FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12195953
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10451218.569408229106818No Hit
CCCCCCACTGCTAAATTTGACTG7695406.30979801250464No Hit
AACATTCAACGCTGTCGGTGAGT4866963.9906352541699692No Hit
TTCAAGTAATCCAGGATAGGCT3934353.225947164604521No Hit
AAGCTGCCAGTTGAAGAACTGT3873093.175717387562907No Hit
TAGCAGCACGTAAATATTGGCG3749553.07442149047311No Hit
AACATTCAACGCTGTCGGTGAG3413852.7991662480168626No Hit
CCCCCACTGCTAAATTTGACTG2696412.210905535631369No Hit
TATTGCACTTGTCCCGGCCTGTAA1963021.6095667144666759No Hit
TATTGCACTTGTCCCGGCCTGTAT1883841.5446435387214104No Hit
TAGCTTATCAGACTGATGTTGACT1675641.373931172086347No Hit
TAGCTTATCAGACTGATGTTGAC1613771.3232012291290398No Hit
TAGCTTATCAGACTGATGTTGA1556341.276111838082682No Hit
TATTGCACTTGTCCCGGCCTGT1299491.0655091898107512No Hit
TTAATGCTAATCGTGATAGGGGTT1223441.0031524391738802No Hit
TTCAAGTAATTCAGGATAGGTT1037880.8510036075081627No Hit
TGAGGTAGTAGGTTGTATAGTT986930.8092274543858934No Hit
TATTGCACTTGTCCCGGCCTGTA948800.7779629849344286No Hit
TGTAAACATCCTTGACTGGAAGCT872510.7154094477077765No Hit
TGAGGTAGTAGATTGTATAGTT806470.6612603377530235No Hit
CATAAAGTAGAAAGCACTACT754670.6187872321252795No Hit
AGCAGCATTGTACAGGGCTATGA674840.5533310926993569No Hit
GGAATACCGGGTGCTGTAGGCTTT657280.5389328738803765No Hit
TGTAAACATCCCCGACTGGAAGCT638220.5233047388752645No Hit
TCGTACGACTCTTAGCGG589060.4829962857351123No Hit
CAACGGAATCCCAAAAGCAGCTGT528050.4329714947245205No Hit
TTCTCACTACTGCACTTGACTAG506070.4149491228770725No Hit
GGAATACCGGGTGCTGTAGGCTT448300.3675809508285248No Hit
TGAGAACTGAATTCCATGGGTT425510.3488944242405657No Hit
CATTGCACTTGTCTCGGTCTGA408690.3351029640734103No Hit
ACTGGACTTGGAGTCAGAAGGC397700.3260917781496862No Hit
CCCATAAAGTAGAAAGCACTAC359250.29456492657851335No Hit
CACCCGGCTGTGTGCACATGTGC353580.28991584339493603No Hit
TGATGTAGTAGGTTGTATAGTT345800.28353667810953354No Hit
CAACACCAGTCGATGGGCTGT326690.2678675458982172No Hit
CCCATAAAGTAGAAAGCACT325570.266949208479239No Hit
CTTCTCACTACTGCACTTGACTAG324450.26603087106026074No Hit
AACATTCAACGCTGTCGGTGAGTT314070.2575198510522302No Hit
ACCGGGTGCTGTAGGCTT311970.255797968391646No Hit
CGCCTGTCTGAGCGTCGCT304840.24995176678690054No Hit
CAACGGAATCCCAAAAGCAGCT300040.24601603499127947No Hit
CAAAGAATTCTCCTTTTGGGCT294920.24181792107595035No Hit
TGATGTAGTAGATTGTATAGTT285230.23387266251354036No Hit
ACCGGGTGCTGTAGGCTTT281300.23065028210587563No Hit
TGAGGTAGTAGTTTGTACAGTT278430.22829704246974386No Hit
TGAATACCGGGTGCTGTAGGCTTT269750.22117992747266246No Hit
ACCGGGTGCTGTAGGCTTA257570.211193008041274No Hit
