FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9330724
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8614599.232499000077594No Hit
CAACGGAATCCCAAAAGCAGCTG8092328.672767515146734No Hit
TTCAAGTAATCCAGGATAGGCT3311803.5493494395504572No Hit
AAGCTGCCAGTTGAAGAACTGT2991023.2055604688339296No Hit
TAGCAGCACGTAAATATTGGCG2780942.9804118094158607No Hit
CCCCCACTGCTAAATTTGACTG2721972.9172119976970707No Hit
GCCTGTCTGAGCGTCGCT2063102.2110824411910586No Hit
AACATTCAACGCTGTCGGTGAGT1660981.7801190990109663No Hit
TAGCTTATCAGACTGATGTTGACT1503631.6114826673685774No Hit
TGAGGTAGTAGGTTGTATAGTT1502671.6104538083004063No Hit
TAGCTTATCAGACTGATGTTGAC1450471.5545095964686129No Hit
AACATTCAACGCTGTCGGTGAG1413611.5150056951636337No Hit
TATTGCACTTGTCCCGGCCTGTAT1362651.4603904262948941No Hit
TATTGCACTTGTCCCGGCCTGTAA1342671.438977296938587No Hit
TAGCTTATCAGACTGATGTTGA1095541.174121107858297No Hit
TGAGGTAGTAGATTGTATAGTT914980.9806098647864839No Hit
TGTAAACATCCTTGACTGGAAGCT883540.9469147303038864No Hit
TATTGCACTTGTCCCGGCCTGT807300.8652061726399796No Hit
TCCTGTCTGAGCGTCGCT686910.7361808151221706No Hit
TATTGCACTTGTCCCGGCCTGTA595670.6383963345180931No Hit
TTCAAGTAATTCAGGATAGGTT561080.6013252562180599No Hit
CATAAAGTAGAAAGCACTACT556720.5966525212834503No Hit
CAACGGAATCCCAAAAGCAGCTGT545530.5846598827700831No Hit
CCCATAAAGTAGAAAGCACTAC500760.5366786114346539No Hit
TGTAAACATCCCCGACTGGAAGCT438830.4703064842556698No Hit
AGCAGCATTGTACAGGGCTATGA431340.46227924006754456No Hit
TGAGAACTGAATTCCATGGGTT417490.4474358045527871No Hit
TTCTCACTACTGCACTTGACTAG377420.40449165573861146No Hit
TTAATGCTAATCGTGATAGGGGTT373870.40068702064277106No Hit
ACTGGACTTGGAGTCAGAAGGC371680.39833993589350625No Hit
CACCCGGCTGTGTGCACATGTGC351000.3761765967999911No Hit
TGAGGTAGTAGTTTGTACAGTT325600.3489547006213023No Hit
CCCCCACTGCTAAATTTGACTGGCTT308610.3307460385710691No Hit
GGAATACCGGGTGCTGTAGGCTTT296420.31768167186169044No Hit
CTCCCACTGCTTCACTTGACTAG285370.3058390752957648No Hit
CATTGCACTTGTCTCGGTCTGA284540.3049495408930754No Hit
ACCCCCCACTGCTAAATTTGACTG278360.2983262606417251No Hit
TAGCTTATCAGACTGATGTTGACA259710.27833852978611306No Hit
TGTAAACATCCTACACTCTCAGCT247380.26512412112929284No Hit
TCGTACGACTCTTAGCGG240730.25799712862581725No Hit
CTTCTCACTACTGCACTTGACTAG236710.25368878127785155No Hit
TGAGGTAGTAGTTTGTGCTGTT218550.23422619723828506No Hit
TAGCAGCACGTAAATATTGGC212150.2273671367838123No Hit
TGAGAACTGAATTCCATAGGCTGT207910.22282300923272408No Hit
ACCGGGTGCTGTAGGCTTA206330.22112967868302608No Hit
CAAAGAATTCTCCTTTTGGGCT205280.22000436407721416No Hit
GGAATACCGGGTGCTGTAGGCTT205110.21982217028389223No Hit
