FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7058623
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5986988.481795953686717No Hit
CCCCCCACTGCTAAATTTGACTG4838596.854863901925347No Hit
AACATTCAACGCTGTCGGTGAGT2843454.028335271624508No Hit
TAGCAGCACGTAAATATTGGCG2591063.670772613865339No Hit
TTCAAGTAATCCAGGATAGGCT2348623.3273061898900114No Hit
AAGCTGCCAGTTGAAGAACTGT1898552.689688909579106No Hit
AACATTCAACGCTGTCGGTGAG1620992.2964677388210135No Hit
CCCCCACTGCTAAATTTGACTG1422102.0146989009046097No Hit
TATTGCACTTGTCCCGGCCTGTAA1224531.7348001161132987No Hit
TATTGCACTTGTCCCGGCCTGTAT1156971.6390873970744717No Hit
TAGCTTATCAGACTGATGTTGA1047551.4840713266596048No Hit
TAGCTTATCAGACTGATGTTGACT978401.3861060436291894No Hit
TAGCTTATCAGACTGATGTTGAC947571.3424289695029754No Hit
TATTGCACTTGTCCCGGCCTGT678940.9618589914775162No Hit
GGAATACCGGGTGCTGTAGGCTTT634410.8987730326439024No Hit
TTCAAGTAATTCAGGATAGGTT607860.8611594641051095No Hit
TTCTCACTACTGCACTTGACTAG513240.7271106560018858No Hit
TGTAAACATCCTTGACTGGAAGCT512520.7260906270245627No Hit
TATTGCACTTGTCCCGGCCTGTA476150.6745649966006118No Hit
TGAGGTAGTAGGTTGTATAGTT438410.6210984777059207No Hit
CATAAAGTAGAAAGCACTACT435580.6170891971422755No Hit
GGAATACCGGGTGCTGTAGGCTT416490.5900442621740813No Hit
AGCAGCATTGTACAGGGCTATGA411210.5825640496737111No Hit
TGAGGTAGTAGATTGTATAGTT387510.5489880958368226No Hit
CTTCTCACTACTGCACTTGACTAG378290.5359260580994338No Hit
TGTAAACATCCCCGACTGGAAGCT366440.5191380811809895No Hit
CAACGGAATCCCAAAAGCAGCTGT349780.4955357440112611No Hit
TGAATACCGGGTGCTGTAGGCTTT344670.4882963716860923No Hit
CCCCCACTGCTAAATTTGACTGGCTT318210.45081030676946476No Hit
CTCCCACTGCTTCACTTGACTAG272840.3865343141289739No Hit
ACTGGACTTGGAGTCAGAAGGC252540.3577751637961115No Hit
CCCATAAAGTAGAAAGCACTAC242800.3439764384639894No Hit
CCCCCCACTGCTAAATTTGACTGGCT239270.3389754630612798No Hit
TGAATACCGGGTGCTGTAGGCTT225200.319042396796089No Hit
CATTGCACTTGTCTCGGTCTGA220280.3120721987843805No Hit
CAACGGAATCCCAAAAGCAGCT212040.30039853382168163No Hit
CACCCGGCTGTGTGCACATGTGC204010.2890223773107021No Hit
CAAAGAATTCTCCTTTTGGGCT181900.25769898746540226No Hit
AACATTCAACGCTGTCGGTGAGTT181560.2572173071149996No Hit
CCCATAAAGTAGAAAGCACT176070.24943958616291026No Hit
CCCCCCACTGCTAAATTTGACTGG166720.23619337652683817No Hit
TAGCTTATCAGACTGATGTTGACA156210.22130378687174537No Hit
TGTAAACATCCTACACTCTCAGCT153970.2181303633867399No Hit
TGATGTAGTAGGTTGTATAGTT144170.2042466356398408No Hit
TAGCAGCACGTAAATATTGGC140770.1994298321358146No Hit
