FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7779819
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG92128011.841920743914478No Hit
CAACGGAATCCCAAAAGCAGCTG4015275.161135496854104No Hit
TTCAAGTAATCCAGGATAGGCT2257452.90167419062063No Hit
CCCCCACTGCTAAATTTGACTG2179682.801710425396786No Hit
AACATTCAACGCTGTCGGTGAGT2084492.679355393743736No Hit
AAGCTGCCAGTTGAAGAACTGT2047002.6311666119738777No Hit
TAGCTTATCAGACTGATGTTGAC1727232.2201416254028534No Hit
TAGCAGCACGTAAATATTGGCG1653312.1251265614277144No Hit
TAGCTTATCAGACTGATGTTGACT1332531.7128033441394974No Hit
TATTGCACTTGTCCCGGCCTGTAA1193791.534470146413432No Hit
AACATTCAACGCTGTCGGTGAG1162121.4937622584792782No Hit
GCCTGTCTGAGCGTCGCT1109281.4258429405619848No Hit
TATTGCACTTGTCCCGGCCTGTAT1094661.4070507295863823No Hit
TAGCTTATCAGACTGATGTTGA935211.2020973752731265No Hit
AACCGGGCGGAAACACCA909331.1688318198662462No Hit
TGAGGTAGTAGGTTGTATAGTT800121.0284558034062232No Hit
TGTAAACATCCTTGACTGGAAGCT687210.8833238922396524No Hit
TGAGGTAGTAGATTGTATAGTT589780.7580896162237194No Hit
TATTGCACTTGTCCCGGCCTGT588500.7564443337306433No Hit
TATTGCACTTGTCCCGGCCTGTA499030.6414416582185267No Hit
TTAATGCTAATCGTGATAGGGGTT486900.6258500358427361No Hit
AGCAGCATTGTACAGGGCTATGA485570.6241404845022744No Hit
TTCAAGTAATTCAGGATAGGTT431990.5552699876436714No Hit
CCCATAAAGTAGAAAGCACTAC405840.5216573804609078No Hit
CCCCCACTGCTAAATTTGACTGGCTT401170.5156546701150759No Hit
CATAAAGTAGAAAGCACTACT400940.5153590334171013No Hit
TGTAAACATCCCCGACTGGAAGCT361350.4644709600570399No Hit
TTCTCACTACTGCACTTGACTAG345770.4444447872116305No Hit
CAACGGAATCCCAAAAGCAGCTGT332240.42705363710903815No Hit
ACTGGACTTGGAGTCAGAAGGC320260.411654821275405No Hit
TGTAAACATCCTACACTCTCAGCT317830.40853135529245604No Hit
CCCCCCACTGCTAAATTTGACTGGCT296630.38128136400088486No Hit
CTCCCACTGCTTCACTTGACTAG296560.38119138761454474No Hit
CCCCCCACTGCTAAATTTGACTGG275810.3545198159494456No Hit
TAGCTTATCAGACTGATGTTGACA271600.3491083789995628No Hit
CTTCTCACTACTGCACTTGACTAG250140.3215241897015856No Hit
GGAATACCGGGTGCTGTAGGCTTT237430.30518704869612007No Hit
CAAAGAATTCTCCTTTTGGGCT234710.3016908233983336No Hit
ACCCCCCACTGCTAAATTTGACTG210490.2705589937246612No Hit
CTACGCCTGTCTGAGCGTCGCT209540.26933788562433136No Hit
CGCCTGTCTGAGCGTCGCT206560.2655074623201388No Hit
AACATTCAACGCTGTCGGTGAGTT205860.26460769845673787No Hit
GACCCGCGGGCGCTCTCCAGTCCT200590.2578337619422766No Hit
GGGCTACGCCTGTCTGAGCGTCGCT197950.2544403668003073No Hit
TGAGGTAGTAGTTTGTACAGTT191610.24629107695179026No Hit
AGCTCGGTCTGAGGCCCCTCAGT188050.24171513501792266No Hit
CATTGCACTTGTCTCGGTCTGA183250.23554532566888767No Hit
GTCAGGATGGCCGAGCGGTCTAAGG171500.2204421465332291No Hit
AACTTAACTTGACCGCTCTGACCA164350.21125170135706242No Hit
TACGCCTGTCTGAGCGTCGCT149260.1918553632160337No Hit
