FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7389711
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5479227.414660735717541No Hit
CAACGGAATCCCAAAAGCAGCTG2916933.947285624566373No Hit
AAGCTGCCAGTTGAAGAACTGT2178312.947760744635345No Hit
GCCTGTCTGAGCGTCGCT2072262.8042503962604224No Hit
TTCAAGTAATCCAGGATAGGCT1909402.583862887195453No Hit
CCCCCACTGCTAAATTTGACTG1620962.193536391341962No Hit
TAGCAGCACGTAAATATTGGCG1591062.153074727820885No Hit
AACATTCAACGCTGTCGGTGAGT1544982.0907177560800414No Hit
TAGCTTATCAGACTGATGTTGAC1403601.8993976895713514No Hit
TAGCTTATCAGACTGATGTTGACT1123931.5209390461954466No Hit
TAGCTTATCAGACTGATGTTGA921211.2466116739883333No Hit
TATTGCACTTGTCCCGGCCTGTAT834471.1292322527904No Hit
TATTGCACTTGTCCCGGCCTGTAA822441.1129528610794117No Hit
AACATTCAACGCTGTCGGTGAG810251.0964569521054341No Hit
AACCGGGCGGAAACACCA744621.0076442772931173No Hit
TATTGCACTTGTCCCGGCCTGT714050.966275947733274No Hit
TGAGGTAGTAGGTTGTATAGTT710720.9617696821973146No Hit
TGTAAACATCCTTGACTGGAAGCT611620.8276643024334781No Hit
CGCCTGTCTGAGCGTCGCT512130.6930311618411058No Hit
TGAGGTAGTAGATTGTATAGTT465520.6299569766666112No Hit
TATTGCACTTGTCCCGGCCTGTA462540.6259243426434403No Hit
TCGTACGACTCTTAGCGG430110.5820389999013493No Hit
TCCTGTCTGAGCGTCGCT429510.5812270601651405No Hit
AGCAGCATTGTACAGGGCTATGA419900.5682224920568613No Hit
CATAAAGTAGAAAGCACTACT393740.5328219195581532No Hit
ACCGGGTGCTGTAGGCTT390380.5282750570353834No Hit
GGAATACCGGGTGCTGTAGGCTTT388370.5255550589190835No Hit
TTCAAGTAATTCAGGATAGGTT378630.5123745705346258No Hit
ACCGGGTGCTGTAGGCTTT340140.46028863645682494No Hit
ACCGGGTGCTGTAGGCTTA333210.45091073250361213No Hit
TTAATGCTAATCGTGATAGGGGTT331340.44838018699242776No Hit
ACTGGACTTGGAGTCAGAAGGC314370.4254158247866527No Hit
TGTAAACATCCCCGACTGGAAGCT302360.4091634977335379No Hit
GGAATACCGGGTGCTGTAGGCTT281660.3811515768343309No Hit
TTCTCACTACTGCACTTGACTAG257900.3489987632804585No Hit
CTACGCCTGTCTGAGCGTCGCT222240.3007424782917762No Hit
CCCATAAAGTAGAAAGCACTAC214510.29028198802361826No Hit
CAACGGAATCCCAAAAGCAGCTGT203540.27543702317993224No Hit
CATTGCACTTGTCTCGGTCTGA199850.2704435938022475No Hit
TGTAAACATCCTACACTCTCAGCT197320.2670199145812333No Hit
TACGCCTGTCTGAGCGTCGCT196770.2662756364897084No Hit
CTCCCACTGCTTCACTTGACTAG191630.2593200194161856No Hit
TAGCTTATCAGACTGATGTTGACA190890.25831862707486125No Hit
TGAGGTAGTAGTTTGTACAGTT180010.24359545319160655No Hit
CTTCTCACTACTGCACTTGACTAG175720.2377900840777129No Hit
CACCCGGCTGTGTGCACATGTGC166800.22571924666607396No Hit
ATCCCGGACGAGCCCCCA165890.2244878047328238No Hit
