FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8212873
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6680448.134108490414986No Hit
CAACGGAATCCCAAAAGCAGCTG6309977.683023955198139No Hit
CCCCCACTGCTAAATTTGACTG3192323.8869711001253764No Hit
TAGCTTATCAGACTGATGTTGA3161673.8496516383487243No Hit
TAGCTTATCAGACTGATGTTGAC2762733.363902010904101No Hit
TAGCTTATCAGACTGATGTTGACT2645003.2205538792576No Hit
TTCAAGTAATCCAGGATAGGCT2305832.807580246279225No Hit
TAGCAGCACGTAAATATTGGCG1944232.3672958293644624No Hit
AAGCTGCCAGTTGAAGAACTGT1859442.264055465121645No Hit
AACATTCAACGCTGTCGGTGAGT1855112.258783254045204No Hit
AACATTCAACGCTGTCGGTGAG1663492.0254666059002737No Hit
GCCTGTCTGAGCGTCGCT1648812.0075922274702167No Hit
TATTGCACTTGTCCCGGCCTGTAA1047301.2751932241007502No Hit
TATTGCACTTGTCCCGGCCTGTAT1029841.2539339156955185No Hit
TGAGGTAGTAGGTTGTATAGTT985451.1998846201566735No Hit
TCCTGTCTGAGCGTCGCT902281.0986167690648572No Hit
CATAAAGTAGAAAGCACTACT884011.0763712040841251No Hit
TGAGGTAGTAGATTGTATAGTT745560.90779438571618No Hit
TTCAAGTAATTCAGGATAGGTT631930.7694384169827051No Hit
TATTGCACTTGTCCCGGCCTGT569550.6934844846620665No Hit
TATTGCACTTGTCCCGGCCTGTA501890.6111016205900177No Hit
TGTAAACATCCTTGACTGGAAGCT444340.5410286997010669No Hit
CATTGCACTTGTCTCGGTCTGA408390.49725595415879437No Hit
AGCAGCATTGTACAGGGCTATGA406510.49496686482306496No Hit
CAACACCAGTCGATGGGCTGT375730.45748911495404837No Hit
TTCTCACTACTGCACTTGACTAG303230.36921306344320676No Hit
TGATGTAGTAGGTTGTATAGTT301990.3677032385621938No Hit
TAGCTTATCAGACTGATGTTGACA278060.3385660535600636No Hit
CAACGGAATCCCAAAAGCAGCT268250.32662139059011386No Hit
ACTGGACTTGGAGTCAGAAGGC261080.31789119349586925No Hit
CAACGGAATCCCAAAAGCAGCTGT256350.3121319421352309No Hit
CTCCCACTGCTTCACTTGACTAG251570.3063118107390678No Hit
CACCCGGCTGTGTGCACATGTGC251500.3062265786893332No Hit
TGTAAACATCCCCGACTGGAAGCT249860.3042297135241224No Hit
TTATAAAGCAATGAGACTGATT241430.2939653395346549No Hit
CTTCTCACTACTGCACTTGACTAG237750.28948456892003566No Hit
TGAGGTAGTAGTTTGTACAGTT235600.28686672739247276No Hit
TGATGTAGTAGATTGTATAGTT233160.28389578165886653No Hit
CCCATAAAGTAGAAAGCACT218760.2663623314277476No Hit
CCCATAAAGTAGAAAGCACTAC184220.22430640288727222No Hit
TAGCAGCACGTAAATATTGGC180810.22015438446448643No Hit
CGCCCCACTGCTAAATTTGACTG176420.21480911734541616No Hit
TTAGGTAGTAGGTTGTATAGTT169220.2060423922298567No Hit
TAGCTTATCAGACTGATGTTG161260.19635029057432155No Hit
TTATGTAGTAGGTTGTATAGTT160790.1957780182403892No Hit
TTCAAGTAATCCAGGATAGGCTT156650.19073715129894253No Hit
CAAAGAATTCTCCTTTTGGGCT153210.1865486048548419No Hit
CCCCCACTGCTAAATTTGACTGGCTT149970.18260357855284015No Hit
TCGTACGACTCTTAGCGG143940.17526144626855913No Hit
CTCCCACTGCTTCACTTGACTAGCCT140330.17086590770367446No Hit
TGAGGTAGTAGTTTGTGCTGTT137540.16746880172139517No Hit
CACTAGATTGTGAGCTCCTGGA137060.16688435338035787No Hit
AGAATTACTCTGAGACCT133670.162756686971782No Hit
TTTAAACATCCTTGACTGGAAGCT129870.1581298042719034No Hit
CCCGCCACTGCTAAATTTGACTG126320.1538073217496484No Hit
TATTGCACTTGTCCCGGCCTGTAG126130.15357597761465447No Hit
TTAGGTAGTAGATTGTATAGTT125210.15245578496099965No Hit
TTAATGCTAATCGTGATAGGGGTT124840.1520052726981167No Hit
TTCACAGTGGCTAAGTTCCGC124010.1509946641084064No Hit
TTATGTAGTAGATTGTATAGTT122150.1487299267868869No Hit
AACGGAATCCCAAAAGCAGCTG121440.14786543028243587No Hit
TTCACAGTGGCTAAGTTCTG121170.1475366780906024No Hit
TGAGAACTGAATTCCATGGGTT116900.14233752305679145No Hit
TGTAAACATCCTACACTCTCAGCT116800.142215762985742No Hit
TATTGCACTTGTCCCGGCCTGTT115200.14026760184895104No Hit
ACCCCCCACTGCTAAATTTGACTG113580.13829508869795015No Hit
ACTTGACTTGGAGTCAGAAGGC113540.13824638466953038No Hit
CAAAGAATTCTCCTTTTGGGCTT113510.13820985664821556No Hit
TGTCAGTTTGTCAAATACCCCAA112750.13728448010823985No Hit
CACTAGATTGTGAGCTCCTGGAA111140.1353241429643439No Hit
TTCACAGTGGCTAAGTTCTGC108720.13237754924494755No Hit
TGCAAGTAATCCAGGATAGGCT107120.13042938810815655No Hit
AACATTCAACGCTGTCGGTGAGTT102900.12529111310987032No Hit
CCCCCCACTGCTAAATTTGACTGG101940.12412221642779572No Hit
ATCAGCATTGTACAGGGCTATGA99630.12130955878655375No Hit
TTCAAGTAATCCAGGATAGGC99200.12078599048104116No Hit
CCCTCCACTGCTAAATTTGACTG98700.12017719012579398No Hit
TGTCAGTTTGTCAAATACCCCA97660.11891088538687986No Hit
CGACGGAATCCCAAAAGCAGCTG94880.1155259554117055No Hit
AGCAGCATTGTACAGGGCTATGT92380.11248195363546959No Hit
TAGTGGGTTATCAGAACT91510.1114226410173395No Hit
CAAGGGAATCCCAAAAGCAGCTG91150.11098430476156151No Hit
CCCCCCACTGCTAAATTTGACTGGCT88610.10789159895690581No Hit
TAGCTTATCAGACTGATGTTGACC87190.10616260594800382No Hit
TATTGCACTTGTCCCGGCCTGTAAT85840.10451884498883643No Hit
TGAGAACTGAATTCCATAGGCTGT85760.10442143693199689No Hit
CAATGGAATCCCAAAAGCAGCTG84000.1022784596815268No Hit
TGAGGTAGTAGGTTGTATAGT83310.10143831519128568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)