220.16813480849016896No Hit
AACATTCAACGCTGTCGGTGAGTT130860.16392580121459924No Hit
TGAGGTAGTAGTTTGTGCTGTT127450.1596541599021907No Hit
TTCAAGTAATCCAGGATAGGCTT125400.15708616439179846No Hit
TATTGCACTTGTCCCGGCCTGTAAT120560.15102318962579922No Hit
TATTGCACTTGTCCCGGCCTGA119590.1498080893111258No Hit
GGAATACCGGGTGCTGTAGGCTTA119540.14974545527428698No Hit
CAAAGAATTCTCCTTTTGGGCTT113550.14224189766099452No Hit
CCCCCCACTGCTAAATTTGACTGG108800.1362916641613052No Hit
AGCAGCATTGTACAGGGCTATGT107840.13508909065399957No Hit
TTCAAGTAATCCAGGATAGGC107540.13471328643296657No Hit
AGCTACATCTGGCTACTGGGTCTC104670.13111809271841743No Hit
ACCCCCCACTGCTAAATTTGACTG103510.12966498306375643No Hit
TATTGCACTTGTCCCGGCCTGTAAA101130.1266836029102279No Hit
ACTGGACTTGGAGTCAGAAGGCA99630.12480458180506282No Hit
CCCCCCACTGCTAAATTTGACTGGCT99010.12402791974826129No Hit
TTCACAGTGGCTAAGTTCCGC96550.1209463251357906No Hit
GGAATACCGGGTGCTGTAGGCT96300.12063315495159642No Hit
TTATAAAGCAATGAGACTGATT95640.1198063856653238No Hit
ACTGGACTTGGAGTCAGAAGGCT95190.11924267933377429No Hit
TTCACAGTGGCTAAGTTCTGC94290.11811526667067525No Hit
CCCCCCACTGCTAAATTTGACTGT89250.11180175575732065No Hit
TTAATGCTAATCGTGATAGGGGTTT89220.11176417533521736No Hit
TGAGAACTGAATTCCATGGGTTG87560.10968472531216805No Hit
CAAAGTGCTGTTCGTGCAGGTAG82580.10344637524302006No Hit
ACTTGACTTGGAGTCAGAAGGC82310.10310815144409036No Hit
TGTCAGTTTGTCAAATACCCCA82290.1030830978293548No Hit
CCCTGTCTGAGCGTCGCT80230.10050257551159482No Hit
TTCACCACCTTCTCCACCCAGC79850.10002655683161966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)