FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13919816
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9454536.792137194916944No Hit
AAGCTGCCAGTTGAAGAACTGT7176975.155937406069161No Hit
TAGCAGCACGTAAATATTGGCG5673354.0757363459402045No Hit
AACATTCAACGCTGTCGGTGAGT5516793.96326359486361No Hit
TTCAAGTAATCCAGGATAGGCT4735813.4022073280278993No Hit
TAGCTTATCAGACTGATGTTGAC4542693.263469861957945No Hit
CCCCCCACTGCTAAATTTGACTG4467203.209237823258583No Hit
TAGCTTATCAGACTGATGTTGACT3873522.782737932742789No Hit
TAGCTTATCAGACTGATGTTGA3053822.1938652062642205No Hit
AACATTCAACGCTGTCGGTGAG2693421.9349537378942367No Hit
TATTGCACTTGTCCCGGCCTGTAA2315641.6635564722981973No Hit
TATTGCACTTGTCCCGGCCTGTAT1928811.38565768398088No Hit
TGTAAACATCCTTGACTGGAAGCT1848981.3283077879765077No Hit
CATAAAGTAGAAAGCACTACT1790371.286202346352854No Hit
GCCTGTCTGAGCGTCGCT1584341.1381903324009455No Hit
CCCCCACTGCTAAATTTGACTG1485861.0674422707886368No Hit
TGAGGTAGTAGGTTGTATAGTT1418201.018835306443706No Hit
TATTGCACTTGTCCCGGCCTGT1345910.9669021487065634No Hit
AGCAGCATTGTACAGGGCTATGA1232100.8851410104846213No Hit
TTCAAGTAATTCAGGATAGGTT1009560.7252682075682609No Hit
TGAGGTAGTAGATTGTATAGTT978430.7029044062076683No Hit
TATTGCACTTGTCCCGGCCTGTA946480.6799515166005068No Hit
TGTAAACATCCCCGACTGGAAGCT936040.6724514174612654No Hit
TCCTGTCTGAGCGTCGCT889210.6388087313797826No Hit
TTAATGCTAATCGTGATAGGGGTT876710.6298287276211122No Hit
CGCCTGTCTGAGCGTCGCT809710.5816959074746391No Hit
TGTAAACATCCTACACTCTCAGCT685730.49262863819464275No Hit
ACTGGACTTGGAGTCAGAAGGC673260.4836701864449932No Hit
CAACGGAATCCCAAAAGCAGCTGT636760.45744857546967577No Hit
CCCATAAAGTAGAAAGCACTAC618840.44457484208124587No Hit
TCGTACGACTCTTAGCGG527600.3790279986459591No Hit
TAGCTTATCAGACTGATGTTGACA523180.37585266931689326No Hit
TGATGTAGTAGGTTGTATAGTT515760.3705221390857465No Hit
CATTGCACTTGTCTCGGTCTGA508810.3655292569959258No Hit
CCCATAAAGTAGAAAGCACT491440.3530506437728775No Hit
AACATTCAACGCTGTCGGTGAGTT486160.34925749018521507No Hit
CAACACCAGTCGATGGGCTGT454830.3267500087644837No Hit
TGAGGTAGTAGTTTGTACAGTT428090.307539984723936No Hit
TGATGTAGTAGATTGTATAGTT360000.2586241082497067No Hit
TTATAAAGCAATGAGACTGATT349860.2513395292006733No Hit
CAACGGAATCCCAAAAGCAGCT349370.25098751305333344No Hit
CAAAGAATTCTCCTTTTGGGCT347130.2493782963797797No Hit
TTCAAGTAATCCAGGATAGGCTT334680.24043421263614403No Hit
CAAAGAATTCTCCTTTTGGGCTT321100.23067833655272454No Hit
TGTAAACATCCTACACTCAGCT291200.209198167561985No Hit
TAGCAGCACGTAAATATTGGC276460.1986089471297609No Hit
ACTTGACTTGGAGTCAGAAGGC274270.19703565047124186No Hit
TACGCCTGTCTGAGCGTCGCT267640.1922726564776431No Hit
