FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8978450
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT7825848.716248350216352No Hit
CCCCCCACTGCTAAATTTGACTG7629198.497223908358347No Hit
CAACGGAATCCCAAAAGCAGCTG6281676.996385790420395No Hit
TTCAAGTAATCCAGGATAGGCT3011573.3542203832510067No Hit
CCCCCACTGCTAAATTTGACTG2839473.1625391910630456No Hit
TAGCAGCACGTAAATATTGGCG2612122.9093217648926037No Hit
AAGCTGCCAGTTGAAGAACTGT2501572.786193608028112No Hit
AACATTCAACGCTGTCGGTGAGT1758321.95837811649004No Hit
AACATTCAACGCTGTCGGTGAG1622861.8075057498788767No Hit
TAGCTTATCAGACTGATGTTGAC1559071.7364578518563896No Hit
TGAGGTAGTAGGTTGTATAGTT1528811.7027549298598308No Hit
TAGCTTATCAGACTGATGTTGA1430981.5937940290361923No Hit
TAGCTTATCAGACTGATGTTGACT1167201.300001670666986No Hit
TGAGGTAGTAGATTGTATAGTT1080081.202969332123028No Hit
TATTGCACTTGTCCCGGCCTGTAA1035061.1528270469847244No Hit
TATTGCACTTGTCCCGGCCTGTAT971741.0823026246178349No Hit
TCCTGTCTGAGCGTCGCT940541.0475527513100813No Hit
CATAAAGTAGAAAGCACTACT923741.0288412810674448No Hit
TATTGCACTTGTCCCGGCCTGT900811.0033023517422273No Hit
AACCGGGCGGAAACACCA881170.9814277520061926No Hit
TGTAAACATCCTTGACTGGAAGCT706070.786405225846332No Hit
TTCAAGTAATTCAGGATAGGTT670940.7472782050353903No Hit
TATTGCACTTGTCCCGGCCTGTA557810.6212765009550646No Hit
CCCATAAAGTAGAAAGCACTAC498390.5550958127516442No Hit
AGCAGCATTGTACAGGGCTATGA465020.5179290412042168No Hit
TCGTACGACTCTTAGCGG401970.44770533889479813No Hit
TGAGGTAGTAGTTTGTACAGTT378500.42156496945463856No Hit
CATTGCACTTGTCTCGGTCTGA369680.41174144757725445No Hit
CAACGGAATCCCAAAAGCAGCTGT364440.40590525090633683No Hit
TTCTCACTACTGCACTTGACTAG329140.36658888783698745No Hit
TGAGAACTGAATTCCATGGGTT327500.3647622919323491No Hit
TGTAAACATCCCCGACTGGAAGCT327040.3642499540566579No Hit
ACTGGACTTGGAGTCAGAAGGC321880.35850285962499095No Hit
TTAATGCTAATCGTGATAGGGGTT292460.3257355111405643No Hit
CACCCGGCTGTGTGCACATGTGC271060.30190066214101546No Hit
TGAGGTAGTAGTTTGTGCTGTT252550.28128463153439626No Hit
CTCCCACTGCTTCACTTGACTAG241940.2694674470537788No Hit
CCCATAAAGTAGAAAGCACT233730.2603233297506808No Hit
CAACACCAGTCGATGGGCTGT224770.25034387895460797No Hit
GGAATACCGGGTGCTGTAGGCTTT215560.24008598366087688No Hit
TGTAAACATCCTACACTCTCAGCT206230.2296944350082698No Hit
TAGCAGCACGTAAATATTGGC204400.22765622128541116No Hit
TAGCTTATCAGACTGATGTTGACA186000.20716270625776162No Hit
CTTCTCACTACTGCACTTGACTAG185050.2061046171666602No Hit
CAACGGAATCCCAAAAGCAGCT179220.19961129148126905No Hit
