FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10196917
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6027055.910659074698755No Hit
CCCCCCACTGCTAAATTTGACTG5977115.861683487273654No Hit
TTCAAGTAATCCAGGATAGGCT3139723.079087532045225No Hit
AAGCTGCCAGTTGAAGAACTGT2956942.8998372743447844No Hit
AACATTCAACGCTGTCGGTGAGT2906862.8507243905192126No Hit
TAGCAGCACGTAAATATTGGCG2554112.504786495761415No Hit
GCCTGTCTGAGCGTCGCT2145122.103694675557328No Hit
TAGCTTATCAGACTGATGTTGAC1786211.7517157391788125No Hit
CCCCCACTGCTAAATTTGACTG1569131.5388278633630146No Hit
AACCGGGCGGAAACACCA1565841.5356013979519496No Hit
TATTGCACTTGTCCCGGCCTGTAA1549371.5194494571251291No Hit
AACATTCAACGCTGTCGGTGAG1493621.4647760690804879No Hit
TATTGCACTTGTCCCGGCCTGTAT1421131.3936859542938322No Hit
TAGCTTATCAGACTGATGTTGACT1370951.344475001610781No Hit
TGAGGTAGTAGGTTGTATAGTT1251361.2271944549514329No Hit
TATTGCACTTGTCCCGGCCTGT1051461.0311548088505575No Hit
TGTAAACATCCTTGACTGGAAGCT995930.9766971722923703No Hit
TAGCTTATCAGACTGATGTTGA902110.8846889702054063No Hit
TTAATGCTAATCGTGATAGGGGTT888430.8712731505022547No Hit
AGCAGCATTGTACAGGGCTATGA887810.8706651235858839No Hit
TGAGGTAGTAGATTGTATAGTT752790.7382525522174986No Hit
TATTGCACTTGTCCCGGCCTGTA743020.728671224841783No Hit
TTCAAGTAATTCAGGATAGGTT737230.7229930379937387No Hit
CGCCTGTCTGAGCGTCGCT618920.606967772710124No Hit
ACTGGACTTGGAGTCAGAAGGC605180.5934931116924851No Hit
TGTAAACATCCCCGACTGGAAGCT547590.5370152566702269No Hit
CATAAAGTAGAAAGCACTACT508850.4990233812827936No Hit
GGAATACCGGGTGCTGTAGGCTTT471230.4621298770991271No Hit
TCGTACGACTCTTAGCGG464760.45578482202022436No Hit
CAACGGAATCCCAAAAGCAGCTGT413980.4059854561922981No Hit
CCCATAAAGTAGAAAGCACTAC370970.363806040590504No Hit
CCCCCACTGCTAAATTTGACTGGCTT366060.3589908596882764No Hit
TGTAAACATCCTACACTCTCAGCT357620.35071384811703377No Hit
CTACGCCTGTCTGAGCGTCGCT327770.3214402941594994No Hit
GGAATACCGGGTGCTGTAGGCTT325980.3196848616106221No Hit
TTCTCACTACTGCACTTGACTAG314360.3082892603715417No Hit
AACTTAACTTGACCGCTCTGACCA313750.3076910403409187No Hit
TACGCCTGTCTGAGCGTCGCT307880.30193439840689107No Hit
AACATTCAACGCTGTCGGTGAGTT302230.2963935079593175No Hit
CCCCCCACTGCTAAATTTGACTGGCT294780.2890873780771188No Hit
CTCCCACTGCTTCACTTGACTAG292670.28701812518430814No Hit
TGAGGTAGTAGTTTGTACAGTT277540.2721803070477086No Hit
CATTGCACTTGTCTCGGTCTGA273830.26854195243523116No Hit
AGCTCGGTCTGAGGCCCCTCAGT270840.26560969359660375No Hit
CTTCTCACTACTGCACTTGACTAG244280.23956260505013427No Hit
CAAAGAATTCTCCTTTTGGGCT233350.2288436789276602No Hit
ACCGGGTGCTGTAGGCTT231450.22698037063555582No Hit
CCCATAAAGTAGAAAGCACT211800.2077098401408975No Hit
ACCGGGTGCTGTAGGCTTA206700.20270832840945946No Hit
TAGCTTATCAGACTGATGTTGACA205940.2019630050926177No Hit
TGAGGTAGTAGTTTGTGCTGTT204630.20067830305964046No Hit
TTCAAGTAATCCAGGATAGGCTT198510.1946764889819148No Hit