AGCTCGGTCTGAGGCCCCTCAGT265280.19057723176800614No Hit
CTACGCCTGTCTGAGCGTCGCT264930.19032579166276337No Hit
TTCACAGTGGCTAAGTTCCG256390.18419065309483976No Hit
TGAGGTAGTAGTTTGTGCTGTT254000.18247367637618198No Hit
TTCTCACTACTGCACTTGACTAG251960.18100813976276697No Hit
TGTAAACATCCCCGACTGGAAGC250020.17961444317942135No Hit
TATTGCACTTGTCCCGGCCTGTT248830.17875954682159592No Hit
AGCAGCATTGTACAGGGCTATGT245780.17656842590448035No Hit
TGCAAGTAATCCAGGATAGGCT244320.17551956146546766No Hit
TATTGCACTTGTCCCGGCCTGTAG244270.17548364145043296No Hit
CACCCGGCTGTGTGCACATGTGC231090.16601512548729092No Hit
TTTAAACATCCTTGACTGGAAGCT230790.16579960539708283No Hit
ACGCCTGTCTGAGCGTCGCT221910.1594202107269234No Hit
TAGCAGCACGTAAATATTGGCGT219660.15780381005036273No Hit
AACCGGGCGGAAACACCA219360.15758828996015464No Hit
ACCACAGGGTAGAACCACGGAC219100.1574015058819743No Hit
CTTCTCACTACTGCACTTGACTAG218130.15670465759030147No Hit
TCGTTTCCCGGCCAATGCACCA205620.14771746982862416No Hit
CACTAGATTGTGAGCTCCTGGA195300.14030357872546592No Hit
TTCACAGTGGCTAAGTTCCGC192360.13819148184142663No Hit
TTCAAGTAATCCAGGATAGGC192060.13797596175121854No Hit
ACTGGACTTGGAGTCAGAAGGCA184840.13278911158021053No Hit
CACTAGATTGTGAGCTCCTGGAA184810.13276755957118974No Hit
TGTCAGTTTGTCAAATACCCCA184730.13271008754713426No Hit
AGCAGCATTGTACAGGGCTATG180010.1293192381278603No Hit
TATTGCACTTGTCCCGGCCTGTAAT176410.12673299704536323No Hit
AACGGAATCCCAAAAGCAGCTG175970.12641690091305804No Hit
TTCACAGTGGCTAAGTTCTGC174250.125181252395865No Hit
CAAAGTGCTGTTCGTGCAGGT173380.12455624413426154No Hit
CAGTGCAATAGTATTGTCAAAGC173170.12440538007111587No Hit
CAAAGTGCTGTTCGTGCAGGTAG165470.11887369775577493No Hit
GCGTTCGCGCTTTCCCCTG160950.11562652839663973No Hit
TGTAAACATCCTTGACTGGAAGC159050.11426156782532183No Hit
TGATGTAGTAGTTTGTACAGTT158410.1138017916328779No Hit
TGTCAGTTTGTCAAATACCCCAA154540.11102158246919355No Hit
TTCACAGTGGCTAAGTTCTG154280.11083479839101322No Hit
ATCCCGGACGAGCCCCCA153700.1104181262166109No Hit
TGAGAACTGAATTCCATAGGCTGT151290.10868678149193926No Hit
TATTGCACTTGTCCCGGCCTGTAAA151150.10858620544984215No Hit
TAGCTTATCAGACTGATGTTGACC150410.10805458922732887No Hit
TATTGCACTTGTCCCGGCCTGTTT150390.10804022122131499No Hit
CCCCCACTGCTAAATTTGACTGGCTT149760.107587629031878No Hit
CCAAGTCCTTCTGATCGAGGCCC146910.10554018817490116No Hit
CTCCCACTGCTTCACTTGACTAG145860.10478586785917285No Hit
AACATTCAACGCTGTCGGTGA143930.10339935527883415No Hit
ATCACATTGCCAGGGATTTCCA143690.10322693920666769No Hit
ACTGGACTTGGAGTCAGAAGG143260.1029180270773694No Hit
ACTGGACTTGGAGTCAGAAGGCT141610.10173266658122493No Hit
TAGCAGCACATAATGGTTTGT140020.10059041010312204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)