FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6495065
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5417118.340347633164564No Hit
CAACGGAATCCCAAAAGCAGCTG4551907.0082439513692325No Hit
AACATTCAACGCTGTCGGTGAGT3364355.179855782813567No Hit
TTCAAGTAATCCAGGATAGGCT2050153.1564734148157103No Hit
CCCCCACTGCTAAATTTGACTG1979273.0473444068689073No Hit
AACATTCAACGCTGTCGGTGAG1938292.984250350073479No Hit
AAGCTGCCAGTTGAAGAACTGT1585392.4409147560494002No Hit
TAGCAGCACGTAAATATTGGCG1488992.2924943784242346No Hit
TATTGCACTTGTCCCGGCCTGTAT1040301.6016775813636968No Hit
TATTGCACTTGTCCCGGCCTGTAA1026251.580045773213971No Hit
TAGCTTATCAGACTGATGTTGACT954391.4694079274033438No Hit
TAGCTTATCAGACTGATGTTGA925651.4251589476009863No Hit
TAGCTTATCAGACTGATGTTGAC842481.2971078811374481No Hit
TGAGGTAGTAGGTTGTATAGTT799591.2310731301380358No Hit
TATTGCACTTGTCCCGGCCTGT681091.048626919053158No Hit
TGAGGTAGTAGATTGTATAGTT606370.9335857300889214No Hit
TATTGCACTTGTCCCGGCCTGTA516340.7949727985786131No Hit
CATAAAGTAGAAAGCACTACT486990.7497846441875485No Hit
TGTAAACATCCTTGACTGGAAGCT439010.6759131740790892No Hit
CTCCCACTGCTTCACTTGACTAG385070.5928655063498209No Hit
TTCTCACTACTGCACTTGACTAG374900.5772074644364606No Hit
AGCAGCATTGTACAGGGCTATGA372550.5735893328242289No Hit
CACCCGGCTGTGTGCACATGTGC349970.5388244767373384No Hit
TTCAAGTAATTCAGGATAGGTT341690.5260763364184962No Hit
TGTAAACATCCCCGACTGGAAGCT316280.4869543260921946No Hit
ACTGGACTTGGAGTCAGAAGGC309410.4763770647406916No Hit
CGCCTGTCTGAGCGTCGCT308580.4750991714478608No Hit
CTTCTCACTACTGCACTTGACTAG296090.4558691868364674No Hit
CCCATAAAGTAGAAAGCACT255620.39356034158241676No Hit
CATTGCACTTGTCTCGGTCTGA239380.3685567426961855No Hit
CCCATAAAGTAGAAAGCACTAC237100.36504638521708405No Hit
CAACGGAATCCCAAAAGCAGCTGT233040.35879548549552626No Hit
AACATTCAACGCTGTCGGTGAGTT224750.3460319488719512No Hit
TGAGGTAGTAGTTTGTACAGTT221870.34159781310887577No Hit
TGATGTAGTAGGTTGTATAGTT207270.3191192081988402No Hit
CCCCCACTGCTAAATTTGACTGGCTT204310.31456190199790146No Hit
ACCGGGTGCTGTAGGCTT192680.29665599959353756No Hit
CAACACCAGTCGATGGGCTGT174470.2686193286749248No Hit
ACCGGGTGCTGTAGGCTTA155680.23968967208180364No Hit
TGATGTAGTAGATTGTATAGTT154410.2377343413807252No Hit
CTCCCACTGCTTCACTTGACTAGCCT150220.23128328969763967No Hit
CCCCCCACTGCTAAATTTGACTGGCT146960.2262640943547139No Hit
CCCCCCACTGCTAAATTTGACTGG145880.2246012934435606No Hit
GGAATACCGGGTGCTGTAGGCTTT133960.20624889820194253No Hit
TATTGCACTTGTCCCGGCCTGTT131840.2029848815985675No Hit
TCGTACGACTCTTAGCGG131840.2029848815985675No Hit
ACCGGGTGCTGTAGGCTTT127950.19699571905746902No Hit
TTCAAGTAATCCAGGATAGGCTT126500.19476325487119836No Hit
