FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8942513
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6539037.3122957718932025No Hit
CCCCCCACTGCTAAATTTGACTG5820316.508584331943382No Hit
GCCTGTCTGAGCGTCGCT3246373.6302658995295842No Hit
AACATTCAACGCTGTCGGTGAGT2787643.117289289934496No Hit
TTCAAGTAATCCAGGATAGGCT2494712.7897191762539233No Hit
TATTGCACTTGTCCCGGCCTGTAT2255082.5217519952165572No Hit
TAGCAGCACGTAAATATTGGCG2184182.442467794008239No Hit
AACATTCAACGCTGTCGGTGAG2180702.4385762704510467No Hit
AAGCTGCCAGTTGAAGAACTGT2119182.3697812907848164No Hit
CCCCCACTGCTAAATTTGACTG2027662.267438694246237No Hit
TATTGCACTTGTCCCGGCCTGTAA1745351.9517444369384758No Hit
TAGCTTATCAGACTGATGTTGACT1384591.5483231615095219No Hit
TATTGCACTTGTCCCGGCCTGT1218871.3630061259066664No Hit
TCCTGTCTGAGCGTCGCT1185351.325522255321295No Hit
TAGCTTATCAGACTGATGTTGAC1109421.24061323701738No Hit
TGAGGTAGTAGGTTGTATAGTT1069571.1960508192719428No Hit
TAGCTTATCAGACTGATGTTGA1063631.1894083911312179No Hit
TATTGCACTTGTCCCGGCCTGTA924041.033311329824178No Hit
TGAGGTAGTAGATTGTATAGTT852350.9531437080382215No Hit
TGTAAACATCCTTGACTGGAAGCT628060.7023305417615832No Hit
TTCAAGTAATTCAGGATAGGTT620030.693350962978751No Hit
CATAAAGTAGAAAGCACTACT570610.6380868554510348No Hit
AGCAGCATTGTACAGGGCTATGA521490.5831582240920422No Hit
CACCCGGCTGTGTGCACATGTGC490460.5484588057070758No Hit
TGTAAACATCCCCGACTGGAAGCT436180.4877599842460391No Hit
TGATGTAGTAGGTTGTATAGTT393850.44042429683915474No Hit
TTAATGCTAATCGTGATAGGGGTT382130.4273183611810237No Hit
ACTGGACTTGGAGTCAGAAGGC366300.40961640201138094No Hit
ATCCCGGACGAGCCCCCA361160.4038685769872518No Hit
CAACGGAATCCCAAAAGCAGCTGT359880.40243721200069826No Hit
CATTGCACTTGTCTCGGTCTGA345630.38650209398633245No Hit
CTCCCACTGCTTCACTTGACTAG345370.3862113479734388No Hit
CCCATAAAGTAGAAAGCACTAC337370.37726531680747905No Hit
TGATGTAGTAGATTGTATAGTT316090.3534688739060262No Hit
TCGTACGACTCTTAGCGG314930.352171699386962No Hit
CCCATAAAGTAGAAAGCACT305660.3418054857734062No Hit
CCCCCACTGCTAAATTTGACTGGCTT302250.33799223998891587No Hit
TTCTCACTACTGCACTTGACTAG291230.3256690820578063No Hit
TGAGGTAGTAGTTTGTACAGTT268720.30049718686458715No Hit
TATTGCACTTGTCCCGGCCTGTT262040.29302725084101083No Hit
CACTAGATTGTGAGCTCCTGGA252100.28191180711730585No Hit
TATTGCACTTGTCCCGGCCTGTAG239980.26835856990087686No Hit
CACTAGATTGTGAGCTCCTGGAA237610.2657083081679613No Hit
CTTCTCACTACTGCACTTGACTAG225540.25221098364631955No Hit
TATTGCACTTGTCCCGGCCTGTTT220820.24693282525840332No Hit
AGCTCGGTCTGAGGCCCCTCAGT220150.2461835951482542No Hit
TGAGAACTGAATTCCATGGGTT215920.241453381169253No Hit
ACCGGGTGCTGTAGGCTTA215730.24124091292906147No Hit
CAAAGAATTCTCCTTTTGGGCT205650.22996891365995217No Hit
GGAATACCGGGTGCTGTAGGCTTT194980.2180371445923534No Hit
CCCCCCACTGCTAAATTTGACTGGCT193340.21620320820333166No Hit
ACCGGGTGCTGTAGGCTT190390.212904359210884No Hit
CAACACCAGTCGATGGGCTGT190180.21266952589277754No Hit
CAACGGAATCCCAAAAGCAGCT184960.20683224055698884No Hit
AACATTCAACGCTGTCGGTGAGTT180750.20212439165590257No Hit
TGTAAACATCCTACACTCTCAGCT180600.20195665357154077No Hit
AACCGGGCGGAAACACCA164240.18366201983715316No Hit
CGCCTGTCTGAGCGTCGCT159080.17789182973510914No Hit
CTCCCACTGCTTCACTTGACTAGCCT157790.17644928220959813No Hit
GGAATACCGGGTGCTGTAGGCTT157330.17593488541755545No Hit
TGAGAACTGAATTCCATAGGCTGT156820.17536457593072552No Hit
TAGCTTATCAGACTGATGTTGACA154410.17266958404198016No Hit
TTCAAGTAATCCAGGATAGGCTT153450.17159606030206498No Hit
ACTTGACTTGGAGTCAGAAGGC150650.16846494939397907No Hit
TATTGCACTTGTCCCGGCCTGTAAT146950.1643274099797227No Hit
ACCGGGTGCTGTAGGCTTT144860.16199025933761574No Hit
TGAGGTAGTAGTTTGTGCTGTT143650.1606371721237643No Hit
CAAAGAATTCTCCTTTTGGGCTT142280.15910516428659371No Hit
TAGCAGCACGTAAATATTGGC142220.15903806905284903No Hit
AGCAGCATTGTACAGGGCTATGT141060.15774089453378487No Hit
CCCCCCACTGCTAAATTTGACTGG137620.1538941011324222No Hit
TTATAAAGCAATGAGACTGATT130560.1459992286284627No Hit
TGTAAACATCCCCGACTGGAAGC127890.14301349072682368No Hit
ACTGGACTTGGAGTCAGAAGGCA126640.14161567335714245No Hit
TATTGCACTTGTCCCGGCCTGA125860.14074343531846137No Hit
ACCCCCCACTGCTAAATTTGACTG122750.13726566570269452No Hit
AACGGAATCCCAAAAGCAGCTG119260.1333629596065446No Hit
ACTGGACTTGGAGTCAGAAGGCT111330.12449520621328701No Hit
TTCAAGTAATCCAGGATAGGC110900.1240143570381167No Hit
TGAGGTAGTAGGTTGTATAGT106470.11906049227996648No Hit
CCCCCCACTGCTAAATTTGACTGT104480.11683516702743402No Hit
TATTGCACTTGTCCCGGCCTGTAAA103030.1152136988786038No Hit
TTCACAGTGGCTAAGTTCCGC99140.11086369122415589No Hit
TGCAAGTAATCCAGGATAGGCT98880.11057294521126221No Hit
TGATGTAGTAGTTTGTACAGTT98830.11051703251647495No Hit
TCGTTTCCCGGGCGGCGCACCA91740.10258861239564314No Hit
TTCACAGTGGCTAAGTTCCG91610.10244323938919632No Hit
CCCATAAAGTAGAAAGCACTA90880.10162691404530248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)