FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8411002
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG102231812.154532836872468No Hit
CCCCCCACTGCTAAATTTGACTG8363849.943928202608916No Hit
TTCAAGTAATCCAGGATAGGCT2926623.479514093564595No Hit
TAGCAGCACGTAAATATTGGCG2761693.283425684597388No Hit
GCCTGTCTGAGCGTCGCT2455402.9192716872496285No Hit
AAGCTGCCAGTTGAAGAACTGT2155832.5631072255124896No Hit
TATTGCACTTGTCCCGGCCTGTAA1944422.311757861905157No Hit
AACATTCAACGCTGTCGGTGAGT1789912.12805798881037No Hit
CCCCCACTGCTAAATTTGACTG1704642.0266788665607263No Hit
TATTGCACTTGTCCCGGCCTGTAT1699592.020674825662864No Hit
AACATTCAACGCTGTCGGTGAG1439881.711900674854197No Hit
TAGCTTATCAGACTGATGTTGACT1224951.4563663164032061No Hit
TAGCTTATCAGACTGATGTTGAC982501.168112907356341No Hit
TTAATGCTAATCGTGATAGGGGTT952801.132802013362974No Hit
TGTAAACATCCTTGACTGGAAGCT894771.063809044392095No Hit
TGAGGTAGTAGGTTGTATAGTT856861.0187371254934905No Hit
CAACGGAATCCCAAAAGCAGCTGT770290.9158124085572683No Hit
TCCTGTCTGAGCGTCGCT738480.8779928954956853No Hit
TATTGCACTTGTCCCGGCCTGT605080.7193911022729516No Hit
TGAGGTAGTAGATTGTATAGTT534950.6360122135269972No Hit
TAGCTTATCAGACTGATGTTGA528700.6285814698415242No Hit
TGTAAACATCCCCGACTGGAAGCT524620.6237306803636475No Hit
TATTGCACTTGTCCCGGCCTGTA520390.6187015530373194No Hit
TTCAAGTAATTCAGGATAGGTT497520.5915109757434369No Hit
TGAGAACTGAATTCCATAGGCTGT481220.5721315962117237No Hit
AGCAGCATTGTACAGGGCTATGA442050.5255616393861278No Hit
CCCATAAAGTAGAAAGCACTAC441080.5244083879661424No Hit
CCCCCACTGCTAAATTTGACTGGCTT414570.49289014554984056No Hit
CAAAGAATTCTCCTTTTGGGCT372700.4431101074521204No Hit
CACCCGGCTGTGTGCACATGTGC351730.41817847623862175No Hit
ACTGGACTTGGAGTCAGAAGGC349040.4149802841563942No Hit
AACCGGGCGGAAACACCA327030.3888121771936328No Hit
CATAAAGTAGAAAGCACTACT311740.3706336058414919No Hit
TGTAAACATCCTACACTCTCAGCT286450.3405658445925943No Hit
TGAGAACTGAATTCCATGGGTT269970.32097245964273935No Hit
TGATGTAGTAGGTTGTATAGTT254590.3026868855815276No Hit
TTCTCACTACTGCACTTGACTAG223160.2653191617360215No Hit
TAGCAGCACGTAAATATTGGC220730.26243008859110956No Hit
CAAAGAATTCTCCTTTTGGGCTT215250.25591481252768694No Hit
TATTGCACTTGTCCCGGCCTGTAAT214440.25495178814604963No Hit
TATTGCACTTGTCCCGGCCTGTAG213190.25346563940895506No Hit
TATTGCACTTGTCCCGGCCTGTAAA210580.2503625608459016No Hit
AGCTCGGTCTGAGGCCCCTCAGT206000.24491731187318705No Hit
ACCCCCCACTGCTAAATTTGACTG204310.2429080387806352No Hit
CAACGGAATCCCAAAAGCAGCT203920.24244436037466166No Hit
CACTAGATTGTGAGCTCCTGGAA202280.24049453323159356No Hit
TGAGGTAGTAGTTTGTACAGTT198160.23559618699412985No Hit
TCGTACGACTCTTAGCGG195620.23257633276035367No Hit
CCCCCCACTGCTAAATTTGACTGGCT194010.23066217318697585No Hit
TTAATGCTAATCGTGATAGGGGTTT192160.22846267305607582No Hit
CTTCTCACTACTGCACTTGACTAG190960.22703597026846506No Hit
CATTGCACTTGTCTCGGTCTGA188100.22363566195799264No Hit
CTCCCACTGCTTCACTTGACTAG186290.22148371858667967No Hit
TAGCTTATCAGACTGATGTTGACA183860.21859464544176782No Hit
CCCCCCACTGCTAAATTTGACTGG168530.20036851733003985No Hit
CACTAGATTGTGAGCTCCTGGA165430.1966828684620453No Hit
TCGTTTCCCGGCCAATGCACCA164690.19580306840968534No Hit
TGATGTAGTAGATTGTATAGTT163310.1941623602039329No Hit
TATTGCACTTGTCCCGGCCTGTTT149960.17829029169176275No Hit
TTCAAGTAATCCAGGATAGGCTT147210.1750207644701547No Hit
TGTAAACATCCCCGACTGGAAGC142190.16905239114198284No Hit
AACGGAATCCCAAAAGCAGCTG141230.16791102891189422No Hit
CCCATAAAGTAGAAAGCACT140310.16681722344139258No Hit
TGATAACTGAATTCCATAGGCTGT139230.16553319093254287No Hit
CTCCCACTGCTTCACTTGACTAGCCT136240.16197832315341262No Hit
TATTGCACTTGTCCCGGCCTGTT132680.15774577155016728No Hit
ATGCACCTGGGCAAGGATTCTGA131640.15650929580090456No Hit
ATCACATTGCCAGGGATTTCC128260.15249074961580084No Hit
AACATTCAACGCTGTCGGTGAGTT119360.1419093706076874No Hit
ACTTGACTTGGAGTCAGAAGGC117210.13935319477988473No Hit
TCGTTTCCCGGGCGGCGCACCA112810.13412195122531181No Hit
TTCAAGTAATCCAGGATAGGC112750.13405061608593127No Hit
ATCACATTGCCAGGGATTTCCA108020.12842702926476537No Hit
TGAGGTAGTAGTTTGTGCTGTT105930.1259421885763432No Hit
TTCACAGTGGCTAAGTTCTGC99020.11772675835768438No Hit
TTAATGCTAATCGTGATAGGGGT98540.11715607724264004No Hit
CCCTGTCTGAGCGTCGCT97300.11568181769544222No Hit
AGCAGCATTGTACAGGGCTATGT91240.1084769686180077No Hit
TTCACAGTGGCTAAGTTCCGC90370.10744260909698988No Hit
GACCCGCGGGCGCTCTCCAGTCCT89410.10630124686690123No Hit
ACTGGACTTGGAGTCAGAAGGCT89170.10601590630937908No Hit
CAAAGAATTCTCCTTTTGGGC88190.10485076569949692No Hit
TAGCAGCACATCATGGTTTACA88160.10481509812980666No Hit
TGTCAGTTTGTCAAATACCCCA87970.10458920352176829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)