TGTAAACATCCTACACTCTCAGCT245050.20092730760769575No Hit
TATTGCACTTGTCCCGGCCTGTAG239250.196171631687987No Hit
TAGCTTATCAGACTGATGTTGACA236390.1938265914930961No Hit
TATTGCACTTGTCCCGGCCTGTT235060.19273606580805944No Hit
TAGCAGCACGTAAATATTGGC231160.18953828372411735No Hit
ATCCCGGACGAGCCCCCA229540.18820997424309524No Hit
CCCCCACTGCTAAATTTGACTGGCTT228220.18712764799929943No Hit
CTCCCACTGCTTCACTTGACTAG227400.1864552938175475No Hit
TTCACAGTGGCTAAGTTCCG223190.1830033290551382No Hit
TCGTTTCCCGGCCAATGCACCA221860.18191280337010154No Hit
TGAGGTAGTAGTTTGTGCTGTT208010.17055657725148662No Hit
GCCTGTCTGAGCGTCGCT203160.16657984824966116No Hit
AACGGAATCCCAAAAGCAGCTG201360.16510394882630328No Hit
CCCCCCACTGCTAAATTTGACTGG198840.16303768963360224No Hit
TGTCAGTTTGTCAAATACCCCAA197140.1616437846226531No Hit
CACTAGATTGTGAGCTCCTGGA195630.16040566899528064No Hit
CAAAGAATTCTCCTTTTGGGCTT192270.1576506567383459No Hit
TGAGAACTGAATTCCATAGGCTGT191870.15732267908871084No Hit
TGTAAACATCCCCGACTGGAAGC189520.15539581039710468No Hit
AACATTCAACGCTGTCGGTGA189240.1551662260423601No Hit
AGCTCGGTCTGAGGCCCCTCAGT183940.1508205221846952No Hit
TTAATGCTAATCGTGATAGGGGTTT182560.14968899929345414No Hit
TGAATACCGGGTGCTGTAGGCTT180460.14796711663286993No Hit
ACCCCCCACTGCTAAATTTGACTG179920.14752434680586257No Hit
CACTAGATTGTGAGCTCCTGGAA174180.14281786753359904No Hit
AACCGGGCGGAAACACCA173780.14248988988396397No Hit
TTCAAGTAATCCAGGATAGGCTT172970.1418257351434529No Hit
TTCAAGTAATCCAGGATAGGC169150.13869354858943783No Hit
CTACGCCTGTCTGAGCGTCGCT167430.13728324469600695No Hit
TTCACAGTGGCTAAGTTCTG162480.13322452128177273No Hit
TGTCAGTTTGTCAAATACCCCA159440.13073189114454606No Hit
ACTTGACTTGGAGTCAGAAGGC157080.12879682301169904No Hit
TTAATGCTAATCGTGATAGGGGT149950.12295062140695359No Hit
AGCTACATCTGGCTACTGGGTCTC147970.12132713204125992No Hit
CCCCCCACTGCTAAATTTGACTGGCT146350.1199988225602378No Hit
TGATAACTGAATTCCATGGGTT145820.11956425217447132No Hit
TATTGCACTTGTCCCGGCCTGTAAT142600.11692403209490887No Hit
AACATTCAACGCTGTCGGTGAGA141670.11616148405950727No Hit
TCGTTTCCCGGGCGGCGCACCA138810.1138164438646164No Hit
TATTGCACTTGTCCCGGCCTGTTT138260.11336547459636817No Hit
AGCTACATTGTCTGCTGGGTTT134280.11010209698249904No Hit
CAAAGTGCTGTTCGTGCAGGT134030.1098971109514771No Hit
TTATAAAGCAATGAGACTGATT132130.10833921711571044No Hit
ACTGGACTTGGAGTCAGAAGGCT127850.104829856264615No Hit
TATTGCACTTGTCCCGGCCTGTAAA127390.10445268196753463No Hit
TTCACAGTGGCTAAGTTCTGC126710.103895119963155No Hit
ACTGGACTTGGAGTCAGAAGGCA126490.10371473225585569No Hit
AGCAGCATTGTACAGGGCTATG125080.102558611040892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)