FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6771518
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6399449.45052497829881No Hit
CCCCCCACTGCTAAATTTGACTG3700875.465347651737764No Hit
TAGCTTATCAGACTGATGTTGAC3131464.6244579132773485No Hit
TAGCTTATCAGACTGATGTTGA2910484.298120450983074No Hit
TCGTACGACTCTTAGCGG2759114.074581209117365No Hit
TTCAAGTAATCCAGGATAGGCT2492373.6806665802261764No Hit
TAGCAGCACGTAAATATTGGCG2333213.4456232708825403No Hit
AAGCTGCCAGTTGAAGAACTGT1860132.7469911473321047No Hit
CCCCCACTGCTAAATTTGACTG1692872.499985970649417No Hit
AACATTCAACGCTGTCGGTGAGT1610912.378949594463162No Hit
AACATTCAACGCTGTCGGTGAG1564392.310250079819621No Hit
CATAAAGTAGAAAGCACTACT1232361.8199168930806948No Hit
TAGCTTATCAGACTGATGTTGACT1107581.6356450651094776No Hit
TGAGAACTGAATTCCATGGGTT926671.3684819267998698No Hit
TGAGGTAGTAGGTTGTATAGTT637170.9409559274596921No Hit
TTCAAGTAATTCAGGATAGGTT554330.8186199903773422No Hit
TGAGGTAGTAGATTGTATAGTT450360.6650798240512689No Hit
TATTGCACTTGTCCCGGCCTGT446770.6597782063047015No Hit
TGAGGTAGTAGTTTGTGCTGTT428650.6330190660351194No Hit
TGTAAACATCCTTGACTGGAAGCT384430.5677161310063711No Hit
TTCACAGTGGCTAAGTTCCGC383690.5666233184346553No Hit
AGCAGCATTGTACAGGGCTATGA381710.5636993064184427No Hit
TAGCTTATCAGACTGATGTTGACA348910.5152611275640114No Hit
TTAATGCTAATCGTGATAGGGGTT321250.47441356576176863No Hit
CCCATAAAGTAGAAAGCACTAC283500.4186653568668059No Hit
CATTGCACTTGTCTCGGTCTGA280660.41447131942941No Hit
CCCATAAAGTAGAAAGCACT277450.4097308757061563No Hit
CAACACCAGTCGATGGGCTGT254700.3761342729946225No Hit
TAGCTTATCAGACTGATGTTG253100.3737714350017234No Hit
ACTGGACTTGGAGTCAGAAGGC240820.3556366534062229No Hit
TGTAAACATCCTACACTCTCAGCT221600.3272530620165227No Hit
CCCCCACTGCTAAATTTGACTGGCTT210610.3110233185527972No Hit
TAGCAGCACGTAAATATTGGC200490.2960783682477105No Hit
TGTAAACATCCCCGACTGGAAGCT198580.29325773039368724No Hit
TATTGCACTTGTCCCGGCCTGTA198270.292799930532563No Hit
TGAGGTAGTAGTTTGTACAGTT162360.23976898532943425No Hit
CAACGGAATCCCAAAAGCAGCTGT161230.2381002309969493No Hit
CAAAGAATTCTCCTTTTGGGCT155790.23006658182109238No Hit
AACGGAATCCCAAAAGCAGCTG153620.226861982793223No Hit
TGAGAACTGAATTCCATAGGCTGT152300.2249126414490813No Hit
CCCATAAAGTAGAAAGCACTA147870.21837053375624196No Hit
TTCACAGTGGCTAAGTTCTGC144990.2141174253690236No Hit
TTCTCACTACTGCACTTGACTAG141140.20843184644861018No Hit
CAACGGAATCCCAAAAGCAGCT140790.2079149756376635No Hit
TTCACAGTGGCTAAGTTCCG139990.20673355664121398No Hit
AACATTCAACGCTGTCGGTGAGTT135400.19995516514908473No Hit
TGATAACTGAATTCCATGGGTT124680.18412415059666087No Hit
TATTGCACTTGTCCCGGCCTGTAT121280.17910311986175034No Hit
TCAGTGCATCACAGAACTTTGT114660.1693268776661304No Hit
TTATAAAGCAATGAGACTGATT111810.1651180724912789No Hit
ATCACATTGCCAGGGATTTCC111670.16491132416690024No Hit
CACCCGGCTGTGTGCACATGTGC107170.15826584231187157No Hit
TGAGGTAGTAAGTTGTATTGTT103490.1528313149282037No Hit
AGTGTTTCCTACTTTATGGATG101760.15027649634838158No Hit
AGCAGCATTGTACAGGGCTATGT101010.14916891603921012No Hit
CTTCTCACTACTGCACTTGACTAG100590.1485486710660741No Hit
TAGCAGCACGTAAATATTGGCGT99520.14696852315832284No Hit
ACCGGGTGCTGTAGGCTT96160.14200656337323478No Hit
TTCAAGTAATCCAGGATAGGC95980.14174074409903364No Hit
CTCCCACTGCTTCACTTGACTAGCCT95620.14120910555063135No Hit
TGTCAGTTTGTCAAATACCCCA95540.1410909636509864No Hit
AGCAGCATTGTACAGGGCTATG94750.13992431239199246No Hit
TTCAAGTAATCCAGGATAGGCTT94320.13928929968140083No Hit
TGTAAACATCCTACACTCAGCT93870.13862475149589798No Hit
TGATGTAGTAGGTTGTATAGTT93570.1381817193722294No Hit
ACTGGACTTGGAGTCAGAAGG93140.13754670666163776No Hit
TATTGCACTTGTCCCGGCCTGTAA93060.1374285647619928No Hit
CCCCCCACTGCTAAATTTGACTGG92240.13621761029063204No Hit
CACTAGATTGTGAGCTCCTGGA90140.133116385424952No Hit
TTCACAGTGGCTAAGTTCTG88520.13072401195714167No Hit
TGAGAACTGAATTCCATGGGTTG87200.12877467061299994No Hit
TTCACAGTGGCTAAGTTCCGCT85530.1263084584579115No Hit
TTCACCACCTTCTCCACCCAGC84470.12474307828761586No Hit
TGTAAACATCCCCGACTGGA80820.11935285411631483No Hit
CAAAGAATTCTCCTTTTGGGCTT80560.11896889294246873No Hit
ACTGGACTTGGAGTCAGAAGGCT80470.11883598330536815No Hit
CTCGTACGACTCTTAGCGG80010.11815666738240968No Hit
TCAGTGCATCACAGAACTTTGTT77910.11505544251672964No Hit
TATTGCACTTGTCCCGGCCTGTT77600.11459764265560543No Hit
ACCCCCCACTGCTAAATTTGACTG76150.11245632072454065No Hit
TGAGGTAGTAGGTTGTGTGGTT72680.10733191582744075No Hit
CACTAGATTGTGAGCTCCTGGAA71440.10550071638294398No Hit
TCGACCGGACCTCGACCGGCT70050.1034480008766129No Hit
TGTAAACATCCCCGACTGGAAGC69330.10238472377980831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)