ACGCCTGTCTGAGCGTCGCT161960.2191695994606555No Hit
AACATTCAACGCTGTCGGTGAGTT150660.20387806776205455No Hit
TGAGGTAGTAGTTTGTGCTGTT137700.18634016945994236No Hit
CCCCCCACTGCTAAATTTGACTGG134380.18184743625291977No Hit
GGGCTACGCCTGTCTGAGCGTCGCT132870.1798040545834607No Hit
AGCTCGGTCTGAGGCCCCTCAGT132760.17965519896515575No Hit
CCCATAAAGTAGAAAGCACT131120.1774358970195181No Hit
TTCAAGTAATCCAGGATAGGCTT130130.1760961964547734No Hit
TTCACAGTGGCTAAGTTCCG129190.1748241575347128No Hit
TATTGCACTTGTCCCGGCCTGTT126220.17080505584047875No Hit
CGCGACCTCAGATCAGACGTGGCG121250.1640794883588817No Hit
CAACACCAGTCGATGGGCTGT121070.163835906438019No Hit
CCCCCACTGCTAAATTTGACTGGCTT119600.1618466540843072No Hit
ACCCCCCACTGCTAAATTTGACTG119490.16169779846600224No Hit
GCGTTCGCGCTTTCCCCTG119230.16134595791364506No Hit
CGCGACCTCAGATCAGACGTGG112960.15286118767026208No Hit
GACCCGCGGGCGCTCTCCAGTCCT106110.1435915423485438No Hit
TGTCAGTTTGTCAAATACCCCAA102970.1393423910623839No Hit
CAAAGAATTCTCCTTTTGGGCT100140.13551274197326524No Hit
TTATAAAGCAATGAGACTGATT99330.13441662332938326No Hit
TGATGTAGTAGGTTGTATAGTT98770.1336588129089216No Hit
GGGAATACCGGGTGCTGTAGGCTTT98590.13341523098805894No Hit
TATTGCACTTGTCCCGGCCTGTAG95760.12958558189894032No Hit
TGAATACCGGGTGCTGTAGGCTTT94030.1272444889928713No Hit
CCCCCCACTGCTAAATTTGACTGTA93560.126608469532841No Hit
TGTCAGTTTGTCAAATACCCCA92440.12509284869191772No Hit
CCCCCCACTGCTAAATTTGACTGGCT92290.12488986375786551No Hit
GGGAATACCGGGTGCTGTAGGCTT91690.1240779240216566No Hit
AGCAGCATTGTACAGGGCTATGT90070.12188568673389256No Hit
CAACGGAATCCCAAAAGCAGCT89490.1211008116555573No Hit
TAGCAGCACGTAAATATTGGC89330.12088429439256826No Hit
CACTAGATTGTGAGCTCCTGGA89300.12084369740575782No Hit
TCGTTTCCCGGCCAATGCACCA88290.11947693218313951No Hit
CCCCCCACTGCTAAATTTGACTGT88260.11943633519632904No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT87960.1190303653282246No Hit
TGTAAACATCCTACACTCAGCT87770.11877325107842512No Hit
TGTAAACATCCCCGACTGGAAGC86390.11690578968514467No Hit
TTCAAGTAATCCAGGATAGGC85300.11543076583103182No Hit
GAATACCGGGTGCTGTAGGCTTT84210.11395574197691899No Hit
TGAGGTAGTAGGTTGTGTGGTT83000.11231833017556438No Hit
TCGTTTCCCGGGCGGCGCACCA82250.11130340550530327No Hit
ACCACAGGGTAGAACCACGGAC81190.10986897863800085No Hit
CACTAGATTGTGAGCTCCTGGAA79270.10727077148213239No Hit
CAAAGAATTCTCCTTTTGGGCTT77640.10506500186543155No Hit
CCTCGACACAAGGGTTTGT77070.1042936591160331No Hit
CCAAGTCCTTCTGATCGAGGCCC77040.10425306212922265No Hit
TATTGCACTTGTCCCGGCCTGTTT74700.10108649715800794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)