FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7577806
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6956699.18034850720644No Hit
CAACGGAATCCCAAAAGCAGCTG4564236.023154987076734No Hit
TAGCTTATCAGACTGATGTTGAC3014203.9776684702669876No Hit
TAGCTTATCAGACTGATGTTGACT2829983.7345638038239564No Hit
CCCCCACTGCTAAATTTGACTG2375063.1342317288143824No Hit
TAGCAGCACGTAAATATTGGCG2327113.070954838379341No Hit
AACATTCAACGCTGTCGGTGAGT2178612.8749878262916733No Hit
TTCAAGTAATCCAGGATAGGCT2174062.8689834498270343No Hit
AAGCTGCCAGTTGAAGAACTGT2034372.684642494146723No Hit
TAGCTTATCAGACTGATGTTGA1639752.1638849028333533No Hit
AACATTCAACGCTGTCGGTGAG1376751.81681874674543No Hit
TGTAAACATCCTTGACTGGAAGCT1138961.503020795201144No Hit
TATTGCACTTGTCCCGGCCTGTAA952261.2566434136740898No Hit
TATTGCACTTGTCCCGGCCTGTAT865631.1423227250737218No Hit
AGCAGCATTGTACAGGGCTATGA641600.8466830636730474No Hit
TGAGGTAGTAGGTTGTATAGTT579280.7644429007551791No Hit
CATAAAGTAGAAAGCACTACT511960.6756045219421031No Hit
TGTAAACATCCCCGACTGGAAGCT506000.6677394485950154No Hit
TAGCTTATCAGACTGATGTTGACA418430.5521782954063484No Hit
TGAGGTAGTAGATTGTATAGTT416900.550159241342415No Hit
TTCAAGTAATTCAGGATAGGTT393820.5197018767701364No Hit
CCCATAAAGTAGAAAGCACTAC362150.477908777289891No Hit
TATTGCACTTGTCCCGGCCTGTA360860.47620643758892744No Hit
TTCTCACTACTGCACTTGACTAG357430.471680061484815No Hit
TATTGCACTTGTCCCGGCCTGT339750.4483487700793607No Hit
CCCCCACTGCTAAATTTGACTGGCTT333500.44010100021035115No Hit
TGTCAGTTTGTCAAATACCCCAA318580.42041192397905147No Hit
CAAAGAATTCTCCTTTTGGGCT313280.4134178151301313No Hit
TGTAAACATCCTACACTCTCAGCT310010.40910258193466553No Hit
CTTCTCACTACTGCACTTGACTAG309810.4088386532988572No Hit
TTAATGCTAATCGTGATAGGGGTT298440.393834310353155No Hit
ACTGGACTTGGAGTCAGAAGGC285490.37674493118456714No Hit
CAACGGAATCCCAAAAGCAGCTGT275770.36391799948428344No Hit
TGTCAGTTTGTCAAATACCCCA222800.29401650029045345No Hit
TGATGTAGTAGGTTGTATAGTT209600.27659721032710527No Hit
CAAAGAATTCTCCTTTTGGGCTT206940.27308695947085476No Hit
CCCCCCACTGCTAAATTTGACTGGCT206190.2720972270865736No Hit
AACATTCAACGCTGTCGGTGAGTT201290.2656309755092701No Hit
TGAGGTAGTAGTTTGTACAGTT186180.24569116707395255No Hit
CCCATAAAGTAGAAAGCACT173060.22837744856492762No Hit
CCCCCCACTGCTAAATTTGACTGG160610.21194789098586056No Hit
TAGCAGCACGTAAATATTGGC159010.2098364618993941No Hit
TTCAAGTAATCCAGGATAGGCTT157390.2076986399493468No Hit
CATTGCACTTGTCTCGGTCTGA153730.2028687459140548No Hit
TGATGTAGTAGATTGTATAGTT151390.19978078087509762No Hit
CTCCCACTGCTTCACTTGACTAG149410.1971678873805954No Hit
CTCCCACTGCTTCACTTGACTAGCCT137150.1809890620055462No Hit
CGCCTGTCTGAGCGTCGCT134950.17808584701165484No Hit
ACCCCCCACTGCTAAATTTGACTG131980.17416650676990147No Hit
AGCAGCATTGTACAGGGCTATGT130300.1719495062291117No Hit
TGTAAACATCCCCGACTGGAAGC130090.17167238116151298No Hit
TTTAAACATCCTTGACTGGAAGCT128480.1695477556432561No Hit
ATCACATTGCCAGGGATTTCCA127030.16763427303364586No Hit
TGTAAACATCCTACACTCAGCT125160.16516654028883823No Hit
CAACACCAGTCGATGGGCTGT124660.16450671869931743No Hit
TTATAAAGCAATGAGACTGATT120740.15933371743747465No Hit
TATTGCACTTGTCCCGGCCTGTAG118600.15650968103432578No Hit
ATCACATTGCCAGGGATTTCC114910.15164019770366252No Hit
ACTTGACTTGGAGTCAGAAGGC114660.15131028690890214No Hit
TGCAAGTAATCCAGGATAGGCT112210.14807716112025038No Hit
CGCCCCACTGCTAAATTTGACTG110470.1457809819887181No Hit
CCCCCCACTGCTAAATTTGACTGTA109420.1443953566507245No Hit
TTCACAGTGGCTAAGTTCCG109060.14392028510626956No Hit
CAACGGAATCCCAAAAGCAGCT105980.13985578411482164No Hit
ACTGGACTTGGAGTCAGAAGGCA105870.13971062336512707No Hit
CCCGCCACTGCTAAATTTGACTG102040.134656389989398No Hit
CTCCCCACTGCTAAATTTGACTG101420.13383821121839223No Hit
TAGCAGCACGTAAATATTGGCGT100590.13274290737978778No Hit
TAGCTTATCAGACTGATGTTGACC99050.1307106568840638No Hit
TGAGAACTGAATTCCATAGGCTGT97840.12911388863742357No Hit
CACTAGATTGTGAGCTCCTGGAA96610.12749072752720247No Hit
GGAATACCGGGTGCTGTAGGCTTT96160.12689688809663377No Hit
TGTCAGTTTGTCAAATACCCC92660.1222781369699884No Hit
CAGTGCAATAGTATTGTCAAAGC92510.12208019049313218No Hit
TAGCAGCACATCATGGTTTACA91120.12024588647426444No Hit
ATGCACCTGGGCAAGGATTCTGA90930.11999515427024655No Hit
TATTGCACTTGTCCCGGCCTGTAAT89950.11870190395478586No Hit
CTACGCCTGTCTGAGCGTCGCT89950.11870190395478586No Hit
TGAGGTAGTAGTTTGTGCTGTT89880.11860952893225296No Hit
TGTAAACATCCTTGACTGGAAGC86040.11354209912473347No Hit
TTCAAGTAATCCAGGATAGGC86040.11354209912473347No Hit
TATTGCACTTGTCCCGGCCTGTT85700.11309342044385935No Hit
CACCCGGCTGTGTGCACATGTGC82270.10856704433974688No Hit
AGCTCGGTCTGAGGCCCCTCAGT80360.10604652586777756No Hit
ACTGGACTTGGAGTCAGAAGGCT79320.10467409696157436No Hit
CACTAGATTGTGAGCTCCTGGA76850.10141457830934178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)