FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581273
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG100960510.537274117959065No Hit
CAACGGAATCCCAAAAGCAGCTG4235964.421082668242518No Hit
AAGCTGCCAGTTGAAGAACTGT3349553.495934204150117No Hit
TTCAAGTAATCCAGGATAGGCT3114193.2502883489490384No Hit
CCCCCACTGCTAAATTTGACTG3032443.1649656574862233No Hit
TAGCAGCACGTAAATATTGGCG2440122.5467597051039044No Hit
AACATTCAACGCTGTCGGTGAGT2435512.541948235897255No Hit
GCCTGTCTGAGCGTCGCT1911571.9951106705758201No Hit
TAGCTTATCAGACTGATGTTGAC1648181.720209830155137No Hit
AACCGGGCGGAAACACCA1546691.6142844484235026No Hit
TAGCTTATCAGACTGATGTTGA1506461.5722962908999671No Hit
TAGCTTATCAGACTGATGTTGACT1310401.3676679497599118No Hit
AACATTCAACGCTGTCGGTGAG1266901.3222668845778633No Hit
TGAGGTAGTAGGTTGTATAGTT1136041.1856879560784876No Hit
TTAATGCTAATCGTGATAGGGGTT903640.9431314607150845No Hit
TATTGCACTTGTCCCGGCCTGT877870.9162352434796504No Hit
TATTGCACTTGTCCCGGCCTGTAT874190.9123944177355138No Hit
TATTGCACTTGTCCCGGCCTGTAA856410.8938373846565065No Hit
TGTAAACATCCTTGACTGGAAGCT835690.8722118657927814No Hit
TGAGGTAGTAGATTGTATAGTT750070.7828500450827358No Hit
TTCAAGTAATTCAGGATAGGTT749010.7817437202760008No Hit
CGCCTGTCTGAGCGTCGCT646030.6742632215990506No Hit
AGCAGCATTGTACAGGGCTATGA596590.6226625626886949No Hit
TCGTACGACTCTTAGCGG576070.6012457843545425No Hit
TATTGCACTTGTCCCGGCCTGTA533570.5568884218203572No Hit
CATAAAGTAGAAAGCACTACT523560.5464409583152469No Hit
ACTGGACTTGGAGTCAGAAGGC475080.49584225394683984No Hit
TTCTCACTACTGCACTTGACTAG449950.46961400640603806No Hit
TGTAAACATCCCCGACTGGAAGCT417180.4354118706355617No Hit
ACCGGGTGCTGTAGGCTT301410.3145824150924412No Hit
TGTAAACATCCTACACTCTCAGCT298320.311357373910544No Hit
TGAGGTAGTAGTTTGTACAGTT298310.3113469368840654No Hit
CCCATAAAGTAGAAAGCACTAC291120.3038427148459292No Hit
CAACGGAATCCCAAAAGCAGCTGT276600.28868815239895573No Hit
TTCACAGTGGCTAAGTTCCG274280.28626676225591313No Hit
TACGCCTGTCTGAGCGTCGCT273460.2854109260846654No Hit
CTACGCCTGTCTGAGCGTCGCT264020.27555837308883696No Hit
AGCTCGGTCTGAGGCCCCTCAGT253000.2640567699093847No Hit
ACCGGGTGCTGTAGGCTTA242800.25341100290118024No Hit
CTTCTCACTACTGCACTTGACTAG241570.2521272486443085No Hit
GGAATACCGGGTGCTGTAGGCTTT241290.25183501190290686No Hit
CTCCCACTGCTTCACTTGACTAG239260.2497162955277446No Hit
ACCGGGTGCTGTAGGCTTT229880.23992636469078796No Hit
AACATTCAACGCTGTCGGTGAGTT221450.23112795136930134No Hit
CCCATAAAGTAGAAAGCACT221420.23109664028986543No Hit
CATTGCACTTGTCTCGGTCTGA218330.2278715991079682No Hit
CCCCCCACTGCTAAATTTGACTGG215100.22450043955537013No Hit
GGAATACCGGGTGCTGTAGGCTT213110.22242347128612244No Hit
ACGCCTGTCTGAGCGTCGCT200970.20975292114106342No Hit
TGAGGTAGTAGTTTGTGCTGTT198370.20703929425661913No Hit
