FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8039051
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG195605024.33185210542886No Hit
CCCCCCACTGCTAAATTTGACTG4137055.14619200699187No Hit
TAGCAGCACGTAAATATTGGCG2817433.5046798434292805No Hit
AACATTCAACGCTGTCGGTGAGT2393782.9776897795523376No Hit
AACATTCAACGCTGTCGGTGAG2365402.9423871051446246No Hit
TAGCTTATCAGACTGATGTTGAC1812402.254494964641971No Hit
AAGCTGCCAGTTGAAGAACTGT1805502.2459118619847045No Hit
TCGTACGACTCTTAGCGG1717522.136471083464951No Hit
TTCAAGTAATCCAGGATAGGCT1602781.9937427937700605No Hit
TGAGGTAGTAGGTTGTATAGTT1431701.780931604986708No Hit
CCCCCACTGCTAAATTTGACTG1185721.474950214894768No Hit
TAGCTTATCAGACTGATGTTGA984351.2244604493739373No Hit
TAGCTTATCAGACTGATGTTGACT736340.9159538856016711No Hit
TATTGCACTTGTCCCGGCCTGT643480.8004427388257644No Hit
TGAGAACTGAATTCCATGGGTT576550.7171866430502805No Hit
CACCCGGCTGTGTGCACATGTGC568320.7069491162576279No Hit
CATAAAGTAGAAAGCACTACT531550.6612098865898475No Hit
AGCAGCATTGTACAGGGCTATGA530400.659779369480303No Hit
CCCCCACTGCTAAATTTGACTGGCTT433360.5390686039931827No Hit
TGATGTAGTAGGTTGTATAGTT425230.5289554699926645No Hit
TATTGCACTTGTCCCGGCCTGTA419060.5212804347179786No Hit
TTAATGCTAATCGTGATAGGGGTT413430.5142771205208176No Hit
TAGCTTATCAGACTGATGTTGACA344910.4290431793503985No Hit
ACTGGACTTGGAGTCAGAAGGC343650.4274758301695063No Hit
TATTGCACTTGTCCCGGCCTGTAT336070.4180468565257267No Hit
TGTAAACATCCTTGACTGGAAGCT336010.41797222085044616No Hit
TATTGCACTTGTCCCGGCCTGTAA334810.41647950734483463No Hit
CAACGGAATCCCAAAAGCAGCTGT331260.4120635632240671No Hit
CAACGGAATCCCAAAAGCAGCT321490.3999103874325465No Hit
TTCACAGTGGCTAAGTTCCGC301400.3749198754927665No Hit
TGAGGTAGTAGATTGTATAGTT285970.3557260676664447No Hit
TAGCAGCACGTAAATATTGGC284150.35346211884960055No Hit
TTCAAGTAATTCAGGATAGGTT276450.34388387385525976No Hit
CATTGCACTTGTCTCGGTCTGA269470.33520125696428593No Hit
AACGGAATCCCAAAAGCAGCTG239570.29800781211613164No Hit
CTCCCACTGCTTCACTTGACTAGCCT222660.27697299096622224No Hit
TGTAAACATCCCCGACTGGAAGCT215220.2677181672314307No Hit
AACATTCAACGCTGTCGGTGAGTT213650.26576520039492224No Hit
TGAGAACTGAATTCCATAGGCTGT209330.2603914317747207No Hit
TGAGGTAGTAGGTTGTGTGGTT184600.22962909427990938No Hit
CACTAGATTGTGAGCTCCTGGAA176840.21997621361028807No Hit
CACTAGATTGTGAGCTCCTGGA172380.2144282950810985No Hit
TGAGGTAGTAGTTTGTGCTGTT165750.2061810529625947No Hit
TAGCAGCACGTAAATATTGGCGT165580.20596958521596642No Hit
TGATAACTGAATTCCATGGGTT164410.20451418954799513No Hit
CAAAGAATTCTCCTTTTGGGCT162990.20274781189968816No Hit
TGTAAACATCCTACACTCTCAGCT156990.19528424437163044No Hit
CCCATAAAGTAGAAAGCACTAC152050.18913924044019625No Hit
ACCCCCCACTGCTAAATTTGACTG146070.18170055147056538No Hit
AATCCTTGGAACCTAGGTGTGAGT135950.16911200090657466No Hit
TTCACAGTGGCTAAGTTCTGC135290.16829100847848832No Hit
CAACACCAGTCGATGGGCTGT130990.16294211841671363No Hit
TTCACCACCTTCTCCACCCAGC127900.15909838113976388No Hit
TATTGCACTTGTCCCGGCCTGTT121020.15054015704092436No Hit
CGACGGAATCCCAAAAGCAGCTG117880.1466342233679075No Hit
ATCACATTGCCAGGGATTTCC116300.1446688172521856No Hit
ACTTGACTTGGAGTCAGAAGGC115010.1430641502336532No Hit
CCCCCCACTGCTAAATTTGACTGG114800.1428029253701712No Hit
CCCATAAAGTAGAAAGCACT109410.13609815387413265No Hit
GTCAGGATGGCCGAGCGGTCTAAGG109380.13606083603649236No Hit
AGCAGCATTGTACAGGGCTATGT109100.13571253621851634No Hit
CAAAGAATTCTCCTTTTGGGCTT106030.13189367749999345No Hit
AGCAGCATTGTACAGGGCTATG103430.12865946490450178No Hit
CCCCCCACTGCTAAATTTGACTGGCT97150.12084759755846804No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC96440.11996440873431455No Hit
AACATTCAACGCTGTCGGTGAGA94390.11741435649556148No Hit
CAAAGAATTCTCCTTTTGGGC93720.11658092478826171No Hit
CTTCTCACTACTGCACTTGACTAG93710.11656848550904828No Hit
CAAAGTGCTGTTCGTGCAGGTAG92100.11456576155568611No Hit
CTACGGAATCCCAAAAGCAGCTG91670.11403087254950864No Hit
CCCATAAAGTAGAAAGCACTA91340.11362037633546546No Hit
TCAGTGCATCACAGAACTTTGT89550.11139374535626158No Hit
GACCCGCGGGCGCTCTCCAGTCCT89500.11133154896019444No Hit
TGAGGTAGTAGTTTGTACAGTT88680.11031152806469322No Hit
ATGCACCTGGGCAAGGATTCTGA88080.10956517131188742No Hit
AATGCACCCGGGCAAGGATTCT87850.10927906788997856No Hit
TGATGTAGTAGATTGTATAGTT86070.10706487618998811No Hit
AGCTCGGTCTGAGGCCCCTCAGT85810.10674145493043893No Hit
TGAGGTAGTAAGTTGTATTGTT83540.10391773854899042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)