TGTAAACATCCCCGACTGGAAGC139030.19696476210728353No Hit
CACTAGATTGTGAGCTCCTGGA136230.19299798275102664No Hit
CAAAGAATTCTCCTTTTGGGCTT135910.192544636538883No Hit
TATTGCACTTGTCCCGGCCTGTAG135530.19200628791196242No Hit
CTCCCACTGCTTCACTTGACTAGCCT128440.18196183589915482No Hit
TATTGCACTTGTCCCGGCCTGTT128370.18186266641524843No Hit
TGATGTAGTAGATTGTATAGTT126340.17898675138196218No Hit
TGAGGTAGTAGTTTGTACAGTT121980.17280990924150502No Hit
TTCAAGTAATCCAGGATAGGCTT120140.17020316852167908No Hit
AGCTCGGTCTGAGGCCCCTCAGT116730.1653721979485234No Hit
CACTAGATTGTGAGCTCCTGGAA116050.16440883724771815No Hit
TGTCAGTTTGTCAAATACCCCAA114360.16201460256483452No Hit
AACATTCAACGCTGTCGGTGA112280.15906785218590086No Hit
ACCCCCCACTGCTAAATTTGACTG110530.1565886150882403No Hit
AACGGAATCCCAAAAGCAGCTG109230.15474689610140677No Hit
TTCAAGTAATCCAGGATAGGC107500.15229599314200518No Hit
CAACACCAGTCGATGGGCTGT106210.1504684412243011No Hit
TGAGGTAGTAGTTTGTGCTGTT101610.14395158942473624No Hit
GCCTGTCTGAGCGTCGCT101250.14344157493607465No Hit
TATTGCACTTGTCCCGGCCTGTTT100570.1424782142352694No Hit
TTCACAGTGGCTAAGTTCCG98250.139191454197228No Hit
CGCCTGTCTGAGCGTCGCT98070.1389364469528972No Hit
CAACGGAATCCCAAAAGCAGCTGA96110.13615970140351735No Hit
AACATTCAACGCTGTCGGTGAGA94810.13431798241668383No Hit
CCCCCACTGCTAAATTTGACTGGCT93470.13241959515333232No Hit
AACCGGGCGGAAACACCA93230.13207958549422458No Hit
GGAATACCGGGTGCTGTAGGCTTA91150.12913283511529092No Hit
TGTCAGTTTGTCAAATACCCCA90180.12775862940973048No Hit
ACTTGACTTGGAGTCAGAAGGC89420.12668193215588933No Hit
ACCGGGTGCTGTAGGCTT87670.12420269505822878No Hit
CTACGCCTGTCTGAGCGTCGCT87250.12360767815479023No Hit
TTCACAGTGGCTAAGTTCTGC86840.12302682832048122No Hit
TATTGCACTTGTCCCGGCCTGTAAT85000.12042008760065526No Hit
TTAATGCTAATCGTGATAGGGGTT84120.11917338551726024No Hit
CAAAGTGCTGTTCGTGCAGGT82210.11646747531352786No Hit
CCCCCACTGCTAAATTTGACTGGCTA81540.1155182816818521No Hit
TATTGCACTTGTCCCGGCCTGTAAA81490.11544744633620466No Hit
ACCGGGTGCTGTAGGCTTA81340.11523494029926235No Hit
TATTGCACTTGTCCCGGCCTGA79910.11320904941374543No Hit
ACCGGGTGCTGTAGGCTTT79270.11230235698945815No Hit
CTACGGGGATGATTTTACGAACTGAA76830.10884559212186286No Hit
TCGTTTCCCGGCCAATGCACCA76790.10878892384534491No Hit
CCCATAAAGTAGAAAGCACTA76310.10810890452712944No Hit
CCCCCCACTGCTAAATTTGACTGTA75200.10653635985375617No Hit
TTCACAGTGGCTAAGTTCTG71860.1018045587645069No Hit
ACTGGACTTGGAGTCAGAAGGCT71860.1018045587645069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)