FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6266009
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4650107.421151166555936No Hit
CCCCCCACTGCTAAATTTGACTG2579504.1166554341048665No Hit
TTCAAGTAATCCAGGATAGGCT2476023.9515104430906502No Hit
TAGCAGCACGTAAATATTGGCG2173033.46796501569021No Hit
AAGCTGCCAGTTGAAGAACTGT2126473.3936593452068133No Hit
AACATTCAACGCTGTCGGTGAGT1794092.8632100592258967No Hit
TAGCTTATCAGACTGATGTTGAC1765512.817598889500478No Hit
TAGCTTATCAGACTGATGTTGACT1111821.7743670652244514No Hit
TAGCTTATCAGACTGATGTTGA1058401.6891134372772207No Hit
AACATTCAACGCTGTCGGTGAG968471.5455930561223261No Hit
TGTAAACATCCTTGACTGGAAGCT828771.3226441264288002No Hit
TGAGGTAGTAGGTTGTATAGTT825591.3175691257385682No Hit
CCCCCACTGCTAAATTTGACTG781361.2469819306036745No Hit
TATTGCACTTGTCCCGGCCTGT620820.990774191355295No Hit
AGCAGCATTGTACAGGGCTATGA606100.9672823642608877No Hit
TATTGCACTTGTCCCGGCCTGTAT595840.9509083054301389No Hit
TATTGCACTTGTCCCGGCCTGTAA595620.9505572047534563No Hit
TGAGGTAGTAGATTGTATAGTT582900.9302572019925283No Hit
CATAAAGTAGAAAGCACTACT574520.9168834580352501No Hit
TTCAAGTAATTCAGGATAGGTT561680.8963919458143134No Hit
ACTGGACTTGGAGTCAGAAGGC457490.7301138571617117No Hit
CCCCCACTGCTAAATTTGACTGGCTT417960.6670274492104943No Hit
TGTAAACATCCCCGACTGGAAGCT396470.6327312967472597No Hit
CCCATAAAGTAGAAAGCACTAC335780.5358753873478317No Hit
TATTGCACTTGTCCCGGCCTGTA331160.5285022731374946No Hit
CAACGGAATCCCAAAAGCAGCTGT322610.5148572241118708No Hit
CCCCCCACTGCTAAATTTGACTGGCT304370.4857477861905401No Hit
TTCTCACTACTGCACTTGACTAG297830.4753105206200629No Hit
TAGCTTATCAGACTGATGTTGACA262430.41881522991748016No Hit
CGCCTGTCTGAGCGTCGCT249850.3987386548598957No Hit
CATTGCACTTGTCTCGGTCTGA232900.371687943633659No Hit
CTCCCACTGCTTCACTTGACTAG232850.371608148025322No Hit
CTTCTCACTACTGCACTTGACTAG232580.3711772517403023No Hit
TGTAAACATCCTACACTCTCAGCT223100.35604800439961065No Hit
TGAGGTAGTAGTTTGTACAGTT220550.3519784283744246No Hit
TGAGGTAGTAGTTTGTGCTGTT179210.2860034194014085No Hit
AACATTCAACGCTGTCGGTGAGTT179120.2858597873064019No Hit
CACCCGGCTGTGTGCACATGTGC178340.284614975816345No Hit
CAAAGAATTCTCCTTTTGGGCT177120.2826679629729227No Hit
GCCTGTCTGAGCGTCGCT174720.27883777377274754No Hit
TTCAAGTAATCCAGGATAGGCTT170250.2717040463874214No Hit
CTCCCACTGCTTCACTTGACTAGCCT168090.26825687610726384No Hit
TCGTACGACTCTTAGCGG160060.2554417014083446No Hit
CTACGCCTGTCTGAGCGTCGCT157410.2512125341664846No Hit
TAGCAGCACGTAAATATTGGC156190.24926552132306226No Hit
CCCCCCACTGCTAAATTTGACTGG153270.24460545779618256No Hit