ACCGGGTGCTGTAGGCTTT191630.18792935158734744No Hit
ACGCCTGTCTGAGCGTCGCT191190.18749784861443905No Hit
GCGTTCGCGCTTTCCCCTG190500.18682117349783273No Hit
CAACGGAATCCCAAAAGCAGCT187700.18407524548841578No Hit
CCCCCCACTGCTAAATTTGACTGG187490.1838693008877095No Hit
TATTGCACTTGTCCCGGCCTGTT186660.18305532937063232No Hit
GGGCTACGCCTGTCTGAGCGTCGCT183190.1796523400161049No Hit
TATTGCACTTGTCCCGGCCTGTAG179530.17606301983236697No Hit
AGCAGCATTGTACAGGGCTATGT178470.1750234899430877No Hit
TTCACAGTGGCTAAGTTCCG177050.17363091216688337No Hit
TCGTTTCCCGGCCAATGCACCA164300.16112713283828828No Hit
GAATACCGGGTGCTGTAGGCTTT161130.15801835005619835No Hit
TTATAAAGCAATGAGACTGATT159670.15658654473700237No Hit
CAAAGAATTCTCCTTTTGGGCTT158360.15530184270402514No Hit
CTCCCACTGCTTCACTTGACTAGCCT157840.15479188464513344No Hit
TCCTGTCTGAGCGTCGCT156590.1535660239266437No Hit
CACTAGATTGTGAGCTCCTGGA154950.1519576946639852No Hit
CACTAGATTGTGAGCTCCTGGAA144790.1419938987441008No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT140440.13772790344375657No Hit
TGATGTAGTAGGTTGTATAGTT138700.1360215053236189No Hit
TGAGGTAGTAGGTTGTGTGGTT138240.1355703885792147No Hit
CAAAGTGCTGTTCGTGCAGGTAG137950.13528598889252505No Hit
TAGCAGCACGTAAATATTGGC137460.1348054514908771No Hit
CACCCGGCTGTGTGCACATGTGC135040.132432185139881No Hit
TTCAAGTAATCCAGGATAGGC134140.1315495654225684No Hit
CAACACCAGTCGATGGGCTGT133510.1309317316204496No Hit
GGGAATACCGGGTGCTGTAGGCTTT132900.13033351158982662No Hit
TCGTTTCCCGGGCGGCGCACCA131230.12869576166992436No Hit
AACGGAATCCCAAAAGCAGCTG131190.12865653412693268No Hit
ACCCCCCACTGCTAAATTTGACTG130330.12781314195261176No Hit
GGGAATACCGGGTGCTGTAGGCTT127110.12465532474178224No Hit
TGTAAACATCCCCGACTGGAAGC127020.12456706277005097No Hit
TGTCAGTTTGTCAAATACCCCA125200.12278220956392996No Hit
TGTAAACATCCTACACTCAGCT124770.1223605134767695No Hit
AGCAGCATTGTACAGGGCTATG124420.12201727247559237No Hit
ACCACAGGGTAGAACCACGGAC121260.11891829657925038No Hit
TGTCAGTTTGTCAAATACCCCAA119840.11752571880304606No Hit
TATTGCACTTGTCCCGGCCTGTAAT119630.11731977420233977No Hit
AGAGGTAGTAGGTTGCATAGTT118150.11586835511164797No Hit
ATCCCGGACGAGCCCCCA117240.11497592850858745No Hit
TATTGCACTTGTCCCGGCCTGTTT116760.11450519799268741No Hit
GAATACCGGGTGCTGTAGGCTT115230.113004744473256No Hit
CGCGACCTCAGATCAGACGTGGCG115040.11281841364404555No Hit
CAAAGTGCTGTTCGTGCAGGT112830.11065109189375573No Hit
TGAGGTAGTAGGTTGTATAGT108450.10635567593616777No Hit
CCCCCACTGCTAAATTTGACTGGCT107190.10512000833193012No Hit
AGCTACATCTGGCTACTGGGTCTC106740.10467869847327384No Hit
AGCTACATTGTCTGCTGGGTTT106530.10447275387256758No Hit
TAGCAGCACGTAAATATTGGCGT105890.10384511318470083No Hit
CCCCCCACTGCTAAATTTGACTGTA103640.10163856389141933No Hit
TTAATGCTAATCGTGATAGGGGTTT102080.10010868971474418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)