FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9325999
Sequences flagged as poor quality0
Sequence length18-26
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT115357712.369473768976386No Hit
CAACGGAATCCCAAAAGCAGCTG6596527.073258317956071No Hit
CCCCCCACTGCTAAATTTGACTG5446765.840403800171971No Hit
TTCAAGTAATCCAGGATAGGCT2386282.5587392835877423No Hit
CCCCCACTGCTAAATTTGACTG2141322.2960757340848956No Hit
TCCTGTCTGAGCGTCGCT1572891.6865646243367602No Hit
AACATTCAACGCTGTCGGTGAGT1464601.5704483777019491No Hit
TAGCAGCACGTAAATATTGGCG1415621.517928535055601No Hit
AAGCTGCCAGTTGAAGAACTGT1318431.4137144985754342No Hit
GGAATACCGGGTGCTGTAGGCTTT1059211.1357603619730177No Hit
AACATTCAACGCTGTCGGTGAG1024611.0986597789684516No Hit
TCGTACGACTCTTAGCGG971661.0418830197172442No Hit
TGAGGTAGTAGGTTGTATAGTT902200.9674030631999854No Hit
AACCGGGCGGAAACACCA818070.8771928883972645No Hit
TAGCTTATCAGACTGATGTTGA731850.7847416668176782No Hit
GGAATACCGGGTGCTGTAGGCTT687040.7366931950132098No Hit
TAGCTTATCAGACTGATGTTGAC661050.7088248669123812No Hit
TAGCTTATCAGACTGATGTTGACT616500.6610551856160396No Hit
CGCCTGTCTGAGCGTCGCT523190.5610015613340726No Hit
TATTGCACTTGTCCCGGCCTGTAA513820.5509543803296569No Hit
CATAAAGTAGAAAGCACTACT428850.45984349773144945No Hit
TATTGCACTTGTCCCGGCCTGTAT427520.45841737705526237No Hit
ACCGGGTGCTGTAGGCTT426340.4571520970568408No Hit
TATTGCACTTGTCCCGGCCTGT419270.44957113977816215No Hit
TGAGGTAGTAGATTGTATAGTT416040.4461077038502792No Hit
CAACGGAATCCCAAAAGCAGCTGT400120.42903714658343844No Hit
ACCGGGTGCTGTAGGCTTT369290.39597902594671086No Hit
ACTGGACTTGGAGTCAGAAGGC337910.36233115615817674No Hit
CACCCGGCTGTGTGCACATGTGC337640.3620416429381989No Hit
TTCAAGTAATTCAGGATAGGTT326370.34995714668208733No Hit
ACCGGGTGCTGTAGGCTTA311420.33392669246479656No Hit
AGCAGCATTGTACAGGGCTATGA279790.3000107548799866No Hit
TCCTTCTGATCGAGGCCC277910.29799488505199284No Hit
CCCATAAAGTAGAAAGCACTAC277310.297351522340931No Hit
CATTGCACTTGTCTCGGTCTGA267340.2866609786254534No Hit
CTACGCCTGTCTGAGCGTCGCT254450.2728394030494749No Hit
CTCCCACTGCTTCACTTGACTAG249380.2674029881410024No Hit
CCCCCACTGCTAAATTTGACTGGCTT240170.2575273705262031No Hit
CGCGACCTCAGATCAGACGTGG235190.25218746002438985No Hit
TATTGCACTTGTCCCGGCCTGTA229720.2463221366418761No Hit
TGTAAACATCCTTGACTGGAAGCT228190.24468156172866842No Hit
CAACACCAGTCGATGGGCTGT217220.2329187468280878No Hit
TTCTCACTACTGCACTTGACTAG216150.2317714166600275No Hit
TGAGAACTGAATTCCATGGGTT209760.22491960378721895No Hit
CCCATAAAGTAGAAAGCACT207090.22205663972299375No Hit
CCCTGTCTGAGCGTCGCT188430.20204805940897055No Hit
CGCGACCTCAGATCAGACGTGGC186790.2002895346654015No Hit
TGAATACCGGGTGCTGTAGGCTTT184850.19820932856630158No Hit
CAACGGAATCCCAAAAGCAGCT174900.1875402302745261No Hit
ATCCCGGACGAGCCCCCA170350.18266139638230713No Hit
AACGGAATCCCAAAAGCAGCTG163040.17482309401920373No Hit
TACGCCTGTCTGAGCGTCGCT159580.17111303571874714No Hit
ACCCCCCACTGCTAAATTTGACTG156700.1680248947056503No Hit
CCCCCCACTGCTAAATTTGACTGG156690.16801417199379928No Hit
GGAATACCGGGTGCTGTAGGCTTA153220.16429339098149165No Hit
TGTAAACATCCCCGACTGGAAGCT147580.15824578149751034No Hit
TTAATGCTAATCGTGATAGGGGTT145400.15590823031398565No Hit
AGCTCGGTCTGAGGCCCCTCAGT144310.1547394547222233No Hit
CGCGACCTCAGATCAGACGTGGCG143870.15426765540077797No Hit
ACGCCTGTCTGAGCGTCGCT137720.14767318761239412No Hit
CCCCCCACTGCTAAATTTGACTGGCT134560.14428481066746843No Hit
CTTCTCACTACTGCACTTGACTAG130170.13957754016486598No Hit
TGAGGTAGTAGTTTGTACAGTT125870.1349667740689228No Hit
TCCGGTCTGAGCGTCGCT123020.13191080119137907No Hit
TGAATACCGGGTGCTGTAGGCTT121440.1302166127189162No Hit
GACCCGCGGGCGCTCTCCAGTCCT118920.12751448933245652No Hit
TGAGGTAGTAGGTTGTGTGGTT117220.12569162831778127No Hit
TACCACCCTGAACGCGCCCGA116880.12532705611484624No Hit
TAGCAGCACGTAAATATTGGC115670.12402960798087154No Hit
TGTAAACATCCTACACTCTCAGCT110040.11799272120874128No Hit
GGAATACCGGGTGCTGTAGGCT107290.11504397544970786No Hit
CGCGACCTCAGATCAGACGT106880.11460434426381559No Hit
TGAGGTAGTAGTTTGTGCTGTT106400.11408965409496613No Hit
CACTAGATTGTGAGCTCCTGGA105620.11325328257058573No Hit
AACATTCAACGCTGTCGGTGAGTT104130.11165559850478217No Hit
TTCAAGTAATCCAGGATAGGCTT103960.11147331240331465No Hit
TGATGTAGTAGGTTGTATAGTT99960.10718422766290239No Hit
ACCACCCTGAACGCGCCCGA99790.10700194156143486No Hit
TTCACAGTGGCTAAGTTCCGC98460.10557582088524778No Hit
TCGTTTCCCGGCCAATGCACCA97860.10493245817418595No Hit
TTCAAGTAATCCAGGATAGGC97780.1048466764793777No Hit
GCCGGTCTGAGCGTCGCT97050.10406391851425247No Hit
CCCCCCACTGCTAAATTTGACTGTA95870.10279863851583085No Hit
ACTGGACTTGGAGTCAGAAGGCT94880.10173709004257883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)