AGCTCGGTCTGAGGCCCCTCAGT150310.23988155778263326No Hit
CAACGGAATCCCAAAAGCAGCT148530.2370408341258367No Hit
TACGCCTGTCTGAGCGTCGCT144720.23096040877055873No Hit
GTCAGGATGGCCGAGCGGTCTAAGG144520.23064122633721082No Hit
CCCATAAAGTAGAAAGCACT137060.21873572157333318No Hit
AGCAGCATTGTACAGGGCTATGT136080.21717172764992837No Hit
TAGCAGCACGTAAATATTGGCGT133950.21377243473477298No Hit
TTATAAAGCAATGAGACTGATT129010.2058886286310792No Hit
CAAAGAATTCTCCTTTTGGGCTT128690.20537793673772253No Hit
TGTCAGTTTGTCAAATACCCCA116570.18603548127683825No Hit
TATTGCACTTGTCCCGGCCTGTT116350.18568438060015555No Hit
AACGGAATCCCAAAAGCAGCTG110030.17559821570636108No Hit
TGTAAACATCCTACACTCAGCT108510.17317242921291687No Hit
TGTCAGTTTGTCAAATACCCCAA106580.1700923187311094No Hit
TGTAAACATCCCCGACTGGAAGC105410.16822510149602402No Hit
GCGTTCGCGCTTTCCCCTG104720.16712392210097368No Hit
TTCAAGTAATCCAGGATAGGC103640.1654003369608949No Hit
TTCACAGTGGCTAAGTTCCGC97560.15569719098711796No Hit
AGCAGCATTGTACAGGGCTATG97500.15560143625711356No Hit
TGAGGTAGTAGGTTGTGTGGTT93110.1485953818451266No Hit
CCCCCACTGCTAAATTTGACTGGCT92700.14794105785676337No Hit
CAACACCAGTCGATGGGCTGT92450.14754207981507847No Hit
TTCACAGTGGCTAAGTTCCG87190.13914758181802803No Hit
TCGTTTCCCGGCCAATGCACCA84620.1350460875495072No Hit
ATCACATTGCCAGGGATTTCC83910.13391298991112205No Hit
ATCACATTGCCAGGGATTTCCA83610.1334342162611002No Hit
CAAAGTGCTGTTCGTGCAGGTAG83270.1328916061244087No Hit
TGAGGTAGTAGGTTGTATAGT80560.1285666841525443No Hit
TGTAAACATCCTTGACTGGAAGC79180.12636432536244363No Hit
ACTGGACTTGGAGTCAGAAGGCT78210.12481629056070619No Hit
ACCCCCCACTGCTAAATTTGACTG77010.12290119596061863No Hit
CACTAGATTGTGAGCTCCTGGA75610.12066691892718316No Hit
TTCACAGTGGCTAAGTTCTGC74930.11958169865380021No Hit
GGGCTACGCCTGTCTGAGCGTCGCT74840.11943806655879365No Hit
ACGCCTGTCTGAGCGTCGCT73650.1175389310803735No Hit
AACATTCATTGCTGTCGGTGGGTT72890.11632603783365138No Hit
TCCCACTGCTTCACTTGACTAG72340.11544828614194458No Hit
CACTAGATTGTGAGCTCCTGGAA71010.11332572296018088No Hit
ACCGGGTGCTGTAGGCTT70450.11243201214680668No Hit
CGCGACCTCAGATCAGACGTGGCG69260.11053287666838653No Hit
TTCACCACCTTCTCCACCCAGC68950.11003814389669725No Hit
TAGCAGCACGTAAATATTGGCGA68590.10946361551667097No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC67960.10845819085162502No Hit
AGAGGTAGTAGGTTGCATAGTT67640.10794749895826833No Hit
ACTGGACTTGGAGTCAGAAGGCA64790.10339914928306039No Hit
ACCACAGGGTAGAACCACGGAC64710.10327147630972122No Hit
CCCCCCACTGCTAAATTTGACTGTA64540.10300017124137549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)