FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9779927
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG99864010.211119162750396No Hit
CCCCCCACTGCTAAATTTGACTG5807235.937907307488083No Hit
TAGCAGCACGTAAATATTGGCG3001863.069409413792148No Hit
TTCAAGTAATCCAGGATAGGCT2934013.0000326178303784No Hit
AAGCTGCCAGTTGAAGAACTGT2858622.922946152870057No Hit
AACATTCAACGCTGTCGGTGAGT2728602.790000375258425No Hit
TAGCTTATCAGACTGATGTTGACT2627072.6861856944330973No Hit
TATTGCACTTGTCCCGGCCTGTAT2545852.6031380397829142No Hit
TATTGCACTTGTCCCGGCCTGTAA2336012.3885761110486814No Hit
TAGCTTATCAGACTGATGTTGAC2331412.383872599458053No Hit
AACATTCAACGCTGTCGGTGAG2307772.359700639892302No Hit
GCCTGTCTGAGCGTCGCT2211702.2614688228245465No Hit
CCCCCACTGCTAAATTTGACTG1704251.7425999192018509No Hit
TAGCTTATCAGACTGATGTTGA1637441.6742865258605713No Hit
TATTGCACTTGTCCCGGCCTGT1236671.2644981910396673No Hit
TATTGCACTTGTCCCGGCCTGTA1115931.141041236810868No Hit
TGAGGTAGTAGGTTGTATAGTT1087221.1116851894702282No Hit
TGTAAACATCCTTGACTGGAAGCT918150.9388106884642391No Hit
CATAAAGTAGAAAGCACTACT819230.8376647392153337No Hit
AGCAGCATTGTACAGGGCTATGA764720.7819281268663867No Hit
TCCTGTCTGAGCGTCGCT731980.7484513943713486No Hit
TGAGGTAGTAGATTGTATAGTT726100.7424390795555018No Hit
TTCAAGTAATTCAGGATAGGTT722260.7385126698798468No Hit
TGTAAACATCCCCGACTGGAAGCT630480.6446673886216124No Hit
CATTGCACTTGTCTCGGTCTGA488340.4993288804711937No Hit
CAACGGAATCCCAAAAGCAGCTGT460840.4712100611793933No Hit
ACTGGACTTGGAGTCAGAAGGC436540.4463632499506387No Hit
CCCATAAAGTAGAAAGCACT405960.41509512289815664No Hit
TTAATGCTAATCGTGATAGGGGTT392030.4008516627987101No Hit
CCCATAAAGTAGAAAGCACTAC359430.36751808065643027No Hit
CAACACCAGTCGATGGGCTGT344050.35179199190341603No Hit
TAGCTTATCAGACTGATGTTGACA339450.3470884803127876No Hit
CACTAGATTGTGAGCTCCTGGA328540.3359329778228406No Hit
TGTAAACATCCTACACTCTCAGCT322560.32981841275502366No Hit
CACTAGATTGTGAGCTCCTGGAA321190.32841758430303214No Hit
TATTGCACTTGTCCCGGCCTGTAG310550.31753815749340464No Hit
ATCCCGGACGAGCCCCCA296080.30274254603331907No Hit
TGATGTAGTAGGTTGTATAGTT283100.28947046332758924No Hit
TATTGCACTTGTCCCGGCCTGTT272680.27881598707229616No Hit
CAAAGAATTCTCCTTTTGGGCT267530.27355009909583167No Hit
CACCCGGCTGTGTGCACATGTGC266960.2729672726596017No Hit
TGAGAACTGAATTCCATAGGCTGT254820.26055409207042135No Hit
CAACGGAATCCCAAAAGCAGCT253790.25950091447512846No Hit
TGAGGTAGTAGTTTGTACAGTT250260.25589148058058103No Hit
CTCCCACTGCTTCACTTGACTAG238700.24407135145282782No Hit
TATTGCACTTGTCCCGGCCTGTTT216100.22096279450756637No Hit
TCGTACGACTCTTAGCGG214550.21937791560202852No Hit
TAGCAGCACGTAAATATTGGC202820.207383961045926No Hit
TATTGCACTTGTCCCGGCCTGTAAT202260.20681135963489297No Hit
AACGGAATCCCAAAAGCAGCTG200300.2048072546962774No Hit
TGATGTAGTAGATTGTATAGTT197090.2015250216080345No Hit
AACATTCAACGCTGTCGGTGAGTT183080.1871997613070118No Hit
CAAAGAATTCTCCTTTTGGGCTT179710.18375392781561664No Hit
TTCTCACTACTGCACTTGACTAG178290.18230197423764No Hit
TATTGCACTTGTCCCGGCCTGTAAA177580.18157599744865172No Hit
CTTCTCACTACTGCACTTGACTAG174490.17841646466277306No Hit
TTCAAGTAATCCAGGATAGGCTT174340.17826308928481777No Hit
TTATAAAGCAATGAGACTGATT173800.17771093792417877No Hit
CCCCCACTGCTAAATTTGACTGGCTT167970.1717497482343171No Hit
AGCTCGGTCTGAGGCCCCTCAGT167140.17090107114296457No Hit
AGCAGCATTGTACAGGGCTATGT161890.16553293291452995No Hit
TGAGGTAGTAGTTTGTGCTGTT161870.1655124828641359No Hit
TGTAAACATCCCCGACTGGAAGC158620.16218934967510493No Hit
ACTGGACTTGGAGTCAGAAGGCA153550.15700526190021663No Hit
TGAGAACTGAATTCCATGGGTT139720.14286405205274028No Hit
TCGTTTCCCGGGCGGCGCACCA139120.14225055054091917No Hit
TTCACAGTGGCTAAGTTCCGC136550.13962271906528545No Hit
ACTTGACTTGGAGTCAGAAGGC133940.13695398748886367No Hit
ACTGGACTTGGAGTCAGAAGGCT132280.1352566333061586No Hit
TATTGCACTTGTCCCGGCCTGA122020.12476575745401781No Hit
TTCAAGTAATCCAGGATAGGC119710.12240377663350656No Hit
TTCACAGTGGCTAAGTTCTG119380.12206635080200497No Hit
TTCACAGTGGCTAAGTTCCG117900.12055304707284625No Hit
TGTCAGTTTGTCAAATACCCCA116470.11909086846967262No Hit
ACCCCCCACTGCTAAATTTGACTG114620.11719923880822423No Hit
ATGCACCTGGGCAAGGATTCTGA113970.11653461217041804No Hit
CCCATAAAGTAGAAAGCACTA113690.11624831146490153No Hit
CCCCCCACTGCTAAATTTGACTGGCT113450.11600291086017309No Hit
AACCGGGCGGAAACACCA109710.11217875143648823No Hit
ACCACAGGGTAGAACCACGGAC108230.1106654477073295No Hit
CCCCCCACTGCTAAATTTGACTGG107410.10982699564117401No Hit
TCGTTTCCCGGCCAATGCACCA106240.10863066769312288No Hit
AGCAGCATTGTACAGGGCTATG105310.10767974034980016No Hit
TTCACAGTGGCTAAGTTCTGC102940.10525640937810682No Hit
CGCCTGTCTGAGCGTCGCT102910.10522573430251575No Hit
TAGCTTATCAGACTGATGTTGACC102700.10501100877337838No Hit
TGTCAGTTTGTCAAATACCCCAA102610.10491898354660521No Hit
TATGGCACTTGTCCCGGCCTGTAT101510.10379423077493319No Hit
CAAAGTGCTGTTCGTGCAGGTAG99540.10177990081112057No Hit
TGTAAACATCCTACACTCAGCT98480.10069604814023662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)