TGATGTAGTAGGTTGTATAGTT202440.21696065600054185No Hit
AACGGAATCCCAAAAGCAGCTG199160.2134453875176246No Hit
ACCGGGTGCTGTAGGCTT191180.20489299651345383No Hit
AACCGGGCGGAAACACCA190950.20464649902837123No Hit
ACCGGGTGCTGTAGGCTTT190000.20362835724216044No Hit
CCCCCCACTGCTAAATTTGACTGG181900.19494735885446832No Hit
CCCATAAAGTAGAAAGCACT181770.19480803418898684No Hit
CAACGGAATCCCAAAAGCAGCT178400.19119631016842853No Hit
AGCTCGGTCTGAGGCCCCTCAGT163990.17575270686390468No Hit
TATTGCACTTGTCCCGGCCTGTAG162500.17415583185184771No Hit
TATTGCACTTGTCCCGGCCTGTT161300.17286975801663407No Hit
TTCAAGTAATCCAGGATAGGCTT157860.16918301302235497No Hit
TTCAAGTAATCCAGGATAGGC148580.15923737536336946No Hit
CAACACCAGTCGATGGGCTGT148030.15864792485556317No Hit
TCGTTTCCCGGCCAATGCACCA145970.15644016477177974No Hit
TTCACAGTGGCTAAGTTCCGC145160.15557206493301057No Hit
CTCCCACTGCTTCACTTGACTAGCCT144500.15486472432364307No Hit
CCCCCCACTGCTAAATTTGACTGGCT142720.1529570481347428No Hit
CACTAGATTGTGAGCTCCTGGA137620.14749123433508482No Hit
CAAAGAATTCTCCTTTTGGGCTT135270.1449726730744581No Hit
TGTAAACATCCCCGACTGGAAGC135040.1447261755893755No Hit
TTCACAGTGGCTAAGTTCTGC132360.141853944024065No Hit
CACTAGATTGTGAGCTCCTGGAA131980.14144668730958068No Hit
TGTCAGTTTGTCAAATACCCCA127890.13706331898789417No Hit
CGCGACCTCAGATCAGACGTGG127730.13689184247653235No Hit
TGATGTAGTAGATTGTATAGTT126930.13603445991972327No Hit
CCCCCCACTGCTAAATTTGACTGT126100.13514492551703383No Hit
GACCCGCGGGCGCTCTCCAGTCCT124600.13353733322301678No Hit
TGAGGTAGTAAGTTGTATTGTT122450.13123311760159234No Hit
CGCGACCTCAGATCAGACGTGGCG121840.1305793634020254No Hit
TGAGGTAGTAGGTTGTATAGT117170.1255743927266523No Hit
TGAGGTAGTAGGTTGTGTGGTT116040.12436333986515945No Hit
ATCACATTGCCAGGGATTTCC115860.1241704287898774No Hit
TATTGCACTTGTCCCGGCCTGTTT114470.12268072659742159No Hit
TATTGCACTTGTCCCGGCCTGTAAT113630.12178047491277204No Hit
AACATTCAACGCTGTCGGTGAGTT113050.12115887255908546No Hit
TGTCAGTTTGTCAAATACCCCAA112840.12093380963792306No Hit
CCCCCCACTGCTAAATTTGACTGTA112710.1207944849724416No Hit
TGAATACCGGGTGCTGTAGGCTTT112700.12078376769048148No Hit
ACTGGACTTGGAGTCAGAAGGCT112030.12006570979915385No Hit
ATGCACCTGGGCAAGGATTCTGA106480.11411761831129075No Hit
CGCCTGTCTGAGCGTCGCT105810.11339956041996312No Hit
CTACGCCTGTCTGAGCGTCGCT103380.11079526090365549No Hit
TATTGCACTTGTCCCGGCCTGTAAA100930.10816952682342763No Hit
TAGCAGCACGTAAATATTGGCGT97940.10496505951735363No Hit
ACTGGACTTGGAGTCAGAAGGCA97930.10495434223539353No Hit
TCGTTTCCCGGGCGGCGCACCA94810.10161055026383806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)