FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6118115
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4544537.427990483997113No Hit
CCCCCCACTGCTAAATTTGACTG3489485.703521427760021No Hit
TTCAAGTAATCCAGGATAGGCT2071303.3855198864356097No Hit
AACATTCAACGCTGTCGGTGAGT1708182.7920037462519094No Hit
TAGCAGCACGTAAATATTGGCG1600072.615298993235662No Hit
AAGCTGCCAGTTGAAGAACTGT1356192.216679483795254No Hit
AACATTCAACGCTGTCGGTGAG1117351.8262978057784138No Hit
TATTGCACTTGTCCCGGCCTGTAA1018821.6652514704283916No Hit
CCCCCACTGCTAAATTTGACTG983541.607586650463419No Hit
TATTGCACTTGTCCCGGCCTGTAT863861.4119708439609258No Hit
TAGCTTATCAGACTGATGTTGAC709051.158935391047733No Hit
TGAGGTAGTAGGTTGTATAGTT703131.1492592081057647No Hit
TAGCTTATCAGACTGATGTTGACT654391.0695941478707085No Hit
GGAATACCGGGTGCTGTAGGCTTT619551.012648503664936No Hit
TTCAAGTAATTCAGGATAGGTT578560.945650743733977No Hit
TATTGCACTTGTCCCGGCCTGT559040.9137454918712709No Hit
TGTAAACATCCTTGACTGGAAGCT512360.8374474817815619No Hit
TTAATGCTAATCGTGATAGGGGTT502430.8212169924886995No Hit
TGAGGTAGTAGATTGTATAGTT448790.7335429294807306No Hit
GCCTGTCTGAGCGTCGCT405570.6629002560429152No Hit
TAGCTTATCAGACTGATGTTGA397070.649007087967454No Hit
TATTGCACTTGTCCCGGCCTGTA386540.6317959044575004No Hit
GGAATACCGGGTGCTGTAGGCTT370570.6056930933792516No Hit
TCGTACGACTCTTAGCGG339090.5542393367891908No Hit
CAACGGAATCCCAAAAGCAGCTGT321160.5249329246017769No Hit
CAACGGAATCCCAAAAGCAGCT317040.5181988243110828No Hit
AGCAGCATTGTACAGGGCTATGA316200.5168258524071548No Hit
TGTAAACATCCCCGACTGGAAGCT308980.505024831994822No Hit
CCCCCACTGCTAAATTTGACTGGCTT285720.467006586178913No Hit
AACCGGGCGGAAACACCA271640.443992961884502No Hit
ACTGGACTTGGAGTCAGAAGGC256540.4193121574210357No Hit
CATAAAGTAGAAAGCACTACT234610.38346778378634594No Hit
CCCATAAAGTAGAAAGCACTAC218660.35739766251533356No Hit
TCGTTTCCCGGCCAATGCACCA218460.35707076444296976No Hit
TGAATACCGGGTGCTGTAGGCTTT208580.34092199966819847No Hit
CTACGCCTGTCTGAGCGTCGCT207540.33922212969190674No Hit
CTCCCACTGCTTCACTTGACTAG199320.3257866189177549No Hit
TTCTCACTACTGCACTTGACTAG194150.3173363037471509No Hit
GGGAATACCGGGTGCTGTAGGCTTT185340.3029364436595258No Hit
CTCCCACTGCTTCACTTGACTAGCCT183200.29943863428523326No Hit
CCCCCCACTGCTAAATTTGACTGGCT176510.28850389376466445No Hit
TGATGTAGTAGGTTGTATAGTT173700.28391097584795316No Hit
CGCCTGTCTGAGCGTCGCT172490.2819332425101522No Hit
CATTGCACTTGTCTCGGTCTGA168110.2747741747253852No Hit
TGAGGTAGTAGTTTGTACAGTT161800.26446054054230755No Hit
CAAAGAATTCTCCTTTTGGGCT156630.25601022537170354No Hit
TGTAAACATCCTACACTCTCAGCT154670.2528066242625384No Hit
GGGAATACCGGGTGCTGTAGGCTT146920.24013932395844143No Hit
CTTCTCACTACTGCACTTGACTAG143020.23376481154734752No Hit
AGCTCGGTCTGAGGCCCCTCAGT142900.23356867270392925No Hit
GAATACCGGGTGCTGTAGGCTTT137810.22524911676227075No Hit
