FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13254856
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG150007011.317135395510897No Hit
CCCCCCACTGCTAAATTTGACTG8814486.6500005733747685No Hit
GCCTGTCTGAGCGTCGCT5813424.385879409025644No Hit
TTCAAGTAATCCAGGATAGGCT5477874.132726904011632No Hit
TAGCAGCACGTAAATATTGGCG4600883.471090142359902No Hit
AAGCTGCCAGTTGAAGAACTGT4091063.0864612938835396No Hit
AACATTCAACGCTGTCGGTGAGT3677122.7741681991867733No Hit
TGAGGTAGTAGGTTGTATAGTT2616031.9736389440971673No Hit
CCCCCACTGCTAAATTTGACTG2592711.9560453919680456No Hit
AACATTCAACGCTGTCGGTGAG2559931.9313148328431482No Hit
TATTGCACTTGTCCCGGCCTGTAA2032131.5331211444318973No Hit
TATTGCACTTGTCCCGGCCTGTAT1774161.3384981323071332No Hit
TAGCTTATCAGACTGATGTTGACT1753411.3228434922265468No Hit
TAGCTTATCAGACTGATGTTGAC1591661.2008127436465548No Hit
TCGTACGACTCTTAGCGG1520921.1474436236802574No Hit
TATTGCACTTGTCCCGGCCTGT1503941.1346332242311798No Hit
CATAAAGTAGAAAGCACTACT1411071.0645683363138763No Hit
TAGCTTATCAGACTGATGTTGA1329261.0028475601696465No Hit
TGAGGTAGTAGATTGTATAGTT1182030.8917712874436358No Hit
TGTAAACATCCTTGACTGGAAGCT1057380.7977302808872461No Hit
CAACGGAATCCCAAAAGCAGCTGT1025000.7733014979566734No Hit
TTCAAGTAATTCAGGATAGGTT861860.6502220771014035No Hit
CCCATAAAGTAGAAAGCACTAC823380.6211912072073812No Hit
TATTGCACTTGTCCCGGCCTGTA818870.6177886806163718No Hit
AGCAGCATTGTACAGGGCTATGA749060.5651211903018788No Hit
ACTGGACTTGGAGTCAGAAGGC708500.5345210841973689No Hit
TGTAAACATCCCCGACTGGAAGCT608350.4589638695433583No Hit
CACCCGGCTGTGTGCACATGTGC576560.43498020649941427No Hit
CATTGCACTTGTCTCGGTCTGA536020.4043951892046206No Hit
CCCATAAAGTAGAAAGCACT532800.4019658908403079No Hit
CGCCTGTCTGAGCGTCGCT521950.3937802115692543No Hit
TGAGGTAGTAGTTTGTACAGTT521480.393425624540923No Hit
TGATGTAGTAGGTTGTATAGTT467330.3525726722342363No Hit
CAACACCAGTCGATGGGCTGT444800.3355751280889057No Hit
CAACGGAATCCCAAAAGCAGCT428140.32300614959528795No Hit
TGTAAACATCCTACACTCTCAGCT354960.2677961948436105No Hit
TTCTCACTACTGCACTTGACTAG322360.24320143500615926No Hit
TCCTGTCTGAGCGTCGCT318440.2402440283017786No Hit
AGCTCGGTCTGAGGCCCCTCAGT315530.23804860648806747No Hit
AACGGAATCCCAAAAGCAGCTG313380.23642655944357296No Hit
TAGCAGCACGTAAATATTGGC310740.2344348365610309No Hit
CTCCCACTGCTTCACTTGACTAG310320.23411797155699013No Hit
AACCGGGCGGAAACACCA289900.2187122968367216No Hit
CCCCCACTGCTAAATTTGACTGGCTT284750.21482692833479292No Hit
TTCACAGTGGCTAAGTTCCGC283480.21386878891781247No Hit
TGAGGTAGTAGTTTGTGCTGTT279840.21112262554945901No Hit
CTTCTCACTACTGCACTTGACTAG266590.2011262891124581No Hit
TGAGGTAGTAGGTTGTGTGGTT259520.1957923948777716No Hit
TCGTTTCCCGGCCAATGCACCA257010.19389874925838502No Hit
TATTGCACTTGTCCCGGCCTGTT255450.19272182210051922No Hit
TTCAAGTAATCCAGGATAGGCTT251060.18940982836780723No Hit
AACATTCAACGCTGTCGGTGAGTT237400.17910417133162368No Hit
CAAAGAATTCTCCTTTTGGGCT234610.17699928237620988No Hit
CCCCCCACTGCTAAATTTGACTGG229850.17340814566374768No Hit
TTCAAGTAATCCAGGATAGGC229070.17281968208481482No Hit
CACTAGATTGTGAGCTCCTGGA227000.17125799027918523No Hit
TGATGTAGTAGATTGTATAGTT211110.15926993095964226No Hit
CCCCCCACTGCTAAATTTGACTGGCT210750.15899833238475017No Hit
TTCACAGTGGCTAAGTTCTGC207300.1563955127087009No Hit
ATCCCGGACGAGCCCCCA204870.15456222232817918No Hit
TATTGCACTTGTCCCGGCCTGTAG204220.1540718360124018No Hit
TGAGGTAGTAGGTTGTATAGT201930.15234416729989372No Hit
CAAAGAATTCTCCTTTTGGGCTT194650.1468518405631868No Hit
TAGCTTATCAGACTGATGTTGACA190860.14399251112196165No Hit
CACTAGATTGTGAGCTCCTGGAA180650.13628967376182738No Hit
TATTGCACTTGTCCCGGCCTGTAAT178350.13455446064446117No Hit
TTATAAAGCAATGAGACTGATT175670.13253256014248666No Hit
GGAATACCGGGTGCTGTAGGCTTT174190.1314159882234858No Hit
ACCCCCCACTGCTAAATTTGACTG173360.13078980262026235No Hit
AGCAGCATTGTACAGGGCTATGT173260.1307143585716812No Hit
CTACGCCTGTCTGAGCGTCGCT170700.12878299092800405No Hit
CCCATAAAGTAGAAAGCACTA164920.1244223249200142No Hit
TGAGAACTGAATTCCATAGGCTGT162510.12260412334920877No Hit
ACTGGACTTGGAGTCAGAAGGCT161050.12150264023992415No Hit
TGTAAACATCCCCGACTGGAAGC158780.1197900603371323No Hit
TAGCAGCACGTAAATATTGGCGT154610.11664404351129881No Hit
ACCACAGGGTAGAACCACGGAC150950.11388279133322911No Hit
ATCACATTGCCAGGGATTTCC149570.11284166346280941No Hit
AGAGGTAGTAGGTTGCATAGTT148260.11185334642639648No Hit
CTCCCACTGCTTCACTTGACTAGCCT146670.11065378605395637No Hit
CAAAGTGCTGTTCGTGCAGGT145820.11001251164101669No Hit
TGAGGTAGTAAGTTGTATTGTT144510.10902419460460377No Hit
TATTGCACTTGTCCCGGCCTGTAAA144430.10896383936573886No Hit
TACGCCTGTCTGAGCGTCGCT143950.10860170793254939No Hit
TTCACAGTGGCTAAGTTCCG143490.10825466530907615No Hit
TATTGCACTTGTCCCGGCCTGTTT142060.10717581541436587No Hit
TGTAAACATCCTACACTCAGCT140990.10636856409454767No Hit
AGCAGCATTGTACAGGGCTATG133950.1010573030744355No Hit
GACCCGCGGGCGCTCTCCAGTCCT132660.10008407484773882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)