FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8756970
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG87764310.022222298352055No Hit
CCCCCCACTGCTAAATTTGACTG4791815.47199545048116No Hit
GCCTGTCTGAGCGTCGCT3200893.6552483336131107No Hit
AACATTCAACGCTGTCGGTGAGT2645173.02064526885441No Hit
TTCAAGTAATCCAGGATAGGCT2504052.859493637639503No Hit
TCGTACGACTCTTAGCGG2087272.3835527585454788No Hit
TATTGCACTTGTCCCGGCCTGTAA2067602.361090651218401No Hit
AACATTCAACGCTGTCGGTGAG2002892.2871952284865658No Hit
TAGCAGCACGTAAATATTGGCG1787672.0414252875138317No Hit
TATTGCACTTGTCCCGGCCTGTAT1737761.9844306877835598No Hit
AAGCTGCCAGTTGAAGAACTGT1708431.9509373676054618No Hit
CCCCCACTGCTAAATTTGACTG1439061.643330969502008No Hit
TGAGGTAGTAGGTTGTATAGTT1397301.5956432418975972No Hit
TATTGCACTTGTCCCGGCCTGT1210601.382441643627876No Hit
TATTGCACTTGTCCCGGCCTGTA793040.9056100454837689No Hit
CAACGGAATCCCAAAAGCAGCTGT554240.6329129824585444No Hit
ACTGGACTTGGAGTCAGAAGGC535830.6118897289815999No Hit
TGAGGTAGTAGATTGTATAGTT517210.5906266665296329No Hit
TAGCTTATCAGACTGATGTTGACT489420.5588919455016975No Hit
TAGCTTATCAGACTGATGTTGAC475040.5424707404501785No Hit
TTAATGCTAATCGTGATAGGGGTT436600.4985742785461181No Hit
AGCAGCATTGTACAGGGCTATGA409320.46742195074323656No Hit
CTCCCACTGCTTCACTTGACTAG380930.4350020612152377No Hit
CGCCTGTCTGAGCGTCGCT377430.43100524496486803No Hit
TAGCTTATCAGACTGATGTTGA368210.42047648901389406No Hit
AGCTCGGTCTGAGGCCCCTCAGT366720.41877498723873663No Hit
TTCAAGTAATTCAGGATAGGTT339470.38765691786085826No Hit
CCCCCACTGCTAAATTTGACTGGCTT337910.3858754797606935No Hit
TGTAAACATCCTTGACTGGAAGCT328130.37470723320966043No Hit
TGTAAACATCCCCGACTGGAAGCT326340.3726631471844713No Hit
CACCCGGCTGTGTGCACATGTGC312660.3570413053830263No Hit
AACCGGGCGGAAACACCA309230.353124425457664No Hit
GGAATACCGGGTGCTGTAGGCTTT302880.34587305883199326No Hit
CATAAAGTAGAAAGCACTACT247890.28307736580118464No Hit
CTACGCCTGTCTGAGCGTCGCT243230.27775589045069243No Hit
TGAGGTAGTAGTTTGTACAGTT241840.27616858342554557No Hit
CATTGCACTTGTCTCGGTCTGA234900.2682434677748125No Hit
GGAATACCGGGTGCTGTAGGCTT231260.26408677887442805No Hit
TATTGCACTTGTCCCGGCCTGTAG226820.259016531973959No Hit
CCCCCCACTGCTAAATTTGACTGGCT221660.253124082873414No Hit
TTCTCACTACTGCACTTGACTAG216600.2473458285228795No Hit
TGAGGTAGTAGGTTGTGTGGTT215630.24623813944777703No Hit
TATTGCACTTGTCCCGGCCTGTT209540.23928367917213375No Hit
CCCATAAAGTAGAAAGCACTAC208150.2376963721469869No Hit
AACGGAATCCCAAAAGCAGCTG197690.22575160129588204No Hit
CAACGGAATCCCAAAAGCAGCT196560.22446120062076264No Hit