TCCTGTCTGAGCGTCGCT127750.208806143722372No Hit
CACCCGGCTGTGTGCACATGTGC126950.20749855143291684No Hit
TAGCAGCACGTAAATATTGGC124470.2034450153356058No Hit
CCCATAAAGTAGAAAGCACT124250.20308542745600563No Hit
TGAATACCGGGTGCTGTAGGCTT122660.2004865877807135No Hit
GGAATACCGGGTGCTGTAGGCTTA120700.19728298667154834No Hit
TGAGAACTGAATTCCATGGGTT119320.19502738997223817No Hit
TGTCAGTTTGTCAAATACCCCAA118090.19301696682720088No Hit
CAAAGAATTCTCCTTTTGGGCTT116620.190614265995327No Hit
AACATTCAACGCTGTCGGTGAGTT116390.19023833321210865No Hit
TATTGCACTTGTCCCGGCCTGTAG113620.1857107949098701No Hit
TGATGTAGTAGATTGTATAGTT110690.18092173814974055No Hit
ACCGGGTGCTGTAGGCTT108430.1772277899320297No Hit
TGAGGTAGTAGTTTGTGCTGTT106360.17384439488306447No Hit
TAGCTTATCAGACTGATGTTGACA103990.16997065272555356No Hit
CCCCCCACTGCTAAATTTGACTGG103980.16995430782193535No Hit
TTCAAGTAATCCAGGATAGGCTT103320.16887554418313483No Hit
ACCGGGTGCTGTAGGCTTT101020.16511621635095125No Hit
CAACGGAATCCCAAAAGCAGCTGA100170.16372689954340514No Hit
ACCGGGTGCTGTAGGCTTA99370.16241930725394996No Hit
GAATACCGGGTGCTGTAGGCTT98250.1605886780487127No Hit
CACTAGATTGTGAGCTCCTGGA98170.1604579188197672No Hit
TGAGAACTGAATTCCATAGGCTGT98160.16044157391614902No Hit
ACCCCCCACTGCTAAATTTGACTG96720.1580879077951297No Hit
TACGCCTGTCTGAGCGTCGCT95750.15650245214416533No Hit
TATTGCACTTGTCCCGGCCTGTT95700.15642072762607437No Hit
CACTAGATTGTGAGCTCCTGGAA95000.15527658437280112No Hit
ATCCCGGACGAGCCCCCA93690.15313540199881828No Hit
TATTGCACTTGTCCCGGCCTGTAAT92640.15141918711890837No Hit
TCGTTTCCCGGGCGGCGCACCA91230.14911455570874363No Hit
TGTCAGTTTGTCAAATACCCCA89350.146041713828524No Hit
AACGGAATCCCAAAAGCAGCTG88700.14497929509334165No Hit
ATACCGGGTGCTGTAGGCTTA87400.14285445762297702No Hit
TTCAAGTAATCCAGGATAGGC84110.13747698433259264No Hit
AATGCACCCGGGCAAGGATTCT82560.13494352427177325No Hit
ATGCACCTGGGCAAGGATTCTGA80560.13167454354813532No Hit
TATTGCACTTGTCCCGGCCTGTAAA80440.13147840470471706No Hit
TTCACAGTGGCTAAGTTCTGC79640.13017081241526188No Hit
TGAGGTAGTAGGTTGTGTGGTT78890.12894494464389766No Hit
TGTAAACATCCCCGACTGGAAGC78010.127506593125497No Hit
ATACCGGGTGCTGTAGGCTTT72080.11781406527991056No Hit
CACCCGTAGAACCGACCTTGCG70920.11591805646020056No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT69450.1135153556283267No Hit
CAACACCAGTCGATGGGCTGT69420.11346632091747214No Hit
TATTGCACTTGTCCCGGCCTGTTT66250.10828498647050602No Hit
TTAATGCTAATCGTGATAGGGGTTT64400.10526117930114096No Hit
CCCCCACTGCTAAATTTGACTGGCT63870.10439489940937692No Hit
TGGAATACCGGGTGCTGTAGGCTTT63500.1037901379755039No Hit
AGCAGCATTGTACAGGGCTATGT62880.10277675395117614No Hit
ACGCCTGTCTGAGCGTCGCT62270.10177971483046658No Hit
ACTTGACTTGGAGTCAGAAGGC61750.10092977984232072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)