FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9451609
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG110828011.725834193945179No Hit
CAACGGAATCCCAAAAGCAGCTG6737067.127950383897599No Hit
GCCTGTCTGAGCGTCGCT5774016.10902334195162No Hit
CCCCCACTGCTAAATTTGACTG3921314.148827993202004No Hit
TTCAAGTAATCCAGGATAGGCT3655093.8671616652783665No Hit
AAGCTGCCAGTTGAAGAACTGT2943373.1141470198354586No Hit
TAGCAGCACGTAAATATTGGCG2616992.7688301536807116No Hit
AACATTCAACGCTGTCGGTGAGT1849641.9569578047504927No Hit
AACATTCAACGCTGTCGGTGAG1467951.5531218018011537No Hit
TGAGGTAGTAGGTTGTATAGTT1323611.4004070629667393No Hit
TAGCTTATCAGACTGATGTTGA1317871.3943340229160983No Hit
TAGCTTATCAGACTGATGTTGACT1168411.2362022169981852No Hit
TAGCTTATCAGACTGATGTTGAC1101381.1652830750827716No Hit
AACCGGGCGGAAACACCA1055231.1164554098672512No Hit
TATTGCACTTGTCCCGGCCTGTAT1043011.103526394289057No Hit
TATTGCACTTGTCCCGGCCTGTAA1006381.064771088181917No Hit
TATTGCACTTGTCCCGGCCTGT918810.972120196677624No Hit
CATAAAGTAGAAAGCACTACT855230.9048512269180835No Hit
TGAGGTAGTAGATTGTATAGTT827390.8753959246515594No Hit
TGTAAACATCCTTGACTGGAAGCT652820.6906972135643783No Hit
TTCAAGTAATTCAGGATAGGTT623560.6597395215989151No Hit
TTAATGCTAATCGTGATAGGGGTT552950.5850326648087114No Hit
TATTGCACTTGTCCCGGCCTGTA520460.5506575652886191No Hit
CCCATAAAGTAGAAAGCACTAC502870.5320469773982398No Hit
TCGTACGACTCTTAGCGG424610.4492462606102305No Hit
CAACGGAATCCCAAAAGCAGCTGT422590.4471090583624439No Hit
AGCAGCATTGTACAGGGCTATGA413040.4370049586266212No Hit
TGAGAACTGAATTCCATGGGTT386550.4089779845950039No Hit
TTCTCACTACTGCACTTGACTAG386450.40887218250352925No Hit
ACTGGACTTGGAGTCAGAAGGC379020.4010110871069677No Hit
GGAATACCGGGTGCTGTAGGCTTT365940.38717217354209216No Hit
TGAGGTAGTAGTTTGTACAGTT332300.3515803499700421No Hit
TGTAAACATCCCCGACTGGAAGCT330660.349845195669859No Hit
GGAATACCGGGTGCTGTAGGCTT328560.3476233517488927No Hit
CCCATAAAGTAGAAAGCACT292000.30894210710578485No Hit
CAACACCAGTCGATGGGCTGT258280.2732656418605552No Hit
CATTGCACTTGTCTCGGTCTGA253930.26866325088141074No Hit
CACCCGGCTGTGTGCACATGTGC239490.2533854288724809No Hit
CTCCCACTGCTTCACTTGACTAG230950.2443499302605514No Hit
CGCCTGTCTGAGCGTCGCT212410.22473422250116354No Hit
TGTAAACATCCTACACTCTCAGCT203480.21528609573248322No Hit
ACCGGGTGCTGTAGGCTTA202390.21413285293541026No Hit
TGATGTAGTAGGTTGTATAGTT197220.2086628848061743No Hit
ACCGGGTGCTGTAGGCTT195740.20709701385235044No Hit
CTTCTCACTACTGCACTTGACTAG194300.2055734637351164No Hit
TTCAAGTAATCCAGGATAGGC190430.20147892279505003No Hit
AGCTCGGTCTGAGGCCCCTCAGT187090.19794513293979896No Hit
TAGCAGCACGTAAATATTGGC185920.1967072484695463No Hit
CCCCCCACTGCTAAATTTGACTGG179740.190168679216417No Hit
TGAGGTAGTAGTTTGTGCTGTT179240.1896396687590441No Hit
CCCCCACTGCTAAATTTGACTGGCTT170320.1802021221995112No Hit
CAACGGAATCCCAAAAGCAGCT169600.1794403471408942No Hit
AACGGAATCCCAAAAGCAGCTG168600.17838232622614836No Hit
ACCGGGTGCTGTAGGCTTT160920.17025672560090033No Hit
ACCCCCCACTGCTAAATTTGACTG156110.16516764500097283No Hit
ATCCCGGACGAGCCCCCA151780.16058641444012337No Hit
TTCAAGTAATCCAGGATAGGCTT147770.15634375057199257No Hit
TATTGCACTTGTCCCGGCCTGTT146930.15545501300360606No Hit
TGAGAACTGAATTCCATAGGCTGT146350.15484136087305347No Hit
CAAAGAATTCTCCTTTTGGGCT145100.15351883472962116No Hit
TTCACAGTGGCTAAGTTCCGC137670.1456577393330596No Hit
CACTAGATTGTGAGCTCCTGGA136610.144536237163429No Hit
TTCACAGTGGCTAAGTTCCG136550.14447275590854425No Hit
TGAGGTAGTAGGTTGTATAGT133410.1411505702362423No Hit
TAGCTTATCAGACTGATGTTGACA130190.13774374289076072No Hit
TATTGCACTTGTCCCGGCCTGTAG127240.13462258119226048No Hit
TCGTTTCCCGGCCAATGCACCA127100.13447445826419607No Hit
TTCACAGTGGCTAAGTTCTGC126620.13396660822511808No Hit
TGATGTAGTAGATTGTATAGTT121620.12867650365138888No Hit
CTACGCCTGTCTGAGCGTCGCT120480.12747035980857863No Hit
CCCATAAAGTAGAAAGCACTA114890.12155602289514938No Hit
CCCCCCACTGCTAAATTTGACTGT110290.11668912668731853No Hit
TGAGGTAGTAGGTTGTGTGGTT110170.11656216417754903No Hit
TGTAAACATCCCCGACTGGAAGC108650.11495397238713535No Hit
TTATAAAGCAATGAGACTGATT108390.11467888694930144No Hit
AACATTCAACGCTGTCGGTGAGTT108090.11436148067487768No Hit
CCCCCCACTGCTAAATTTGACTGGCT106560.11274270867531655No Hit
ACTGGACTTGGAGTCAGAAGGCT106430.11260516595639959No Hit
CACTAGATTGTGAGCTCCTGGAA105590.11171642838801309No Hit
AGCAGCATTGTACAGGGCTATGT105300.11140960232273679No Hit
GACCCGCGGGCGCTCTCCAGTCCT101720.1076218874479467No Hit
CAAAGAATTCTCCTTTTGGGCTT98000.10368604964509218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)