CACTAGATTGTGAGCTCCTGGA124920.1923306387234No Hit
TGAGGTAGTAGTTTGTGCTGTT124730.19203810893347487No Hit
CTACGCCTGTCTGAGCGTCGCT124440.19159161609622075No Hit
TGTAAACATCCTACACTCTCAGCT121260.1866955911911582No Hit
AGCTCGGTCTGAGGCCCCTCAGT119540.18404742677709923No Hit
CAACGGAATCCCAAAAGCAGCT117800.18136846975357446No Hit
TATTGCACTTGTCCCGGCCTGTAG116980.18010597276547655No Hit
GGAATACCGGGTGCTGTAGGCTT113490.1747326624136941No Hit
CAAAGAATTCTCCTTTTGGGCT113480.17471726610896118No Hit
AACATTCAACGCTGTCGGTGA111150.17112992710619526No Hit
ATCCCGGACGAGCCCCCA110010.1693747483666445No Hit
TAGCTTATCAGACTGATGTTGACA106200.16350875626340922No Hit
CACTAGATTGTGAGCTCCTGGAA103500.15935175398552592No Hit
ACTGGACTTGGAGTCAGAAGGCT103000.15858193874888088No Hit
ACCCCCCACTGCTAAATTTGACTG100920.15537950736443745No Hit
TTATAAAGCAATGAGACTGATT100490.15471746626092273No Hit
TTCACAGTGGCTAAGTTCCG98970.15237722794152175No Hit
TTCACAGTGGCTAAGTTCTG96990.14932875960440736No Hit
AACGGAATCCCAAAAGCAGCTG96880.14915940025234542No Hit
TTCAAGTAATCCAGGATAGGC95410.14689614345660898No Hit
GCCTGTCTGAGCGTCGCT94020.14475605709873574No Hit
TCCCACTGCTTCACTTGACTAG88020.1355182742589951No Hit
TGTAAACATCCCCGACTGGAAGC87660.13496400728861066No Hit
AGCAGCATTGTACAGGGCTATGT87490.13470227010815133No Hit
ACTGGACTTGGAGTCAGAAGG85260.1312688941527144No Hit
TGAGGTAGTAGGTTGTATAGT84750.13048368261133644No Hit
CAAAGAATTCTCCTTTTGGGCTT83140.1280048775493394No Hit
ACTTGACTTGGAGTCAGAAGGC81420.1253567131352804No Hit
AACATTCAACGCTGTCGGTGAGA80880.12452531267970375No Hit
TATTGCACTTGTCCCGGCCTGTTT80680.12421738658504572No Hit
TGTCAGTTTGTCAAATACCCCAA80030.12321662677740716No Hit
GACCCGCGGGCGCTCTCCAGTCCT79950.12309345633954394No Hit
CAAAGTGCTGTTCGTGCAGGT79310.12210809283663827No Hit
AGTTCTACAGTCCGACGATC78890.12146144803785644No Hit
TGAGGTAGTAGGTTGTGTGGTT78650.12109193672426681No Hit
TAGTGGGTTATCAGAACT78330.12059925497281397No Hit
TAGCAGCACGTAAATATTGGC77040.11861313166226975No Hit
ACTGGACTTGGAGTCAGAAGGCA76720.1181204499108169No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC75730.1165962157422597No Hit
TGTCAGTTTGTCAAATACCCCA75090.11561085223935404No Hit
AGCAGCATTGTACAGGGCTATG74030.11397884393766651No Hit
TACGCCTGTCTGAGCGTCGCT72150.11108433864788113No Hit
TTCACAGTGGCTAAGTTCTGC70080.10789730356817061No Hit
ACCACAGGGTAGAACCACGGAC68390.10529532806831032No Hit
TATTGCACTTGTCCCGGCCTGTAAT68250.10507977980204972No Hit
AACATTCATTGCTGTCGGTGGGT68180.1049720056689194No Hit
CCCATAAAGTAGAAAGCACTA67900.10454090913639817No Hit
ACGCCTGTCTGAGCGTCGCT66490.10237003016905913No Hit
CCCCCACTGCTAAATTTGACTGGCT65570.10095357013363224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)