FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8446655
Sequences flagged as poor quality0
Sequence length18-26
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT331426739.23762720272108No Hit
CAACGGAATCCCAAAAGCAGCTG5384026.374144557816082No Hit
CCCCCCACTGCTAAATTTGACTG4753675.627872808821953No Hit
TTCAAGTAATCCAGGATAGGCT2484312.9411761223821737No Hit
CCCCCACTGCTAAATTTGACTG2208532.6146800123836007No Hit
TAGCAGCACGTAAATATTGGCG1197351.4175433943969535No Hit
AACCGGGCGGAAACACCA757140.8963785072315609No Hit
AACATTCAACGCTGTCGGTGAGT736490.8719309596520752No Hit
TAGCTTATCAGACTGATGTTGA656630.7773846570032753No Hit
TAGCTTATCAGACTGATGTTGAC549090.6500679854924819No Hit
TGAGGTAGTAGGTTGTATAGTT535110.6335170549762006No Hit
CATAAAGTAGAAAGCACTACT529260.6265912364125207No Hit
AACATTCAACGCTGTCGGTGAG506660.5998350826451418No Hit
CGCCTGTCTGAGCGTCGCT483150.5720015793234126No Hit
AAGCTGCCAGTTGAAGAACTGT461230.5460504779702734No Hit
TTCAAGTAATTCAGGATAGGTT396290.4691679724103802No Hit
TCGTACGACTCTTAGCGG376900.4462121395984564No Hit
TAGCTTATCAGACTGATGTTGACT362280.4289035126923024No Hit
CAACACCAGTCGATGGGCTGT354700.419929546074748No Hit
CATTGCACTTGTCTCGGTCTGA350600.41507555357712606No Hit
TGAGGTAGTAGATTGTATAGTT338210.4004070250294347No Hit
CCCATAAAGTAGAAAGCACTAC298330.3531930687354935No Hit
TCCTTCTGATCGAGGCCC291170.34471634037379295No Hit
GCCGGTCTGAGCGTCGCT285760.33831143807815045No Hit
CAACGGAATCCCAAAAGCAGCTGT278050.32918356438140306No Hit
GCCTGTCTGAGCGTCGCTT274090.3244953179690659No Hit
CCCATAAAGTAGAAAGCACT232380.27511482356033246No Hit
CACCCGGCTGTGTGCACATGTGC228630.27067519627592224No Hit
CTCCCACTGCTTCACTTGACTAG224420.26569097471129105No Hit
TATTGCACTTGTCCCGGCCTGTAA209950.24855993289651349No Hit
TCCTGTCTGAGCGTCGCT208460.24679592098884115No Hit
AGCAGCATTGTACAGGGCTATGA204600.24222606463742158No Hit
ACTGGACTTGGAGTCAGAAGGC184230.21811000922850524No Hit
TTCTCACTACTGCACTTGACTAG183780.21757725395437602No Hit
GCCTGTCTGAGCGTCGCG157560.18653537998177977No Hit
TATTGCACTTGTCCCGGCCTGTAT152690.18076978401509236No Hit
CAACGGAATCCCAAAAGCAGCT151980.17992921458257735No Hit
CTACGCCTGTCTGAGCGTCGCT142440.16863480277103776No Hit
TGTAAACATCCTACACTCTCAGCT137950.16331908903583725No Hit
CTTCTCACTACTGCACTTGACTAG133650.15822831641638022No Hit
TCAAACGAGAACTTTGAAGGCCGAA130860.15492523371677902No Hit
GCCTGTCTGAGCGTCGCA124420.1473009137936852No Hit
AACGGAATCCCAAAAGCAGCTG122950.1455605798981964No Hit
TGATGTAGTAGGTTGTATAGTT121450.1437847289844323No Hit
NCCTGTCTGAGCGTCGCT121340.14365449991742293No Hit
CCCCCACTGCTAAATTTGACTGGCTT121140.1434177197955877No Hit
CCCCCCACTGCTAAATTTGACTGG120740.1429441595519173No Hit
ACCGGGTGCTGTAGGCTT119390.1413458937295296No Hit
GGAATACCGGGTGCTGTAGGCTTT113000.1337807688368946No Hit
TATTGCACTTGTCCCGGCCTGT110400.13070262725303686No Hit
ACCGGGTGCTGTAGGCTTT109320.1294240145951267No Hit
TTCAAGTAATCCAGGATAGGCTT105270.12462921712796367No Hit
ACGCCTGTCTGAGCGTCGCT101470.12013039481309465No Hit
CGCGACCTCAGATCAGACGTGGC101120.11971602959988303No Hit
TTCACAGTGGCTAAGTTCCGC100650.11915959631357029No Hit
ACCCCCCACTGCTAAATTTGACTG97730.1157026065347762No Hit
TACGCCTGTCTGAGCGTCGCT95860.11348871239561696No Hit
GACCCGCGGGCGCTCTCCAGTCCT94820.11225745576207385No Hit
AGCTCGGTCTGAGGCCCCTCAGT93180.11031585876302512No Hit
TTCAAGTAATCCAGGATAGGC92610.10964103541579477No Hit
TGTAAACATCCTTGACTGGAAGCT89870.10639714774665238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)