FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8503822
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG96596611.359198252268216No Hit
CAACGGAATCCCAAAAGCAGCTG6140587.220964879086134No Hit
CCCCCACTGCTAAATTTGACTG3066093.6055434838593756No Hit
TTCAAGTAATCCAGGATAGGCT2758523.243859055375336No Hit
GCCTGTCTGAGCGTCGCT2757563.2427301512190634No Hit
AACATTCAACGCTGTCGGTGAGT2390372.8109360708631956No Hit
TAGCAGCACGTAAATATTGGCG2037352.3958050862306384No Hit
AACATTCAACGCTGTCGGTGAG2010312.3640076191623014No Hit
AAGCTGCCAGTTGAAGAACTGT1684241.980568266833431No Hit
AACCGGGCGGAAACACCA1348831.5861456178174942No Hit
TATTGCACTTGTCCCGGCCTGTAA1300581.5294064245465155No Hit
TAGCTTATCAGACTGATGTTGAC1290491.517541171487362No Hit
TAGCTTATCAGACTGATGTTGACT1207741.4202319851003466No Hit
TAGCTTATCAGACTGATGTTGA1165751.370854187681727No Hit
TATTGCACTTGTCCCGGCCTGTAT1116111.3124804352678126No Hit
TGAGGTAGTAGGTTGTATAGTT1039541.2224385693868003No Hit
TATTGCACTTGTCCCGGCCTGT711860.8371059507125149No Hit
TGAGGTAGTAGATTGTATAGTT653350.7683015942713758No Hit
TCCTGTCTGAGCGTCGCT619630.7286488357823107No Hit
TTCAAGTAATTCAGGATAGGTT608640.7157252350766514No Hit
TGTAAACATCCTTGACTGGAAGCT594070.6985917626215601No Hit
TATTGCACTTGTCCCGGCCTGTA576930.6784361196647813No Hit
CATAAAGTAGAAAGCACTACT534660.6287290585339157No Hit
CAACGGAATCCCAAAAGCAGCT456300.5365822567781875No Hit
AGCAGCATTGTACAGGGCTATGA412590.4851818394129134No Hit
TTCTCACTACTGCACTTGACTAG389110.4575707252574195No Hit
CAACGGAATCCCAAAAGCAGCTGT350410.4120617764576916No Hit
CTCCCACTGCTTCACTTGACTAG337400.3967627732565428No Hit
TGTAAACATCCCCGACTGGAAGCT334450.39329374485966423No Hit
CCCCCACTGCTAAATTTGACTGGCTT323740.3806994078662512No Hit
CCCATAAAGTAGAAAGCACTAC321260.3777830721292144No Hit
CACCCGGCTGTGTGCACATGTGC305440.3591796723873101No Hit
ACTGGACTTGGAGTCAGAAGGC300630.35352339218765394No Hit
CATTGCACTTGTCTCGGTCTGA288070.33875356280975777No Hit
TCGTACGACTCTTAGCGG277540.3263708953456458No Hit
GGAATACCGGGTGCTGTAGGCTTT262600.3088023244136578No Hit
TCGTTTCCCGGCCAATGCACCA244590.2876236120652573No Hit
CTTCTCACTACTGCACTTGACTAG232240.2731007304715456No Hit
CCCCCCACTGCTAAATTTGACTGGCT210580.2476298304456514No Hit
TGAGGTAGTAGTTTGTACAGTT204200.24012732157375824No Hit
CCCATAAAGTAGAAAGCACT199390.23447104137410213No Hit
ACCCCCCACTGCTAAATTTGACTG198630.23357732558371988No Hit
CGCCTGTCTGAGCGTCGCT196910.23155470563706532No Hit
TAGCTTATCAGACTGATGTTGACA196300.2308373811211006No Hit
CCCCCCACTGCTAAATTTGACTGG189680.2230526462101394No Hit
CAACACCAGTCGATGGGCTGT167830.19735831723664957No Hit
TAGCAGCACGTAAATATTGGC166020.1952298625253445No Hit
CAAAGAATTCTCCTTTTGGGCT162390.19096119368443978No Hit
AGCTCGGTCTGAGGCCCCTCAGT160920.18923255919514778No Hit
GGAATACCGGGTGCTGTAGGCTT157910.1856929742885023No Hit
TGTAAACATCCTACACTCTCAGCT157040.18466990489688048No Hit
AACATTCAACGCTGTCGGTGAGTT155220.18252969076728087No Hit
AACGGAATCCCAAAAGCAGCTG153170.1801190100169077No Hit
ACCGGGTGCTGTAGGCTT152660.179519279683888No Hit
CTCCCACTGCTTCACTTGACTAGCCT150290.17673229754809072No Hit
GACCCGCGGGCGCTCTCCAGTCCT150090.17649710918220066No Hit
TATTGCACTTGTCCCGGCCTGTAG142930.16807736568333626No Hit
ACCGGGTGCTGTAGGCTTT141930.16690142385388593No Hit
TGAGGTAGTAGTTTGTGCTGTT137480.16166848271283196No Hit
TATTGCACTTGTCCCGGCCTGTT137280.1614332943469419No Hit
TGATGTAGTAGGTTGTATAGTT136340.16032790902725857No Hit
TTCAAGTAATCCAGGATAGGCTT135840.1597399381125334No Hit
TTCAAGTAATCCAGGATAGGC133710.15723518201580416No Hit
CACTAGATTGTGAGCTCCTGGA132730.15608275902294286No Hit
CACTAGATTGTGAGCTCCTGGAA126140.14833330236686515No Hit
TTCACAGTGGCTAAGTTCTGC125680.147792369125318No Hit
CAACGGAATCCCAAAAGCAGCTGA124960.14694569100811378No Hit
CTACGCCTGTCTGAGCGTCGCT124700.14663994613245668No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC124570.14648707369462813No Hit
ACCGGGTGCTGTAGGCTTA117800.13852594750924938No Hit
AATGCACCCGGGCAAGGATTCT105550.12412066009848277No Hit
TTCACAGTGGCTAAGTTCCG103050.12118080552485694No Hit
GGGAATACCGGGTGCTGTAGGCTTT101950.11988726951246158No Hit
CAAAGAATTCTCCTTTTGGGCTT98930.11633592518752156No Hit
TGTAAACATCCCCGACTGGAAGC98430.1157479542727964No Hit
CAAAGTGCTGTTCGTGCAGGT97780.11498359208365369No Hit
TATTGCACTTGTCCCGGCCTGTAAA96760.11378413141761433No Hit
TATTGCACTTGTCCCGGCCTGTAAT95880.11274930260769804No Hit
TGAGGTAGTAGGTTGTATAGT91580.10769275274106162No Hit
TGAGGTAGTAGGTTGTGTGGTT90900.10689311229703537No Hit
TTCACAGTGGCTAAGTTCCGC90460.10637569789207722No Hit
AGCAGCATTGTACAGGGCTATGT90260.10614050952618716No Hit
TTATAAAGCAATGAGACTGATT89610.10537614733704445No Hit
TTCACAGTGGCTAAGTTCTG88330.10387094179534803No Hit
TGATGTAGTAGATTGTATAGTT87630.10304778251473277No Hit
TGTCAGTTTGTCAAATACCCCAA85780.10087229013024968No Hit
GTCAGGATGGCCGAGCGGTCTAAGG85750.10083701187536616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)