FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7295460
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7145719.794735355961103No Hit
AAGCTGCCAGTTGAAGAACTGT4229465.797386319711163No Hit
TAGCAGCACGTAAATATTGGCG3831385.251731899016648No Hit
TTCAAGTAATCCAGGATAGGCT3811515.224495782308449No Hit
CCCCCCACTGCTAAATTTGACTG3440674.716179651454466No Hit
TAGCTTATCAGACTGATGTTGAC2407593.300120897105871No Hit
TAGCTTATCAGACTGATGTTGACT1826832.504064171416196No Hit
AACATTCAACGCTGTCGGTGAGT1706532.339167098442045No Hit
TAGCTTATCAGACTGATGTTGA1389191.9041842460927754No Hit
TGTAAACATCCTTGACTGGAAGCT1235641.6937108832068164No Hit
TGAGGTAGTAGGTTGTATAGTT1193741.6362779043405076No Hit
GCCTGTCTGAGCGTCGCT1091861.4966294106197553No Hit
CATAAAGTAGAAAGCACTACT976621.3386681580051156No Hit
AACATTCAACGCTGTCGGTGAG969061.3283055489304307No Hit
CCCCCACTGCTAAATTTGACTG932361.278000290591683No Hit
TATTGCACTTGTCCCGGCCTGTAA843471.1561573910349725No Hit
TATTGCACTTGTCCCGGCCTGTAT741821.016824161876016No Hit
TGAGGTAGTAGATTGTATAGTT737671.011135692608828No Hit
AGCAGCATTGTACAGGGCTATGA722800.9907531533309757No Hit
TTCAAGTAATTCAGGATAGGTT601520.8245127791804765No Hit
TATTGCACTTGTCCCGGCCTGT555720.7617340099184973No Hit
TGTAAACATCCTACACTCTCAGCT493680.6766948211627506No Hit
TGTAAACATCCCCGACTGGAAGCT492380.6749128910308603No Hit
CAACGGAATCCCAAAAGCAGCTGT464990.6373689938674189No Hit
ACTGGACTTGGAGTCAGAAGGC427020.5853229268613631No Hit
CCCATAAAGTAGAAAGCACTAC383880.5261902607923283No Hit
TATTGCACTTGTCCCGGCCTGTA360190.49371801092734385No Hit
TGAGGTAGTAGTTTGTACAGTT339310.4650974715782144No Hit
CATTGCACTTGTCTCGGTCTGA318000.43588752457007507No Hit
TAGCTTATCAGACTGATGTTGACA305200.41834236634838656No Hit
CGCCTGTCTGAGCGTCGCT252210.345708152741568No Hit
TTCTCACTACTGCACTTGACTAG243640.3339611210259531No Hit
TGTAAACATCCTACACTCAGCT243630.33394741387109245No Hit
CTTCTCACTACTGCACTTGACTAG243070.33317981319889356No Hit
CCCATAAAGTAGAAAGCACT239320.32803963012613324No Hit
CAAAGAATTCTCCTTTTGGGCT229500.31457920405293155No Hit
TAGCAGCACGTAAATATTGGC211910.29046831865297046No Hit
CAACACCAGTCGATGGGCTGT200080.27425275445276925No Hit
TGAGGTAGTAGTTTGTGCTGTT196640.2695374931806905No Hit
TTCAAGTAATCCAGGATAGGCTT193490.2652197393995718No Hit
AACGGAATCCCAAAAGCAGCTG176040.24130075416766042No Hit
CAAAGAATTCTCCTTTTGGGCTT169860.23282973246375144No Hit
CCCCCCACTGCTAAATTTGACTGGCT167180.22915621496108537No Hit
CAACGGAATCCCAAAAGCAGCT166530.22826524989514027No Hit
CCCCCACTGCTAAATTTGACTGGCTT165830.22730574905489168No Hit
CTCCCACTGCTTCACTTGACTAG163730.2244272465341459No Hit
TGTCAGTTTGTCAAATACCCCA158810.21768332634268436No Hit