TATTGCACTTGTCCCGGCCTGTAG145350.1868295393504656No Hit
TGAGGTAGTAGTTTGTGCTGTT141590.18199652202705488No Hit
TCGTTTCCCGGCCAATGCACCA138680.1782560751092024No Hit
CTCCCACTGCTTCACTTGACTAGCCT136120.17496551012305042No Hit
CACCCGGCTGTGTGCACATGTGC133970.17220194968546185No Hit
TTCAAGTAATCCAGGATAGGCTT131020.16841008768970075No Hit
GGAATACCGGGTGCTGTAGGCTT126760.1629343818924322No Hit
TATTGCACTTGTCCCGGCCTGTT124350.15983662344843755No Hit
TCGTTTCCCGGGCGGCGCACCA123660.15894971335451377No Hit
CAAAGAATTCTCCTTTTGGGCTT122240.1571244780887576No Hit
TGTCAGTTTGTCAAATACCCCA121630.15634039815065107No Hit
CACTAGATTGTGAGCTCCTGGAA120570.1549778985860725No Hit
TTCACAGTGGCTAAGTTCCG117170.1506076169638394No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT116310.14950219278880397No Hit
TAGCAGCACGTAAATATTGGC113140.14542754786454543No Hit
CCCATAAAGTAGAAAGCACT112850.1450547885497079No Hit
ATCACATTGCCAGGGATTTCCA112650.1447977131601648No Hit
TAGCAGCACGTAAATATTGGCGT112310.14436068499794147No Hit
GGGAATACCGGGTGCTGTAGGCTTT111180.1429082090470228No Hit
TTCAAGTAATCCAGGATAGGC111090.1427925251217284No Hit
TGTAAACATCCTACACTCAGCT108280.13918061589864753No Hit
TATTGCACTTGTCCCGGCCTGTAAT106280.13660986200321626No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC106070.136339932844196No Hit
CACTAGATTGTGAGCTCCTGGA104350.13412908449412514No Hit
CAAAGTGCTGTTCGTGCAGGTAG104300.13406481564673933No Hit
TAGCAGCACATCATGGTTTACA101540.13051717527104423No Hit
AGCAGCATTGTACAGGGCTATGT101070.12991304810561788No Hit
TATTGCACTTGTCCCGGCCTGTTT100680.12941175109600878No Hit
TCCCACTGCTTCACTTGACTAG98100.12609547857090248No Hit
TGTCAGTTTGTCAAATACCCCAA96400.12391033775978594No Hit
ACCGGGTGCTGTAGGCTT94870.12194371102978102No Hit
TATTGCACTTGTCCCGGCCTGTAAA92980.1195143485985985No Hit
CCCCCACTGCTAAATTTGACTGGCT92700.11915444305323813No Hit
ACGCCTGTCTGAGCGTCGCT92170.11847319327094885No Hit
ACCGGGTGCTGTAGGCTTT91550.11767625956336517No Hit
ATCACATTGCCAGGGATTTCC90980.11694359470316726No Hit
AACGGAATCCCAAAAGCAGCTG90910.11685361831682717No Hit
CCCCCCACTGCTAAATTTGACTGTA86640.11136505875008146No Hit
TTATAAAGCAATGAGACTGATT85370.10973263002648263No Hit
TGTAAACATCCCCGACTGGAAGC85010.10926989432530498No Hit
GTGCGGTAACGCGACCGATCCCGGA84970.10921847924739637No Hit
GAATACCGGGTGCTGTAGGCTTT84320.10838298423138121No Hit
AGCTACATCTGGCTACTGGGTCTC82200.1056579851022241No Hit
CAACGGAATCCCAAAAGCAGCT82120.10555515494640685No Hit
TGAGGGGCAGAGAGCGAGACTTT81190.10435975438503131No Hit
TTCACAGTGGCTAAGTTCTGC80900.1039869950701938No Hit
ACCGGGTGCTGTAGGCTTA80570.10356282067744765No Hit
CGCGACCTCAGATCAGACGTGGCG79810.10258593419718376No Hit
AGAGGTAGTAGGTTGCATAGTT79430.10209749095705183No Hit
CCCCCCACTGCTAAATTTGACTGT79410.10207178341809751No Hit
TTAATGCTAATCGTGATAGGGGTTT79090.10166046279482852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)