FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7450534
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG104833414.070588765852218No Hit
CAACGGAATCCCAAAAGCAGCTG4917386.600036990637181No Hit
CCCCCACTGCTAAATTTGACTG3158094.238743155859701No Hit
TTCAAGTAATCCAGGATAGGCT2470323.3156281147096305No Hit
GCCTGTCTGAGCGTCGCT2127682.855741615298984No Hit
TAGCAGCACGTAAATATTGGCG1997992.6816735552109416No Hit
AACATTCAACGCTGTCGGTGAGT1860032.4965056195971993No Hit
AAGCTGCCAGTTGAAGAACTGT1504542.0193720342729797No Hit
AACATTCAACGCTGTCGGTGAG1378331.8499747803311817No Hit
TAGCTTATCAGACTGATGTTGACT1369341.8379085311200514No Hit
TAGCTTATCAGACTGATGTTGAC1280081.7181050378402407No Hit
TAGCTTATCAGACTGATGTTGA1146201.538413219777267No Hit
TATTGCACTTGTCCCGGCCTGTAA924961.2414680612154778No Hit
TATTGCACTTGTCCCGGCCTGTAT838941.1260132495201014No Hit
TGAGGTAGTAGGTTGTATAGTT810731.0881501916506924No Hit
TTCAAGTAATTCAGGATAGGTT641100.8604752357347809No Hit
TGTAAACATCCTTGACTGGAAGCT630180.845818568172429No Hit
TGAGGTAGTAGATTGTATAGTT568590.7631533524979551No Hit
AACCGGGCGGAAACACCA493750.6627041766402247No Hit
TATTGCACTTGTCCCGGCCTGT436640.586051952786203No Hit
AGCAGCATTGTACAGGGCTATGA418790.5620939385015893No Hit
CCCCCACTGCTAAATTTGACTGGCTT383210.5143389722132669No Hit
TATTGCACTTGTCCCGGCCTGTA361670.48542829279082544No Hit
GGAATACCGGGTGCTGTAGGCTTT356150.47801942786919704No Hit
TTCTCACTACTGCACTTGACTAG346750.46540288253164136No Hit
CAACGGAATCCCAAAAGCAGCT343990.4616984500708271No Hit
TGTAAACATCCCCGACTGGAAGCT343930.4616179189303747No Hit
ACTGGACTTGGAGTCAGAAGGC322290.4325730209405124No Hit
CATAAAGTAGAAAGCACTACT321720.4318079751062138No Hit
CAACGGAATCCCAAAAGCAGCTGT294460.3952199936273024No Hit
CCCATAAAGTAGAAAGCACTAC272470.3657053306514674No Hit
CTCCCACTGCTTCACTTGACTAG261560.3510620849458576No Hit
GGAATACCGGGTGCTGTAGGCTT236930.31800405179011326No Hit
TCGTTTCCCGGCCAATGCACCA235650.316286054127127No Hit
CTTCTCACTACTGCACTTGACTAG222450.29856920322758074No Hit
TAGCTTATCAGACTGATGTTGACA218680.2935091632358164No Hit
CAAAGAATTCTCCTTTTGGGCT211780.28424808208378083No Hit
TGAGGTAGTAGTTTGTACAGTT210070.28195294458088505No Hit
CCCCCCACTGCTAAATTTGACTGGCT205750.2761547024683063No Hit
TCCTGTCTGAGCGTCGCT184260.2473111323295753No Hit
TGTAAACATCCTACACTCTCAGCT172870.2320236375003456No Hit
CCCCCCACTGCTAAATTTGACTGG169940.22809103347491605No Hit
ACCCCCCACTGCTAAATTTGACTG168710.22644014509564012No Hit
CTCCCACTGCTTCACTTGACTAGCCT164880.22129957396342329No Hit
CACCCGGCTGTGTGCACATGTGC160520.21544764442387618No Hit
CCCATAAAGTAGAAAGCACT155770.20907226247138797No Hit
CATTGCACTTGTCTCGGTCTGA150280.20170366311998578No Hit
TAGCAGCACGTAAATATTGGC142760.19161042684994123No Hit
GGGAATACCGGGTGCTGTAGGCTTT142470.1912211930044209No Hit
AACATTCAACGCTGTCGGTGAGTT133540.179235474933743No Hit
TTCAAGTAATCCAGGATAGGCTT129610.17396068523410538No Hit
TCGTACGACTCTTAGCGG127670.17135684502614176No Hit
AGCTCGGTCTGAGGCCCCTCAGT126110.1692630353743772No Hit
TTCAAGTAATCCAGGATAGGC121200.1626729037140157No Hit
CACTAGATTGTGAGCTCCTGGAA121050.16247157586288447No Hit
TGAGGTAGTAGTTTGTGCTGTT120920.1622970917252374No Hit
GGGAATACCGGGTGCTGTAGGCTT119740.16071331262967192No Hit
AACGGAATCCCAAAAGCAGCTG114500.15368025969682172No Hit
CAACACCAGTCGATGGGCTGT113460.15228438659564536No Hit
CAAAGAATTCTCCTTTTGGGCTT112380.15083482606750065No Hit
CACTAGATTGTGAGCTCCTGGA111690.1499087179522971No Hit
TATTGCACTTGTCCCGGCCTGTAG108610.1457747860757363No Hit
AGCAGCATTGTACAGGGCTATGT99750.133883021002253No Hit
TGATGTAGTAGGTTGTATAGTT99600.13368169315112177No Hit
CGCCTGTCTGAGCGTCGCT98840.13266163203872366No Hit
GACCCGCGGGCGCTCTCCAGTCCT98490.13219186705275085No Hit
TGTAAACATCCCCGACTGGAAGC97960.13148050864542057No Hit
CAACGGAATCCCAAAAGCAGCTGA96710.1298027765526605No Hit
TATTGCACTTGTCCCGGCCTGTT93140.12501117369573778No Hit
TGTCAGTTTGTCAAATACCCCAA92680.12439376828560207No Hit
TTCACAGTGGCTAAGTTCCG92610.12429981528840751No Hit
CCCCCCACTGCTAAATTTGACTGTA91840.12326633231926731No Hit
CTACGCCTGTCTGAGCGTCGCT87000.11677015365610034No Hit
TGTCAGTTTGTCAAATACCCCA85600.11489109371220908No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC84780.11379050145935848No Hit
AATGCACCCGGGCAAGGATTCT84020.11277044034696036No Hit
TTCACAGTGGCTAAGTTCTGC82560.11081084926261663No Hit
ACTGGACTTGGAGTCAGAAGGCT82000.1100592252850601No Hit
CCCCCACAACCGCGCTTGACTAG81680.10962972586931352No Hit
ATCACATTGCCAGGGATTTCC79980.10734801022315986No Hit
CCCCCCACTGCTAAATTTGACTGA78350.10516024757420073No Hit
CCCCCCACTGCTAAATTTGACTGT77160.10356304662189314No Hit
TATTGCACTTGTCCCGGCCTGTAAA76320.10243561065555837No Hit
TATTGCACTTGTCCCGGCCTGTAAT75970.10196584566958557No Hit
CCCCCACTGCTAAATTTGACTGGCT75920.10189873638587515No Hit
CTACGGGGATGATTTTACGAACTGAA75410.10121422169202905No Hit
TGAGGTAGTAGGTTGTATAGT75280.10103973755438202No Hit
ATGCACCTGGGCAAGGATTCTGA74510.1000062545852418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)