FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10898780
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8327567.640818513631801No Hit
CCCCCCACTGCTAAATTTGACTG7823257.178096998012622No Hit
TAGCAGCACGTAAATATTGGCG4350483.991712833913521No Hit
TTCAAGTAATCCAGGATAGGCT3508153.218846513095961No Hit
AACATTCAACGCTGTCGGTGAGT3347523.071463044487548No Hit
AAGCTGCCAGTTGAAGAACTGT3013122.7646397119677615No Hit
CCCCCACTGCTAAATTTGACTG2956942.7130926580773265No Hit
TAGCTTATCAGACTGATGTTGA2864612.6283767540954126No Hit
TAGCTTATCAGACTGATGTTGAC2751392.524493567169903No Hit
TAGCTTATCAGACTGATGTTGACT2672062.4517056037464746No Hit
AACATTCAACGCTGTCGGTGAG2516462.3089373306003056No Hit
TATTGCACTTGTCCCGGCCTGTAA1585031.4543187402626716No Hit
TATTGCACTTGTCCCGGCCTGTAT1474541.3529404208544442No Hit
TGAGGTAGTAGGTTGTATAGTT1099181.0085349002365402No Hit
TGTAAACATCCTTGACTGGAAGCT1037730.952152442750473No Hit
CATAAAGTAGAAAGCACTACT1034520.9492071589664164No Hit
TTCAAGTAATTCAGGATAGGTT944260.8663905501349692No Hit
TATTGCACTTGTCCCGGCCTGT906280.8315426130264122No Hit
TGAGGTAGTAGATTGTATAGTT771190.7075929599459756No Hit
AGCAGCATTGTACAGGGCTATGA733790.6732771924931047No Hit
TTCTCACTACTGCACTTGACTAG717740.6585507735728219No Hit
TATTGCACTTGTCCCGGCCTGTA701740.6438702313469948No Hit
TGTAAACATCCCCGACTGGAAGCT599530.550089092540633No Hit
CTTCTCACTACTGCACTTGACTAG559330.5132042301982424No Hit
TCGTACGACTCTTAGCGG508190.46628154710894243No Hit
CATTGCACTTGTCTCGGTCTGA410830.37695044766478447No Hit
TGATGTAGTAGGTTGTATAGTT404260.37092225001330426No Hit
CCCATAAAGTAGAAAGCACT402680.3694725464685038No Hit
CAACGGAATCCCAAAAGCAGCTGT399950.36696767895122206No Hit
CTCCCACTGCTTCACTTGACTAG387380.35543427796505667No Hit
TAGCTTATCAGACTGATGTTGACA358270.32872486645294247No Hit
CAACACCAGTCGATGGGCTGT352940.3238344108239638No Hit
ACTGGACTTGGAGTCAGAAGGC347980.31928344273395737No Hit
CCCCCACTGCTAAATTTGACTGGCTT330640.30337340509671723No Hit
TGAGGTAGTAGTTTGTACAGTT309810.28426117418646857No Hit
TAGCAGCACGTAAATATTGGC308630.28317848419731384No Hit
CAAAGAATTCTCCTTTTGGGCT306260.28100392888011316No Hit
TGATGTAGTAGATTGTATAGTT291380.26735102461009397No Hit
CCCATAAAGTAGAAAGCACTAC287960.26421305870932343No Hit
TGTAAACATCCTACACTCTCAGCT280990.2578178475021975No Hit
CACCCGGCTGTGTGCACATGTGC254160.23320041325726365No Hit
CCCCCCACTGCTAAATTTGACTGG237900.21828131222026687No Hit
CAAAGAATTCTCCTTTTGGGCTT226880.20817008876222845No Hit
AACATTCAACGCTGTCGGTGAGTT223450.20502294752256675No Hit
TTCAAGTAATCCAGGATAGGCTT213980.19633390159265532No Hit
CCCCCCACTGCTAAATTTGACTGGCT207880.19073694486905873No Hit
TGTAAACATCCCCGACTGGAAGC207180.1900946711466788No Hit
CAACGGAATCCCAAAAGCAGCT206930.18986528767440025No Hit
AGAATTACTCTGAGACCT204340.1874888749015945No Hit
CGCCTGTCTGAGCGTCGCT203280.18651628897913344No Hit
TGTCAGTTTGTCAAATACCCCA198720.18233233444477273No Hit
TGTCAGTTTGTCAAATACCCCAA189610.17397360071494242No Hit
TATTGCACTTGTCCCGGCCTGTT185730.17041356922517933No Hit
TATTGCACTTGTCCCGGCCTGTAG183910.1687436575469915No Hit
TTCACAGTGGCTAAGTTCCG177660.16300907074002777No Hit
ACCCCCCACTGCTAAATTTGACTG177460.16282556396220493No Hit
TGAGGTAGTAGTTTGTGCTGTT167560.15374197845997442No Hit
GGAATACCGGGTGCTGTAGGCTTT167250.15345754295434902No Hit
AGCTCGGTCTGAGGCCCCTCAGT166960.1531914581265059No Hit
TTATAAAGCAATGAGACTGATT165760.15209041745956886No Hit
TTCAAGTAATCCAGGATAGGC163080.1496314266367428No Hit
AACGGAATCCCAAAAGCAGCTG160480.1472458385250459No Hit
AGCAGCATTGTACAGGGCTATGT159040.14592458972472147No Hit
CTCCCACTGCTTCACTTGACTAGCCT152470.13989639207324123No Hit
TAGCTTATCAGACTGATGTTG148940.13665749744466812No Hit
ACTTGACTTGGAGTCAGAAGGC142740.1309687873321601No Hit
CACTAGATTGTGAGCTCCTGGA139840.12830793905372895No Hit
TGCAAGTAATCCAGGATAGGCT135830.12462862815838104No Hit
CACTAGATTGTGAGCTCCTGGAA135360.12419738723049736No Hit
GGAATACCGGGTGCTGTAGGCTT135020.12388542570819855No Hit
CTACGCCTGTCTGAGCGTCGCT130480.11971982185162008No Hit
TTCACAGTGGCTAAGTTCTG129690.11899497007921989No Hit
AGCAGCATTGTACAGGGCTATG126770.11631577112300644No Hit
AACATTCAACGCTGTCGGTGA122710.1125905835332028No Hit
TATTGCACTTGTCCCGGCCTGTTT122010.11194830981082286No Hit
TAGCAGCACGTAAATATTGGCGT121550.11152624422183033No Hit
TATTGCACTTGTCCCGGCCTGTAAT119660.10979210517140452No Hit
TTCACAGTGGCTAAGTTCCGC117980.10825064823769266No Hit
TTCACAGTGGCTAAGTTCTGC117200.10753497180418359No Hit
TGATGTAGTAGTTTGTACAGTT116960.10731476367079618No Hit
TGTAAACATCCTACACTCAGCT116320.10672754198176308No Hit
TCGTTTCCCGGCCAATGCACCA113880.10448875929232446No Hit
ACTGGACTTGGAGTCAGAAGGCT112400.10313080913643545No Hit
CAAAGTGCTGTTCGTGCAGGT112020.10278214625857206No Hit
ATCACATTGCCAGGGATTTCC111310.10213069719730099No Hit
CCCATAAAGTAGAAAGCACTA109440.10041490882465742No Hit
TATTGCACTTGTCCCGGCCTGTAAA109360.1003415061135283No Hit
ATGCACCTGGGCAAGGATTCTGA109000.10001119391344719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)