FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7596235
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6394408.41785437127735No Hit
CCCCCCACTGCTAAATTTGACTG5462567.191141400970349No Hit
GCCTGTCTGAGCGTCGCT4797936.3161947991340455No Hit
TTCAAGTAATCCAGGATAGGCT3324374.376339067972489No Hit
TAGCAGCACGTAAATATTGGCG2514933.3107585534149484No Hit
AAGCTGCCAGTTGAAGAACTGT2015582.6533934245056923No Hit
AACATTCAACGCTGTCGGTGAGT1959782.579935981443439No Hit
CCCCCACTGCTAAATTTGACTG1652412.1753013170340307No Hit
AACATTCAACGCTGTCGGTGAG1493121.9656053294823026No Hit
TGAGGTAGTAGGTTGTATAGTT1406931.8521412252253913No Hit
TAGCTTATCAGACTGATGTTGACT1129161.4864732331214083No Hit
TAGCTTATCAGACTGATGTTGAC1118571.4725321162391634No Hit
TATTGCACTTGTCCCGGCCTGTAA1086081.4297609276174315No Hit
TGAGGTAGTAGATTGTATAGTT965211.2706426275648397No Hit
TAGCTTATCAGACTGATGTTGA963361.2682072105457507No Hit
TATTGCACTTGTCCCGGCCTGTAT934801.2306096375375433No Hit
CATAAAGTAGAAAGCACTACT750900.9885160214237712No Hit
TGTAAACATCCTTGACTGGAAGCT744060.9795115606613013No Hit
TATTGCACTTGTCCCGGCCTGT729180.9599229091780336No Hit
TTCAAGTAATTCAGGATAGGTT723490.9524323562922947No Hit
CAACGGAATCCCAAAAGCAGCT488920.6436346426881212No Hit
TATTGCACTTGTCCCGGCCTGTA476050.626692038885053No Hit
AGCAGCATTGTACAGGGCTATGA446010.5871461322615743No Hit
CAACGGAATCCCAAAAGCAGCTGT392000.5160451197204932No Hit
CCCATAAAGTAGAAAGCACTAC388570.5115297249229388No Hit
TGAGGTAGTAGTTTGTACAGTT373390.4915461409500891No Hit
AACCGGGCGGAAACACCA373250.49136183912161746No Hit
ACTGGACTTGGAGTCAGAAGGC371290.4887816135230151No Hit
TGTAAACATCCCCGACTGGAAGCT352730.464348456834208No Hit
TCCTGTCTGAGCGTCGCT317560.41804920463887707No Hit
TCGTACGACTCTTAGCGG312190.410979913075359No Hit
CATTGCACTTGTCTCGGTCTGA291040.3831371725598273No Hit
CCCCCACTGCTAAATTTGACTGGCTT290050.3818338953442067No Hit
TCGTTTCCCGGCCAATGCACCA288140.37931949182720126No Hit
TTCTCACTACTGCACTTGACTAG285680.37608104541262877No Hit
CACCCGGCTGTGTGCACATGTGC267450.35208231446236193No Hit
CTCCCACTGCTTCACTTGACTAG243470.32051404412844No Hit
CCCATAAAGTAGAAAGCACT243200.3201586048878161No Hit
CGCCTGTCTGAGCGTCGCT236120.3108381981336807No Hit
CAACACCAGTCGATGGGCTGT201810.2656710857418182No Hit
TGTAAACATCCTACACTCTCAGCT200910.26448628827307213No Hit
TGAGGTAGTAGTTTGTGCTGTT194800.25644282990191847No Hit
CAAAGAATTCTCCTTTTGGGCT191240.251756297692212No Hit
CTTCTCACTACTGCACTTGACTAG184360.24269917926446455No Hit
TTCACAGTGGCTAAGTTCCG172710.2273626342523632No Hit
TAGCAGCACGTAAATATTGGC166760.21952980654232No Hit
AACGGAATCCCAAAAGCAGCTG161540.212657981223593No Hit
CCCCCCACTGCTAAATTTGACTGGCT155680.2049436332604244No Hit
TAGCTTATCAGACTGATGTTGACA153780.20244239415973833No Hit
