FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8405505
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7130268.482845468535203No Hit
CCCCCCACTGCTAAATTTGACTG6666397.930980946415474No Hit
AACATTCAACGCTGTCGGTGAGT3459974.116314248816698No Hit
AACATTCAACGCTGTCGGTGAG2527653.007136394541434No Hit
CCCCCACTGCTAAATTTGACTG2300912.7373846068737095No Hit
TTCAAGTAATCCAGGATAGGCT2020522.403805601210159No Hit
TATTGCACTTGTCCCGGCCTGTAA2013612.3955847982958787No Hit
TATTGCACTTGTCCCGGCCTGTAT1901072.2616963525689417No Hit
TAGCAGCACGTAAATATTGGCG1841842.1912306280229443No Hit
TAGCTTATCAGACTGATGTTGACT1686292.0061733352130537No Hit
AAGCTGCCAGTTGAAGAACTGT1494981.7785724950493753No Hit
TAGCTTATCAGACTGATGTTGAC1389361.6529167492018624No Hit
TAGCTTATCAGACTGATGTTGA1221831.453606892149847No Hit
GGAATACCGGGTGCTGTAGGCTTT940961.1194568321594003No Hit
TGAGGTAGTAGGTTGTATAGTT933581.1106768718833668No Hit
TATTGCACTTGTCCCGGCCTGT783960.9326744794036765No Hit
TATTGCACTTGTCCCGGCCTGTA761980.9065249500178751No Hit
GGAATACCGGGTGCTGTAGGCTT644860.767187694255134No Hit
CACCCGGCTGTGTGCACATGTGC627260.7462490356022631No Hit
TGAGGTAGTAGATTGTATAGTT579650.6896075845532184No Hit
AGCAGCATTGTACAGGGCTATGA508150.6045442837759302No Hit
TGTAAACATCCTTGACTGGAAGCT496380.5905415558018227No Hit
TGTAAACATCCCCGACTGGAAGCT427800.5089521688464881No Hit
CATAAAGTAGAAAGCACTACT425100.5057399882576954No Hit
TTCAAGTAATTCAGGATAGGTT414520.49315299913568544No Hit
ACTGGACTTGGAGTCAGAAGGC403240.47973322245361816No Hit
TGAATACCGGGTGCTGTAGGCTTT387270.4607337691191665No Hit
CCCCCACTGCTAAATTTGACTGGCTT378970.4508592880499149No Hit
CAACGGAATCCCAAAAGCAGCTGT360280.42862386019638327No Hit
CATTGCACTTGTCTCGGTCTGA329830.3923976013338877No Hit
TGATGTAGTAGGTTGTATAGTT326810.38880471786049736No Hit
TCGTACGACTCTTAGCGG301650.35887195355900686No Hit
CAACGGAATCCCAAAAGCAGCT287610.3421686144972848No Hit
TGAATACCGGGTGCTGTAGGCTT264430.31459144929424226No Hit
TTAATGCTAATCGTGATAGGGGTT256150.304740762155278No Hit
GCCTGTCTGAGCGTCGCT238590.28384969136298177No Hit
TTCTCACTACTGCACTTGACTAG235100.2796976505278386No Hit
CTCCCACTGCTTCACTTGACTAG227940.2711794234849661No Hit
TATTGCACTTGTCCCGGCCTGTAG227650.27083441149579945No Hit
CCCATAAAGTAGAAAGCACTAC227640.2708225145306558No Hit
CCCATAAAGTAGAAAGCACT226680.26968040587686287No Hit
AACATTCAACGCTGTCGGTGAGTT226070.26895469100309854No Hit
CACTAGATTGTGAGCTCCTGGA221320.2633036325598521No Hit
CAACACCAGTCGATGGGCTGT213770.25432142387637624No Hit
CTTCTCACTACTGCACTTGACTAG210630.25058577682126176No Hit
TGATGTAGTAGATTGTATAGTT206860.24610062096209567No Hit
CACTAGATTGTGAGCTCCTGGAA205890.24494661534315904No Hit
TAGCTTATCAGACTGATGTTGACA204070.24278136768700986No Hit
TATTGCACTTGTCCCGGCCTGTAAT195030.2320265111971262No Hit
CCCCCCACTGCTAAATTTGACTGGCT181250.21563249322913972No Hit
TGAGGTAGTAGTTTGTACAGTT179420.21345534860784687No Hit
TATTGCACTTGTCCCGGCCTGTT172810.20559145464787662No Hit
CAAAGAATTCTCCTTTTGGGCT172190.20485384280896865No Hit
CTCCCACTGCTTCACTTGACTAGCCT166410.1979773969559235No Hit
TAGCAGCACGTAAATATTGGC164860.19613336735865364No Hit
TGAGAACTGAATTCCATGGGTT161520.19215978100066564No Hit
ACTTGACTTGGAGTCAGAAGGC157190.18700839509345366No Hit
ACCCCCCACTGCTAAATTTGACTG153930.18312998445661502No Hit
TTCAAGTAATCCAGGATAGGCTT152960.1819759788376784No Hit
GGAATACCGGGTGCTGTAGGCTTA149740.1781451560614145No Hit
ACTGGACTTGGAGTCAGAAGGCT147260.1751947087057827No Hit
CCCCCCACTGCTAAATTTGACTGG138410.16466589455362884No Hit
CAAAGAATTCTCCTTTTGGGCTT137990.16416622201759443No Hit
AGCAGCATTGTACAGGGCTATGT133090.15833670909719286No Hit
TGTAAACATCCTACACTCTCAGCT131600.156564061290785No Hit
TATTGCACTTGTCCCGGCCTGTAAA130060.1547319286586588No Hit
TATTGCACTTGTCCCGGCCTGTTT129610.15419656522719338No Hit
TCGTTTCCCGGCCAATGCACCA126480.15047281513722255No Hit
AGCTCGGTCTGAGGCCCCTCAGT125080.14880724001710782No Hit
AACGGAATCCCAAAAGCAGCTG123380.1467847559426828No Hit
CCCCCCACTGCTAAATTTGACTGTA119980.14273978779383273No Hit
TTATAAAGCAATGAGACTGATT112000.13324600960917873No Hit
CCCCCCACTGCTAAATTTGACTGT110830.13185406468736857No Hit
TGAGGTAGTAGTTTGTGCTGTT109640.13043832583527104No Hit
ACTGGACTTGGAGTCAGAAGGCA108760.1293913929026275No Hit
TGAGAACTGAATTCCATAGGCTGT108390.12895120519231146No Hit
GGAATACCGGGTGCTGTAGGCT106520.12672647271044393No Hit
TGCAAGTAATCCAGGATAGGCT104430.1242400069954155No Hit
CGCCCCACTGCTAAATTTGACTG103060.12261012277073179No Hit
ATGCACCTGGGCAAGGATTCTGA100110.11910051805334718No Hit
TGTAAACATCCCCGACTGGAAGC95600.113734986773549No Hit
TCCTGTCTGAGCGTCGCT95050.11308065369064679No Hit
TCCTGTACTGAGCTGCCCCGAGT94850.11284271438777324No Hit
CCCGCCACTGCTAAATTTGACTG94120.11197423593228485No Hit
CTCCCCACTGCTAAATTTGACTG93760.1115459451871125No Hit
TATGGCACTTGTCCCGGCCTGTAA92980.11061798190590572No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC92420.10995175185785983No Hit
TATGGCACTTGTCCCGGCCTGTAT86400.10278977884136647No Hit
TCCTGTACTGAGCTGCCCCGAG85970.10227820934018837No Hit
TTCAAGTAATCCAGGATAGGC84430.10044607670806216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)