TAGCTTATCAGACTGATGTTGACA194210.20269749124150832No Hit
TCCTGTCTGAGCGTCGCT192210.20061008594578195No Hit
CAACACCAGTCGATGGGCTGT182450.1904235481026373No Hit
CAAAGAATTCTCCTTTTGGGCT177410.18516328675740687No Hit
TTCAAGTAATCCAGGATAGGCTT171640.17914112247923633No Hit
ATCCCGGACGAGCCCCCA169130.17652142883309974No Hit
GCGTTCGCGCTTTCCCCTG166440.1737138687103478No Hit
CCCCCCACTGCTAAATTTGACTGGCT156220.16304722764918606No Hit
TTCAAGTAATCCAGGATAGGC152940.15962388296419486No Hit
TATTGCACTTGTCCCGGCCTGTT147620.15407138487756272No Hit
CCCCCACTGCTAAATTTGACTGGCTT145650.15201529066127226No Hit
AGCAGCATTGTACAGGGCTATGT139360.14545040100621284No Hit
CACCCGGCTGTGTGCACATGTGC136350.14230885603614468No Hit
TGAGAACTGAATTCCATGGGTT131070.13679810605542708No Hit
TGATGTAGTAGGTTGTATAGTT130300.13599445501657242No Hit
TGTAAACATCCTACACTCAGCT128470.1340844791709828No Hit
TGAGGTAGTAGGTTGTATAGT128450.13406360511802554No Hit
ACCCCCCACTGCTAAATTTGACTG127840.13342694650282902No Hit
AGCAGCATTGTACAGGGCTATG126170.1316839630808975No Hit
TGAGGTAGTAGGTTGTGTGGTT124330.12976355020882924No Hit
GACCCGCGGGCGCTCTCCAGTCCT123290.12867809945505154No Hit
AACTTAACTTGACCGCTCTGACCA121670.12698730116551318No Hit
GGGCTACGCCTGTCTGAGCGTCGCT121320.12662200523876108No Hit
CAACGGAATCCCAAAAGCAGCT120750.12602709472947907No Hit
TGTAAACATCCCCGACTGGAAGC120370.12563048772329105No Hit
TAGCAGCACGTAAATATTGGC118650.12383531916896637No Hit
CAAAGTGCTGTTCGTGCAGGT118590.12377269701009459No Hit
TCGTTTCCCGGCCAATGCACCA118350.12352220837460744No Hit
TTATAAAGCAATGAGACTGATT117520.12265593517688099No Hit
TATTGCACTTGTCCCGGCCTGTAG116260.12134086984057339No Hit
CACTAGATTGTGAGCTCCTGGA115760.12081901851664179No Hit
CCCCCCACTGCTAAATTTGACTGTA113570.11853330971782143No Hit
CCCCCCACTGCTAAATTTGACTGA113410.11836631729416332No Hit
CAAAGAATTCTCCTTTTGGGCTT110700.1155378831184541No Hit
AACGGAATCCCAAAAGCAGCTG110040.11484903937086438No Hit
GGGAATACCGGGTGCTGTAGGCTT109500.11428543994101827No Hit
TAGCTTATCAGACTGATGTTG108600.11334610755794142No Hit
GGGAATACCGGGTGCTGTAGGCTTT105700.1103193698791382No Hit
CCCCCCACTGCTAAATTTGACTGT105090.10968271126394165No Hit
TTCACAGTGGCTAAGTTCTG105010.1095992150521126No Hit
TGTCAGTTTGTCAAATACCCCA104500.10906692670170237No Hit
AGAGGTAGTAGGTTGCATAGTT103440.1079606018949674No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT102000.10645767008204442No Hit
CCCCCACAACCGCGCTTGACTAG101700.10614455928768546No Hit
TCGTTTCCCGGGCGGCGCACCA100540.10493386421616417No Hit
ACCACAGGGTAGAACCACGGAC99900.10426589452153173No Hit
CACTAGATTGTGAGCTCCTGGAA99760.10411977615083089No Hit
AATGACACGATCACTCCCGTTG98220.1025124740731216No Hit
TGTCAGTTTGTCAAATACCCCAA97620.1018862524844037No Hit
CAAAGTGCTGTTCGTGCAGGTAG96090.10028938743317302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)