FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10547295
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6433836.099981085197674No Hit
CCCCCCACTGCTAAATTTGACTG5022324.761713785382887No Hit
AAGCTGCCAGTTGAAGAACTGT4839824.588683638790799No Hit
GCCTGTCTGAGCGTCGCT4254024.033280570990002No Hit
TTCAAGTAATCCAGGATAGGCT3870623.6697750465877745No Hit
TAGCAGCACGTAAATATTGGCG3362913.1884099193205464No Hit
TAGCTTATCAGACTGATGTTGA2909692.7587073273289504No Hit
TAGCTTATCAGACTGATGTTGAC2815252.6691677818815154No Hit
AACATTCAACGCTGTCGGTGAGT2782302.637927544455711No Hit
CCCCCACTGCTAAATTTGACTG2007661.9034833101757371No Hit
CATAAAGTAGAAAGCACTACT1930331.8301659335403058No Hit
TAGCTTATCAGACTGATGTTGACT1924641.8247711854082016No Hit
AACATTCAACGCTGTCGGTGAG1514011.4354486150240418No Hit
TGAGGTAGTAGGTTGTATAGTT1424771.3508392436164913No Hit
TCCTGTCTGAGCGTCGCT1415951.342476909956534No Hit
TGAGGTAGTAGATTGTATAGTT1021720.9687033500058546No Hit
CGCCTGTCTGAGCGTCGCT996460.944754081496725No Hit
TGTAAACATCCTTGACTGGAAGCT909490.8622969206796625No Hit
TATTGCACTTGTCCCGGCCTGT858230.8136967819711121No Hit
TTCAAGTAATTCAGGATAGGTT845880.8019876186263871No Hit
TATTGCACTTGTCCCGGCCTGTAT742060.7035547976993154No Hit
AGCAGCATTGTACAGGGCTATGA717570.6803355741922456No Hit
TATTGCACTTGTCCCGGCCTGTAA709540.6727222477421936No Hit
TTCTCACTACTGCACTTGACTAG503030.4769279706313325No Hit
TGAGGTAGTAGTTTGTACAGTT488860.4634932463726481No Hit
CAACACCAGTCGATGGGCTGT455870.4322150845311523No Hit
CCCATAAAGTAGAAAGCACTAC430740.4083890703730198No Hit
TATTGCACTTGTCCCGGCCTGTA424630.4025961158761559No Hit
TGTAAACATCCCCGACTGGAAGCT393990.3735460134565308No Hit
TGATGTAGTAGGTTGTATAGTT392490.37212384786810265No Hit
CCCATAAAGTAGAAAGCACT383880.3639606173905252No Hit
CATTGCACTTGTCTCGGTCTGA380920.36115421062936043No Hit
CAACGGAATCCCAAAAGCAGCTGT377080.3575134667229844No Hit
ACTGGACTTGGAGTCAGAAGGC343140.3253346000088174No Hit
TGTAAACATCCTACACTCTCAGCT341800.32406413208315493No Hit
CTTCTCACTACTGCACTTGACTAG330990.31381505874254967No Hit
CTCCCACTGCTTCACTTGACTAG322800.30605003462973207No Hit
TGATGTAGTAGATTGTATAGTT278640.26418147970640815No Hit
CTACGCCTGTCTGAGCGTCGCT270910.2568525863740419No Hit
TTATAAAGCAATGAGACTGATT259800.2463190799157509No Hit
TGAGGTAGTAGTTTGTGCTGTT253350.24020376788550998No Hit
TTCACAGTGGCTAAGTTCCG247100.23427807793372615No Hit
TGTAAACATCCTACACTCAGCT241960.2294047905173791No Hit
TAGCTTATCAGACTGATGTTGACA238890.2264940916130629No Hit
ACGCCTGTCTGAGCGTCGCT238740.22635187505422008No Hit
TAGCTTATCAGACTGATGTTG233180.22108038127311314No Hit
TACGCCTGTCTGAGCGTCGCT224890.21322054612106706No Hit
TTCAAGTAATCCAGGATAGGCTT220800.2093427746166197No Hit
TTCACAGTGGCTAAGTTCCGC213740.20264911524708468No Hit
TGTCAGTTTGTCAAATACCCCA212840.20179581589402779No Hit
AACATTCAACGCTGTCGGTGAGTT201050.19061759436898276No Hit
TAGCAGCACGTAAATATTGGC198210.18792496085489216No Hit
TTCAAGTAATCCAGGATAGGC189870.1800177201832318No Hit
TAGCAGCACGTAAATATTGGCGT181020.1716269432115059No Hit
TGTCAGTTTGTCAAATACCCCAA172460.16351111825354273No Hit
AACCGGGCGGAAACACCA168830.16006947752954667No Hit
TAGCAGCACATAATGGTTTGT167920.15920669707256693No Hit
CCCCCCACTGCTAAATTTGACTGGCT166530.1578888236272902No Hit
GCGTTCGCGCTTTCCCCTG163480.1549970869308197No Hit
CCCCCCACTGCTAAATTTGACTGG160130.15182091711666354No Hit
TGAGGTAGTAGGTTGTATAGT158550.15032290269685258No Hit
AGCTCGGTCTGAGGCCCCTCAGT158080.14987729081247847No Hit
AGCAGCATTGTACAGGGCTATGT157930.14973507425363566No Hit
CAAAGTGCTGTTCGTGCAGGT154790.14675800762185945No Hit
CAAAGAATTCTCCTTTTGGGCT154760.14672956431009088No Hit
CACCCGGCTGTGTGCACATGTGC153000.14506089001966857No Hit
TTCACAGTGGCTAAGTTCTGC152870.14493763566867146No Hit
CCCCCACTGCTAAATTTGACTGGCTT148820.1410977885799155No Hit
ATCCCACCGCTGCCACCA147200.13956184974441313No Hit
AGCAGCATTGTACAGGGCTATG146740.13912571896396186No Hit
AACGGAATCCCAAAAGCAGCTG143420.13597799246157427No Hit
TATTGCACTTGTCCCGGCCTGTT139220.1319959288139755No Hit
CATAAAGTAGAAAGCACTAC135630.12859221250567088No Hit
CAAAGAATTCTCCTTTTGGGCTT135620.12858273140174803No Hit
TGTAAACATCCCCGACTGGAAGC134080.1271226413976285No Hit
TGATGTAGTAGTTTGTACAGTT134020.12706575477409138No Hit
TGTCAGTTTGTCAAATACCCC133890.12694250042309427No Hit
ACCACAGGGTAGAACCACGGAC133620.1266865106171772No Hit
AGAGGTAGTAGGTTGCATAGTT128960.12226831618912717No Hit
TGAGGTAGTAGGTTGTGTGGTT123520.1171105956550945No Hit
TGAGGTAGTAGATTGTATAGT116550.11050226622086516No Hit
ATCACATTGCCAGGGATTTCC111690.10589444971435805No Hit
TTCACAGTGGCTAAGTTCTG110940.10518336692014398No Hit
CAACGGAATCCCAAAAGCAGCT110660.10491789601030407No Hit
CCCTGTCTGAGCGTCGCT110630.1048894526985355No Hit
TCCCACTGCTTCACTTGACTAG108410.10278464762766189No Hit
ATCCCGGACGAGCCCCCA107330.10176068840399362No Hit
CTCCCACTGCTTCACTTGACTAGCCT107260.10169432067653365No Hit
ACCGGGTGCTGTAGGCTT107180.10161847184515083No Hit
ACTTGACTTGGAGTCAGAAGGC106070.100566069309714No Hit
TCGTACGACTCTTAGCGG105820.10032904171164264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)