TAGCAGCACGTAAATATTGGCGT156060.21391385875599345No Hit
TTCACAGTGGCTAAGTTCCGC153210.2100073196206956No Hit
TGTAAACATCCCCGACTGGAAGC153100.20985654091722794No Hit
TTCACAGTGGCTAAGTTCCG152580.20914376886447186No Hit
TTCAAGTAATCCAGGATAGGC149830.20537430127778097No Hit
AGCTCGGTCTGAGGCCCCTCAGT149630.20510015818056712No Hit
AACATTCAACGCTGTCGGTGAGTT147670.20241355582787104No Hit
TTATAAAGCAATGAGACTGATT136420.1869930066095901No Hit
CCCCCCACTGCTAAATTTGACTGG132220.18123600156809852No Hit
TGTCAGTTTGTCAAATACCCCAA130610.17902914963552674No Hit
TTCACAGTGGCTAAGTTCTGC126440.1733132660586173No Hit
AGCAGCATTGTACAGGGCTATGT121250.16619925268591698No Hit
ATCACATTGCCAGGGATTTCCA116920.1602640546312364No Hit
ATCACATTGCCAGGGATTTCC112410.15408212778906333No Hit
TACGCCTGTCTGAGCGTCGCT111680.15308150548423266No Hit
TATTGCACTTGTCCCGGCCTGTT108050.1481058082698007No Hit
TGTAAACATCCTTGACTGGAAGC106300.14570705616917917No Hit
ACTGGACTTGGAGTCAGAAGGCA106130.1454740345365474No Hit
TGTCAGTTTGTCAAATACCCC106110.145446620226826No Hit
CTACGCCTGTCTGAGCGTCGCT105210.14421297628936353No Hit
AGCAGCATTGTACAGGGCTATG104830.14369210440465713No Hit
TGAGGTAGTAGGTTGTATAGT100460.13770207773053378No Hit
AATGACACGATCACTCCCGTTG96910.13283603775498734No Hit
TGAGGTAGTAGGTTGTGTGGTT96830.13272638051610178No Hit
TAGCAGCACATAATGGTTTGT96640.1324659445737486No Hit
AGAGGTAGTAGGTTGCATAGTT94750.1298752923050774No Hit
CAAAGTGCTGTTCGTGCAGGTAG93690.12842233388984384No Hit
CACCCGGCTGTGTGCACATGTGC93370.1279837049343016No Hit
TAGCAGCACATCATGGTTTACA92340.1265718679836501No Hit
AACCGGGCGGAAACACCA88270.12099305595534757No Hit
CACTAGATTGTGAGCTCCTGGA88000.12062296277410883No Hit
ACCACAGGGTAGAACCACGGAC86360.118374989376955No Hit
ACTGGACTTGGAGTCAGAAGG85690.11745661000128846No Hit
CTCCCACTGCTTCACTTGACTAGCCT85640.117388074226985No Hit
ACCCCCCACTGCTAAATTTGACTG84610.1159762372763335No Hit
GTAAACATCCTTGACTGGAAGCT83750.11479742195831381No Hit
ACTGGACTTGGAGTCAGAAGGCT83490.11444103593193575No Hit
CAGTGCAATAGTATTGTCAAAGC83350.11424913576388604No Hit
TATTGCACTTGTCCCGGCCTGTAG82950.11370084956945828No Hit
TAGCTTATCAGACTGATGTTGACC80920.11091829713273733No Hit
CACTAGATTGTGAGCTCCTGGAA80840.1108086398938518No Hit
CAAAGTGCTGTTCGTGCAGGT80670.11057561826121999No Hit
TTCAAGTAATCCAGGATAGGCA79500.10897188114251877No Hit
GCGTTCGCGCTTTCCCCTG77490.10621674301551924No Hit
CCCATAAAGTAGAAAGCACTA76090.10429774133502207No Hit
ATGCACCTGGGCAAGGATTCTGA74730.10243356827396766No Hit
TATTGCACTTGTCCCGGCCTGTAAT74020.10146036027885835No Hit
TCGTTTCCCGGCCAATGCACCA73810.10117251002678379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)