TTCAAGTAATCCAGGATAGGCTT153610.20221859908230855No Hit
TGATGTAGTAGGTTGTATAGTT153540.20212644816807274No Hit
AGCTCGGTCTGAGGCCCCTCAGT152510.20077051328717452No Hit
TTCAAGTAATCCAGGATAGGC152000.20009912805488508No Hit
TTCACAGTGGCTAAGTTCTGC146210.1924769310059523No Hit
CAACGGAATCCCAAAAGCAGCTGA143510.1889225385997142No Hit
TTATAAAGCAATGAGACTGATT141230.18592105167889092No Hit
CACTAGATTGTGAGCTCCTGGA133740.1760609038556601No Hit
TATTGCACTTGTCCCGGCCTGTT131650.1733095408449054No Hit
TTCACAGTGGCTAAGTTCCGC124990.1645420395761848No Hit
AACATTCAACGCTGTCGGTGAGTT124420.16379166784597896No Hit
CAAAGAATTCTCCTTTTGGGCTT123230.16222510230397033No Hit
TGAGGTAGTAGGTTGTATAGT123050.16198814281022111No Hit
CTCCCACTGCTTCACTTGACTAGCCT120350.15843375040398303No Hit
CACTAGATTGTGAGCTCCTGGAA119490.15730161060051462No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC117090.15414215068385853No Hit
CCCCCCACTGCTAAATTTGACTGG115020.15141711650574263No Hit
TTCACAGTGGCTAAGTTCTG114460.15067990919185623No Hit
TATTGCACTTGTCCCGGCCTGTAG113430.14932397431095798No Hit
TGATGTAGTAGATTGTATAGTT106230.13984559456098974No Hit
AGCAGCATTGTACAGGGCTATGT105730.13918737374501974No Hit
TGAGGTAGTAGGTTGTGTGGTT104290.1372916977950261No Hit
AATGCACCCGGGCAAGGATTCT103840.13669929906065306No Hit
ATCCCGGACGAGCCCCCA103350.13605424266100247No Hit
CAAAGTGCTGTTCGTGCAGGT100160.13185479385511373No Hit
TGTAAACATCCCCGACTGGAAGC99600.13111758654122732No Hit
GGAATACCGGGTGCTGTAGGCTTT97570.12844521002838907No Hit
CTACGCCTGTCTGAGCGTCGCT96790.12741838555547585No Hit
ACCGGGTGCTGTAGGCTT93150.12262653801521411No Hit
ACCCCCCACTGCTAAATTTGACTG91230.12009897008188924No Hit
TTAATGCTAATCGTGATAGGGGTT90620.11929594068640582No Hit
CCCATAAAGTAGAAAGCACTA89990.11846658245828362No Hit
TGAGGTAGTAGATTGTATAGT89060.11724229174057937No Hit
TGAGGTAGTAAGTTGTATTGTT87770.11554408203537674No Hit
GTCAGGATGGCCGAGCGGTCTAAGG87060.11460940847669931No Hit
TGTCAGTTTGTCAAATACCCCA86640.1140565029912845No Hit
ATCACATTGCCAGGGATTTCC85020.11192386754754165No Hit
AGAGGTAGTAGGTTGCATAGTT84290.11096286515622542No Hit
TATTGCACTTGTCCCGGCCTGTAAA83930.110488946168727No Hit
TGAGAACTGAATTCCATAGGCTGT82210.10822466656179015No Hit
TATTGCACTTGTCCCGGCCTGTAAT81950.10788239173748573No Hit
ACTGGACTTGGAGTCAGAAGGCT80660.1061841820322831No Hit
AGCAGCATTGTACAGGGCTATG78900.10386724476006863No Hit
TATTGCACTTGTCCCGGCCTGTTT78540.10339332577257022No Hit
TGTCAGTTTGTCAAATACCCCAA77890.10253763871180921No Hit
TCGAGGAGCTCACAGTCTAGT77620.1021821994711854No Hit
AATGACACGATCACTCCCGTTG76820.10112904616563338No Hit
GACCCGCGGGCGCTCTCCAGTCCT76720.10099740200243937No Hit
CAAAGTGCTGTTCGTGCAGGTAG76620.10086575783924535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)