FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7549325
Sequences flagged as poor quality0
Sequence length18-26
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCACGTTCCCGTGG182702924.20122328817477No Hit
CAACGGAATCCCAAAAGCAGCTG1954632.5891453871703765No Hit
GGAATACCGGGTGCTGTAGGCTT1772902.3484218787772417No Hit
GGAATACCGGGTGCTGTAGGCTTT1767892.3417855238713394No Hit
AAGCTGCCAGTTGAAGAACTGT1256761.6647316150781692No Hit
TCGTACGACTCTTAGCGG1087071.4399565524070033No Hit
TCGTTTCCCGGCCAATGCACCA1026791.3601083540581442No Hit
CCCCCCACTGCTAAATTTGACTG985441.3053352452040414No Hit
GCCTGTCTGAGCGTCGCT934891.2383756163630524No Hit
CTACGCCTGTCTGAGCGTCGCT818261.083884983094515No Hit
TAGCTTATCAGACTGATGTTGAC768351.0177731121656572No Hit
AACATTCAACGCTGTCGGTGAGT564760.7480933725862908No Hit
GCCACCACGTTCCCGTGG548140.7260781593056334No Hit
ACTGGACTTGGAGTCAGAAGGC528580.700168558116123No Hit
TGAATACCGGGTGCTGTAGGCTT480530.6365204836193964No Hit
TATTGCACTTGTCCCGGCCTGT473480.627181900368576No Hit
TGAATACCGGGTGCTGTAGGCTTT472690.626135449195789No Hit
AATTCCACCACGTTCCCGTGG444510.5888076086272614No Hit
TTCCACCACGTTCCCGTGG438380.5806876773751296No Hit
ATTCCACCACGTTCCCGTGG431200.5711768932984075No Hit
TACGCCTGTCTGAGCGTCGCT424570.5623946511774232No Hit
TTCAAGTAATCCAGGATAGGCT396490.525199272782666No Hit
CGCCTGTCTGAGCGTCGCT384820.5097409371036483No Hit
TGTAAACATCCTTGACTGGAAGCT375990.4980445271597129No Hit
ACCGGGTGCTGTAGGCTT356810.47263828223053056No Hit
TAGCTTATCAGACTGATGTTGA353860.4687306481043007No Hit
CCCCCACTGCTAAATTTGACTG309020.40933460938560734No Hit
GGAATACCGGGTGCTGTAGGCT307780.4076920784308531No Hit
TAGCAGCACGTAAATATTGGCG287100.3802989008951131No Hit
TTAATGCTAATCGTGATAGGGGTT284190.3764442516383915No Hit
CCTCGACACAAGGGTTTGT269980.3576213767456031No Hit
TGTAAACATCCCCGACTGGAAGCT254160.3366658608551096No Hit
AACATTCAACGCTGTCGGTGAG250170.3313806201216665No Hit
TCCTGTCTGAGCGTCGCT240680.3188099598308458No Hit
GGGCTACGCCTGTCTGAGCGTCGCT231150.3061863146705169No Hit
ACCGGGTGCTGTAGGCTTT221840.2938540862924831No Hit
GGAATACCGGGTGCTGTAGGCTTA219580.29086044116526977No Hit
GCTACGCCTGTCTGAGCGTCGCT213680.28304517291281006No Hit
ACGCCTGTCTGAGCGTCGCT210230.2784752279177277No Hit
TCGTTTCCCGGCCAACGCACCA205730.27251443009805515No Hit
TGAGGTAGTAGTTTGTGCTGTT204650.27108383862133373No Hit
TAGCTTATCAGACTGATGTTGACT204540.27093813023018615No Hit
TACCTGGTTGATCCTGCC204370.2707129445347763No Hit
GCGTTCGCGCTTTCCCCTG191060.2530822292059224No Hit
GAATTCCACCACGTTCCCGTGG187520.2483930682544466No Hit
CCCATAAAGTAGAAAGCACTAC179900.2382994506131343No Hit
ATACCGGGTGCTGTAGGCTT170540.22590099114821524No Hit
TATTGCACTTGTCCCGGCCTGTA165420.21911892785116552No Hit
CCGGGTGCTGTAGGCTTT165050.21862881780821464No Hit
AGCAGCATTGTACAGGGCTATGA160400.21246932672788627No Hit
CATAAAGTAGAAAGCACTACT160230.21224414103247644No Hit
AATACCGGGTGCTGTAGGCTT142410.18863938166657285No Hit
CCCCCACTGCTAAATTTGACTGGCTT138510.18347335688952324No Hit
ATACCGGGTGCTGTAGGCTTT127420.1687833018183745No Hit
CATTGCACTTGTCTCGGTCTGA126250.1672334943852596No Hit
CAACGGAATCCCAAAAGCAGCT121360.16075609408788202No Hit
CTCGACACAAGGGTTTGT120550.15968315048034096No Hit
TCGTTTCCCGGGCGGCGCACCA116770.15467608031181596No Hit
CAACGGAATCCCAAAAGCAGCTGT115740.15331171992197978No Hit
TACCGGGTGCTGTAGGCTT113650.15054326049017627No Hit
TATTGCACTTGTCCCGGCCTGTAA113390.15019885883837297No Hit
CCCCCCACTGCTAAATTTGACTGG112800.14941733201312699No Hit
TATTGCACTTGTCCCGGCCTGTAT102740.13609163733181445No Hit
CCTGTCTGAGCGTCGCTT100650.13332317790001091No Hit
AACCGGGCGGAAACACCA98630.13064744198984676No Hit
ACTGGACTTGGAGTCAGAAGG98390.13032953277279757No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT95890.127017978428535No Hit
AACATTCAACGCTGTCGGTGAGTT95350.12630268269017428No Hit
TCGATTCCCCGACGGGGAGCCA93380.12369317786689539No Hit
GAATACCGGGTGCTGTAGGCTT93220.12348123838886257No Hit
TAGCTTATCAGACTGATGTTGACA93020.12321631404132156No Hit
AAAAGCTGGGTTGAGAGGGCGA91200.12080550247869842No Hit
CCCCCCACTGCTAAATTTGACTGGCT89400.11842118335082938No Hit
AGCTACATTGTCTGCTGGGTTT87200.11550701552787832No Hit
CTGCGTTCGCGCTTTCCCCTG86090.11403668539902576No Hit
GGCTACGCCTGTCTGAGCGTCGCT85740.11357306779082897No Hit
AATACCGGGTGCTGTAGGCTTT83800.11100330161968122No Hit
TGAATACCGGGTGCTGTAGGCT83730.11091057809804188No Hit
TACCGGGTGCTGTAGGCTTT83110.11008931262066476No Hit
CAACACCAGTCGATGGGCTGT79750.10563858358197586No Hit
TGAGGTAGTAGGTTGTATAGTT79570.10540015166918898No Hit
GTCAGGATGGCCGAGCGGTCTAAGG77780.10302907875869698No Hit
CACTAGATTGTGAGCTCCTGGA75820.10043282015279512No Hit
CAAAGTGCTGTTCGTGCAGGTAG75760.10035334284853283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)