TGAGGTAGTAGTTTGTGCTGTT178980.2043857635689057No Hit
AACATTCAACGCTGTCGGTGAGTT178170.20346078609382012No Hit
TCGTTTCCCGGCCAATGCACCA177010.20213612699369757No Hit
TATTGCACTTGTCCCGGCCTGTAAT176500.20155373376864374No Hit
TCGTTTCCCGGGCGGCGCACCA174570.19934977509343987No Hit
CCCCCCACTGCTAAATTTGACTGG161300.18419613176703814No Hit
CTTCTCACTACTGCACTTGACTAG160610.18340818799196526No Hit
ATCCCGGACGAGCCCCCA157920.1803363492166811No Hit
TGATGTAGTAGGTTGTATAGTT153450.17523184389120894No Hit
ACCCCCCACTGCTAAATTTGACTG152360.17398712111609382No Hit
TACGCCTGTCTGAGCGTCGCT150490.1718516792908963No Hit
CACTAGATTGTGAGCTCCTGGA148680.16978475431570508No Hit
TATTGCACTTGTCCCGGCCTGTAAA147510.1684486757405815No Hit
CCCATAAAGTAGAAAGCACT143220.16354972096512835No Hit
TAGCAGCACGTAAATATTGGC138950.15867360513967732No Hit
ACCGGGTGCTGTAGGCTT138880.1585936688146699No Hit
ACTGGACTTGGAGTCAGAAGGCT138590.1582625040396393No Hit
GTCAGGATGGCCGAGCGGTCTAAGG133980.15299812606415233No Hit
TCCTGTCTGAGCGTCGCT133500.15244999126410164No Hit
TGAGAACTGAATTCCATGGGTT133050.1519361148890541No Hit
ACTGGACTTGGAGTCAGAAGGCA132790.15163920853902663No Hit
ACCGGGTGCTGTAGGCTTA129090.1474140027886358No Hit
TATTGCACTTGTCCCGGCCTGTTT128470.1467059953385703No Hit
CACTAGATTGTGAGCTCCTGGAA127470.1455640478384647No Hit
ACCGGGTGCTGTAGGCTTT121170.1383697785877992No Hit
CAAAGAATTCTCCTTTTGGGCT120750.13789016063775483No Hit
GACCCGCGGGCGCTCTCCAGTCCT116390.1329112695372943No Hit
TGTAAACATCCTACACTCTCAGCT114920.13123260671213902No Hit
CTCCCACTGCTTCACTTGACTAGCCT106950.12213128513629715No Hit
CAACACCAGTCGATGGGCTGT103530.1182258246859359No Hit
AGAGGTAGTAGGTTGCATAGTT103390.11806595203592109No Hit
ACGCCTGTCTGAGCGTCGCT103100.11773478726089047No Hit
TTCACCACCTTCTCCACCCAGC102890.11749497828586829No Hit
ATGCACCTGGGCAAGGATTCTGA101900.11636445026076371No Hit
GGGAATACCGGGTGCTGTAGGCTT101850.11630735288575844No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC101710.11614748023574363No Hit
TATTGCACTTGTCCCGGCCTGA101470.1158734128357183No Hit
TGAGGTAGTAGGTTGTATAGT101440.11583915441071511No Hit
TCCCACTGCTTCACTTGACTAG100880.11519966381065598No Hit
TTCAAGTAATCCAGGATAGGCTT100290.11452591478559365No Hit
CGCGACCTCAGATCAGACGTGGCG98780.11280157406043416No Hit
AATGCACCCGGGCAAGGATTCT96200.10985534951016161No Hit
GGGAATACCGGGTGCTGTAGGCTTT95600.10917018101009825No Hit
TTCAAGTAATCCAGGATAGGC92570.10571008008477818No Hit
CAAAGAATTCTCCTTTTGGGCTT91230.10417987043463664No Hit
TGAGAACTGAATTCCATAGGCTGT90060.10284379185951303No Hit
CGCGACCTCAGATCAGACGTGG88590.10116512903435776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)