FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454534
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGG89920116.485386285977867No Hit
CAACGGAATCCCAAAAGCAGCTG60789711.144801737416982No Hit
AACATTCAACGCTGTCGGTGAGT1845623.3836437723185884No Hit
AACATTCAACGCTGTCGGTGAG1771263.247316819365321No Hit
CCCCCCACTGCTAAATTTGACTG1558722.8576593344179355No Hit
TAGCAGCACGTAAATATTGGCG919071.6849652050935975No Hit
TTCAAGTAATCCAGGATAGGCT848481.5555499333215266No Hit
TATTGCACTTGTCCCGGCCTGT729831.3380244765180673No Hit
AAGCTGCCAGTTGAAGAACTGT679851.2463942840946631No Hit
TGAGGTAGTAGGTTGTATAGTT632641.1598424356691148No Hit
TAGCTTATCAGACTGATGTTGAC495740.9088585752696746No Hit
CCCCCACTGCTAAATTTGACTG484880.8889485334585868No Hit
TATTGCACTTGTCCCGGCCTGTA337400.6185679656593945No Hit
TAGCTTATCAGACTGATGTTGA280110.5135360784257647No Hit
CTCGTACGACTCTTAGCGG252020.46203763694570427No Hit
CACCCGGCTGTGTGCACATGTGC248280.45518095588000734No Hit
TTCACCACCTTCTCCACCCAGC247800.4543009540320035No Hit
TGAGGTAGTAGGTTGTGTGGTT222470.40786252317796534No Hit
TATTGCACTTGTCCCGGCCTGTAT218440.4004741743290994No Hit
ACTGGACTTGGAGTCAGAAGGC213530.39147248875889307No Hit
ATCCCACCGCTGCCACCA203610.3732857839001462No Hit
AACATTCAACGCTGTCGGTGAGTT200760.3680607729276232No Hit
AGCAGCATTGTACAGGGCTATGA200540.367657438747288No Hit
TATTGCACTTGTCCCGGCCTGTAA198430.3637890972904376No Hit
CCCCCACTGCTAAATTTGACTGGCTT184970.33911237880266215No Hit
TCGGACGACTCTTAGCGG173780.3185973357210717No Hit
TAGCTTATCAGACTGATGTTGACT168030.30805564691685855No Hit
CATTGCACTTGTCTCGGTCTGA158570.29071227716244874No Hit
AGCTCGGTCTGAGGCCCCTCAGT143010.2621855505896562No Hit
CATAAAGTAGAAAGCACTACT142680.2615805493191536No Hit
ACCGGGTGCTGTAGGCTT137260.2516438617854431No Hit
TGAGGTAGTAGATTGTATAGTT135780.24893052275409777No Hit
TGAGGTAGTAGTTTGTGCTGTT133110.2440355124745762No Hit
CTATACGACCTGCTGCCTTTCT126250.23145881939685406No Hit
TCCCCGGCACCTCCACCA124880.22894714745567632No Hit
GTCCGGCCGTGTCGGCGG123600.22660047586099932No Hit
AACGGAATCCCAAAAGCAGCTG122950.2254088066918274No Hit
CGCCTGTCTGAGCGTCGCT120860.22157713197864382No Hit
CAACGGAATCCCAAAAGCAGCTGT117030.21455545056644618No Hit
TTCAAGTAATTCAGGATAGGTT116570.2137121154621091No Hit
TGAGGGGCAGAGAGCGAGACTTT116000.2126671132676045No Hit
TATTGCACTTGTCCCGGCCTGTT114100.20918377261925583No Hit
ATCCCGGACGAGCCCCCA109450.20065875471671824No Hit
TGTAAACATCCCCGACTGGAAGCT106860.19591041141186397No Hit
CTACGCCTGTCTGAGCGTCGCT105300.19305040540585136No Hit
ACCGGGTGCTGTAGGCTTT104910.1923354039043482No Hit
CTCCCACTGCTTCACTTGACTAG103220.18923706406450122No Hit
CGCGACCTCAGATCAGACGTGG100810.18481872145264838No Hit
CAACGGAATCCCAAAAGCAGCT93570.17154536024525652No Hit
AATGCACCCGGGCAAGGATTCT91450.16765868541657272No Hit
GCGTACGACTCTTAGCGG91110.16703535077423662No Hit
TGATGTAGTAGGTTGTATAGTT86640.15884033356470048No Hit
TTCACAGTGGCTAAGTTCCGC85190.15618199464885543No Hit
CCCCCCACTGCTAAATTTGACTGGCT84050.1540919902598462No Hit
TCCTGTACTGAGCTGCCCCGAGT82650.15152531820316822No Hit
TGTAAACATCCTTGACTGGAAGCT81860.1500769818283285No Hit
TCCACCACGTTCCCGTGG77550.142175298568127No Hit
ACCCCCCACTGCTAAATTTGACTG76660.14054362847495314No Hit
TAGCAGCACGTAAATATTGGC76440.14014029429461802No Hit
CCCCCCACTGCTAAATTTGACTGG76410.14008529417911778No Hit
TCCTGTACTGAGCTGCCCCGAGA75860.13907695872828No Hit
GTCAGGATGGCCGAGCGGTCTAAGG72910.13366861403742283No Hit
AAAAGCTGGGTTGAGAGGGCGA71400.1309002748905773No Hit
GGAATACCGGGTGCTGTAGGCTTT71150.1304419405947419No Hit
TAGCTTATCAGACTGATGTTGACA69830.12802193551273125No Hit
AACATTCAACGCTGTCGGTGAGA69190.12684859971539272No Hit
GGGAATACCGGGTGCTGTAGGCTT67660.12404359382488038No Hit
GTGGGTGGTGGTGCATGGCCG65710.12046858631736461No Hit
CAACACCAGTCGATGGGCTGT65510.12010191888069631No Hit
GGAATACCGGGTGCTGTAGGCTT65010.11918525028902562No Hit
GGGAATACCGGGTGCTGTAGGCTTT62640.11484024116450643No Hit
TGTAAACATCCTACACTCTCAGCT62490.11456524058700522No Hit
CACTAGATTGTGAGCTCCTGGA61570.11287857037833113No Hit
TGAGGTAGTAGGTTGTGTGGTTT60740.11135690051615775No Hit
TCCTGTACTGAGCTGCCCCGAG60450.11082523273298875No Hit
CTCCCACTGCTTCACTTGACTAGCCT60390.11071523250198824No Hit
CGCGACCTCAGATCAGACGTGGC58070.10646189023663616No Hit
AACATTCATTGCTGTCGGTGGGTT57110.1047018865406284No Hit
CTTCTCACTACTGCACTTGACTAG55580.10189688065011603No Hit
TTCACAGTGGCTAAGTTCTGC55170.10114521240494605No Hit
CCCATAAAGTAGAAAGCACT54830.10052187776260996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)