TCGTTTCCCGGCCAATGCACCA172840.1925053879010297No Hit
CCCCCACTGCTAAATTTGACTGGCTT169730.18904153835016066No Hit
AACGGAATCCCAAAAGCAGCTG168830.18803913815859083No Hit
CCCCCCACTGCTAAATTTGACTGG166610.18556655101938532No Hit
TTCACAGTGGCTAAGTTCCGC164460.1831719283395241No Hit
TTCAAGTAATCCAGGATAGGC156580.17439535777333504No Hit
ACCGGGTGCTGTAGGCTTT155350.1730254108448563No Hit
CAAAGAATTCTCCTTTTGGGCT154340.17190049507431684No Hit
TGAGGTAGTAGGTTGTATAGT152090.1693944945953923No Hit
TGAGAACTGAATTCCATAGGCTGT151760.16902694785848338No Hit
TTATAAAGCAATGAGACTGATT150100.1671780763940324No Hit
TGTCAGTTTGTCAAATACCCCA149830.16687735633656142No Hit
TATTGCACTTGTCCCGGCCTGTT147400.16417087581932294No Hit
ACCGGGTGCTGTAGGCTT147000.16372536462306966No Hit
GGAATACCGGGTGCTGTAGGCTT143980.16036175509135764No Hit
TTCAAGTAATCCAGGATAGGCTT140720.15673083884189365No Hit
AGCTCGGTCTGAGGCCCCTCAGT140030.1559623320283568No Hit
ACCCCCCACTGCTAAATTTGACTG139730.15562819863116684No Hit
TTCACAGTGGCTAAGTTCTGC133060.14819929943364388No Hit
TGATGTAGTAGGTTGTATAGTT132020.14704097032338545No Hit
TGAGGTAGTAAGTTGTATTGTT128520.1431427473561695No Hit
TGTCAGTTTGTCAAATACCCCAA126640.1410488447337792No Hit
TATTGCACTTGTCCCGGCCTGTAG119880.13351970551709927No Hit
CACTAGATTGTGAGCTCCTGGA117810.13121418507648872No Hit
ACCGGGTGCTGTAGGCTTA117200.13053478050220252No Hit
AACATTCAACGCTGTCGGTGAGTT112880.1257232595826674No Hit
CGCCTGTCTGAGCGTCGCT111260.12391893923784172No Hit
CTACGCCTGTCTGAGCGTCGCT111030.12366277029999612No Hit
TGAGGTAGTAGATTGTATAGT107280.11948610283512187No Hit
GACCCGCGGGCGCTCTCCAGTCCT105370.1173587868730126No Hit
TAGCAGCACGTAAATATTGGCGT105220.11719172017441762No Hit
CCCCCCACTGCTAAATTTGACTGGCT105150.11711375571507332No Hit
CAAAGAATTCTCCTTTTGGGCTT103070.11479709749455641No Hit
TGTAAACATCCCCGACTGGAAGC100740.11220199477638122No Hit
CCCATAAAGTAGAAAGCACTA100480.1119124124988166No Hit
AGCAGCATTGTACAGGGCTATGT98610.10982964765633267No Hit
TCGTTTCCCGGGCGGCGCACCA98040.10919479420167179No Hit
ATCACATTGCCAGGGATTTCC97370.10844856294794758No Hit
CACTAGATTGTGAGCTCCTGGAA96990.108025327311507No Hit
TTCACAGTGGCTAAGTTCCG96580.1075686783353474No Hit
TGAGGTAGTAGGTTGTGTGGTT95910.10682244708162321No Hit
AGAGGTAGTAGGTTGCATAGTT95710.10659969148349659No Hit
CAAAGTGCTGTTCGTGCAGGTAG93510.10414937990410372No Hit
CTCCCACTGCTTCACTTGACTAGCCT92250.10274601963590596No Hit
TGATGTAGTAGATTGTATAGTT90380.10066325479342203No Hit
TCCCACTGCTTCACTTGACTAG90330.10060756589389039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)