FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9469049
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT117284112.386048482799064No Hit
CAACGGAATCCCAAAAGCAGCTG7916508.360396065116992No Hit
CCCCCCACTGCTAAATTTGACTG6509016.873984916542305No Hit
TCCTGTCTGAGCGTCGCT3310793.49643348555911No Hit
TTCAAGTAATCCAGGATAGGCT2889933.0519749132146217No Hit
CCCCCACTGCTAAATTTGACTG2327302.457796976232777No Hit
TAGCAGCACGTAAATATTGGCG2310602.440160569451061No Hit
AAGCTGCCAGTTGAAGAACTGT1952652.0621395031327854No Hit
AACATTCAACGCTGTCGGTGAGT1514381.5992947127002932No Hit
TAGCTTATCAGACTGATGTTGACT1253691.3239872346209212No Hit
TAGCTTATCAGACTGATGTTGAC1165801.2311690434805016No Hit
AACATTCAACGCTGTCGGTGAG1148191.2125716109400215No Hit
TAGCTTATCAGACTGATGTTGA1031241.0890639598548915No Hit
TGAGGTAGTAGGTTGTATAGTT903380.9540345603872152No Hit
TATTGCACTTGTCCCGGCCTGTAA806560.8517856439437582No Hit
AACCGGGCGGAAACACCA796740.8414150143272044No Hit
TATTGCACTTGTCCCGGCCTGTAT706540.746157296260691No Hit
CATAAAGTAGAAAGCACTACT577470.6098500493555372No Hit
CCCTGTCTGAGCGTCGCT558070.5893622474654001No Hit
TCGTACGACTCTTAGCGG508610.5371289133681746No Hit
CAACGGAATCCCAAAAGCAGCTGT508270.5367698487989659No Hit
TGTAAACATCCTTGACTGGAAGCT492810.5204429716225991No Hit
TATTGCACTTGTCCCGGCCTGT491780.5193552171923496No Hit
TTCAAGTAATTCAGGATAGGTT479040.5059008565696513No Hit
TGAGGTAGTAGATTGTATAGTT472970.49949049793701567No Hit
CCCATAAAGTAGAAAGCACTAC425030.44886239367860487No Hit
CACCCGGCTGTGTGCACATGTGC357610.3776620017490669No Hit
AGCAGCATTGTACAGGGCTATGA352250.37200145442271976No Hit
TTCTCACTACTGCACTTGACTAG319480.33739396638458624No Hit
CCCCCACTGCTAAATTTGACTGGCTT310750.32817445553402463No Hit
TATTGCACTTGTCCCGGCCTGTA304760.3218485826823792No Hit
CTCCCACTGCTTCACTTGACTAG296950.31360065831320544No Hit
CATTGCACTTGTCTCGGTCTGA285910.3019416205365502No Hit
TGATGTAGTAGGTTGTATAGTT279080.2947286469845071No Hit
CGCCTGTCTGAGCGTCGCT270600.28577315419954No Hit
TGTAAACATCCCCGACTGGAAGCT262490.2772084081516528No Hit
ACTGGACTTGGAGTCAGAAGGC258630.273131969218873No Hit
CTTCTCACTACTGCACTTGACTAG224950.23756345542197532No Hit
CCCATAAAGTAGAAAGCACT212380.2242886270838814No Hit
ACCCCCCACTGCTAAATTTGACTG209790.22155339992432185No Hit
CAACACCAGTCGATGGGCTGT204620.2160935063278266No Hit
TAGCTTATCAGACTGATGTTGACA196200.20720137787860216No Hit
CAACGGAATCCCAAAAGCAGCT195930.20691623836776005No Hit
TGTAAACATCCTACACTCTCAGCT186860.19733766294798982No Hit
CCCCCCACTGCTAAATTTGACTGG183710.1940110353214985No Hit
TAGCAGCACGTAAATATTGGC182310.19253253415416902No Hit
ACCTGTCTGAGCGTCGCT177030.18695647260881215No Hit
CCCCCCACTGCTAAATTTGACTGGCT176520.18641787575499924No Hit
CAAAGAATTCTCCTTTTGGGCT167580.1769765897293382No Hit
CTACGCCTGTCTGAGCGTCGCT152800.16136784169138843No Hit
AGCTCGGTCTGAGGCCCCTCAGT152160.16069195544346637No Hit
TGAGGTAGTAGTTTGTACAGTT151150.15962532245846442No Hit
TGATGTAGTAGATTGTATAGTT145150.15328888888419523No Hit
ACCGGGTGCTGTAGGCTT144890.1530143100959769No Hit
CTCCCACTGCTTCACTTGACTAGCCT143540.15158861254176634No Hit
GCCTGTCTGAGCGTCGCTT141480.14941310368126726No Hit
AACGGAATCCCAAAAGCAGCTG139350.1471636697624017No Hit
TTCAAGTAATCCAGGATAGGCTT138590.14636105484299428No Hit
GCCGGTCTGAGCGTCGCT136120.14375255635492012No Hit
ACCGGGTGCTGTAGGCTTT133420.14090116124649898No Hit
TTCACAGTGGCTAAGTTCCGC128170.13535678186901345No Hit
GGAATACCGGGTGCTGTAGGCTTT126170.13324463734425707No Hit
ACCGGGTGCTGTAGGCTTA121860.12869296589340704No Hit
CGCGACCTCAGATCAGACGTGG113590.11995924828353936No Hit
TGAGGTAGTAGTTTGTGCTGTT113330.11968466949532101No Hit
CACTAGATTGTGAGCTCCTGGA112390.1186919615686855No Hit
CAAAGAATTCTCCTTTTGGGCTT109680.1158300057376406No Hit
TTCAAGTAATCCAGGATAGGC109460.1155976698399174No Hit
GCCTGTCTGAGCGTCGCG109350.1154815018910558No Hit
AACATTCAACGCTGTCGGTGAGTT109030.11514355876709478No Hit
TCCGGTCTGAGCGTCGCT107040.11304197496496216No Hit
ATCACATTGCCAGGGATTTCC105960.11190141692159371No Hit
TTCACAGTGGCTAAGTTCTGC104670.11053908370312585No Hit
CACTAGATTGTGAGCTCCTGGAA103980.10981039384208488No Hit
CCCCCCACTGCTAAATTTGACTGTA100520.10615638381425631No Hit
CCCCCCACTGCTAAATTTGACTGT96000.10138293718830688No Hit
TCGTTTCCCGGCCAATGCACCA95660.10102387261909829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)